The reduced insulin-mediated glucose oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin—evidence from cultured myotubes
Open accessAbstract
Several defects in response to insulin have been described in vivo and in vitro in type 2 diabetes: a decreased glucose transport, defective glucose oxidation and altered glycogen synthesis. At present, it is unknown whether glucose oxidation is primarily affected or secondarily affected by, e.g. increased free fatty acids (FFA). The aim of this study was to evaluate whether myotubes established from type 2 diabetic subjects express a primarily or a FFA-induced reduced insulin-mediated glucose oxidation. We have therefore investigated glucose oxidation under basal, physiological conditions and during acute insulin stimulation with/without FFA. We found that myotubes established from type 2 diabetic subjects express a reduced insulin-stimulated increase in glucose oxidation. Moreover, an acute exposure to FFA reduces insulin-mediated glucose oxidation without alterations in glucose uptake and glycogen synthesis. Thus, we conclude that the diminished increase in insulin-stimulated glucose oxidation seen in type 2 diabetic subjects in vivo may be of genetic origin. Moreover, the glucose-fatty acid cycle seems not to be crucial for the pathophysiology of insulin resistance.
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In culture, muscle cells taken from insulin-resistant people stay insulin resistant.
“Muscle cells from insulin-resistant individuals remain insulin resistant in vitro (39-42)”
From Dynamic changes in fat oxidation in human primary myocytes mirror metabolic characteristics of the donor (2005) · cites this paper as [42]
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- Authors
- Gaster, M., & Beck-Nielsen, H.
- Affiliations
- Odense University Hospital (DK)
- Corresponding author
- Gaster, Michael
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
- Publisher
- Elsevier BV
- Published
- 2004-09
- Volume
- 1690
- Issue
- 1
- Pages
- 85-91
- ISSN
- 0925-4439 (ISSN-L)
- OpenAlex
- W2160636742
- Cited by
- 38
- References
- 30
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 4
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (publishedVersion)
- PubMed (publishedVersion)
- University of Southern Denmark Research Portal (University of Southern Denmark) (submittedVersion)
- Funders
- Odense Universitetshospital
- Novo Nordisk Fonden
- Duke Clinical Research Institute
- Sundhed og Sygdom, Det Frie Forskningsråd
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- type 2 diabetes, insulin resistance, skeletal muscle, glucose oxidation, myotubes, free fatty acids, glucose-fatty acid cycle, insulin-stimulated glucose uptake, glycogen synthesis
- MeSH terms
- Cells, Cultured, Diabetes Mellitus, Type 2, Fatty Acids, Nonesterified, Glucose, Glycogen, Humans, Insulin, Oxidation-Reduction, Muscle, Skeletal, Muscle Fibers, Skeletal, Palmitic Acid
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