Cited by 38Metabolism, Diabetes, and CancerDiabetes, Cardiovascular Risks, and LipoproteinsAdipose Tissue and Metabolism

The reduced insulin-mediated glucose oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin—evidence from cultured myotubes

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Gaster, Michael, Beck-Nielsen, Henning·2004·Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

Abstract

Several defects in response to insulin have been described in vivo and in vitro in type 2 diabetes: a decreased glucose transport, defective glucose oxidation and altered glycogen synthesis. At present, it is unknown whether glucose oxidation is primarily affected or secondarily affected by, e.g. increased free fatty acids (FFA). The aim of this study was to evaluate whether myotubes established from type 2 diabetic subjects express a primarily or a FFA-induced reduced insulin-mediated glucose oxidation. We have therefore investigated glucose oxidation under basal, physiological conditions and during acute insulin stimulation with/without FFA. We found that myotubes established from type 2 diabetic subjects express a reduced insulin-stimulated increase in glucose oxidation. Moreover, an acute exposure to FFA reduces insulin-mediated glucose oxidation without alterations in glucose uptake and glycogen synthesis. Thus, we conclude that the diminished increase in insulin-stimulated glucose oxidation seen in type 2 diabetic subjects in vivo may be of genetic origin. Moreover, the glucose-fatty acid cycle seems not to be crucial for the pathophysiology of insulin resistance.

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Authors
Gaster, M., & Beck-Nielsen, H.
Affiliations
Odense University Hospital (DK)
Corresponding author
Gaster, Michael
Venue
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Publisher
Elsevier BV
Published
2004-09
Volume
1690
Issue
1
Pages
85-91
ISSN
0925-4439 (ISSN-L)
OpenAlex
W2160636742
Cited by
38
References
30
Citations by year
2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 4
FWCI
0.95
Type
journal article
Language
en
Open access
Locations
  • Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (publishedVersion)
  • PubMed (publishedVersion)
  • University of Southern Denmark Research Portal (University of Southern Denmark) (submittedVersion)
Funders
  • Odense Universitetshospital
  • Novo Nordisk Fonden
  • Duke Clinical Research Institute
  • Sundhed og Sygdom, Det Frie Forskningsråd
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
type 2 diabetes, insulin resistance, skeletal muscle, glucose oxidation, myotubes, free fatty acids, glucose-fatty acid cycle, insulin-stimulated glucose uptake, glycogen synthesis
MeSH terms
Cells, Cultured, Diabetes Mellitus, Type 2, Fatty Acids, Nonesterified, Glucose, Glycogen, Humans, Insulin, Oxidation-Reduction, Muscle, Skeletal, Muscle Fibers, Skeletal, Palmitic Acid

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