Transcriptional profiling of myotubes from patients with type 2 diabetes: no evidence for a primary defect in oxidative phosphorylation genes.
Abstract
Aims/hypothesis Microarray-based studies of skeletal muscle from patients with type 2 diabetes and high-risk individuals have demonstrated that insulin resistance and reduced mitochondrial biogenesis co-exist early in the pathogenesis of type 2 diabetes independently of hyperglycaemia and obesity. It is unknown whether reduced mitochondrial biogenesis or other transcriptional alterations co-exist with impaired insulin responsiveness in primary human muscle cells from patients with type 2 diabetes.Methods Using cDNA microarray technology and global pathway analysis with the Gene Map Annotator and Pathway Profiler (GenMapp 2.1) and Gene Set Enrichment Analysis (GSEA 2.0.1), we examined transcript levels in myotubes established from obese patients with type 2 diabetes and matched obese healthy participants, who had been extensively metabolically characterised both in vivo and in vitro. We have previously reported reduced basal lipid oxidation and impaired insulin-stimulated glycogen synthesis and glucose oxidation in these diabetic myotubes.Results No single gene was differently expressed after correction for multiple testing, and no biological pathway was differently expressed using either method of global pathway analysis. In particular, we found no evidence for differential expression of genes involved in mitochondrial oxidative metabolism. Consistently, there was no difference in mRNA levels of genes known to mediate the transcriptional control of mitochondrial biogenesis (PPARGC1A and NRF1) or in mitochondrial mass between diabetic and control myotubes.Conclusions/interpretation These results support the hypothesis that impaired mitochondrial biogenesis is not a primary defect in the sequence of events leading to insulin resistance and type 2 diabetes.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Primary myotubes isolated from obese humans with type 2 DM display reduced basal lipid oxidation and insulin-stimulated glucose oxidation with no differences in OXPHOS gene expression.
“For example, primary myotubes isolated from obese humans with type 2 DM display reduced basal lipid oxidation and insulinstimulated glucose oxidation with no differences in OXPHOS gene expression (126).”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [126]
PGC-1α expression may also be reduced as a consequence of promoter methylation or caused by insulin itself, obesity, and sustained lipid exposure.
“In turn, PGC-1␣ expression may also be reduced as a consequence of promoter methylation (146) or caused by insulin itself (145), obesity (126), and sustained lipid exposure (126).”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [126]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Bioconductor: open software development for computational biology and bioinformatics
Gentleman, R., Carey, V., Bates, D., Bolstad, B., Dettling, M., Dudoit, S., Ellis, B., Gautier, L., Ge, Y., Gentry, J., et al. (2004) · Genome Biology · doi:10.1186/gb-2004-5-10-r80 · Cited by 13,133 · see citing works
Exploration, normalization, and summaries of high density oligonucleotide array probe level data
Irizarry, R. A. (2003) · Biostatistics · doi:10.1093/biostatistics/4.2.249 · Cited by 10,603 · see citing works
Mootha, V. K., Lindgren, C. M., Eriksson, K.-F., Subramanian, A., Sihag, S., Lehar, J., Puigserver, P., Carlsson, E., Ridderstråle, M., Laurila, E., et al. (2003) · Nature Genetics · doi:10.1038/ng1180 · Cited by 10,197 · see citing works
Dysfunction of Mitochondria in Human Skeletal Muscle in Type 2 Diabetes
Kelley, D. E., He, J., Menshikova, E. V., & Ritov, V. B. (2002) · Diabetes · doi:10.2337/diabetes.51.10.2944 · Cited by 2,321 · see citing works
Impaired Mitochondrial Activity in the Insulin-Resistant Offspring of Patients with Type 2 Diabetes
Petersen, K. F., Dufour, S., Befroy, D., Garcia, R., & Shulman, G. I. (2004) · New England Journal of Medicine · doi:10.1056/nejmoa031314 · Cited by 2,193 · see citing works
Mitochondrial dysfunction in the elderly: possible role in insulin resistance
Petersen, K. F., Befroy, D., Dufour, S., Dziura, J., Ariyan, C., Rothman, D. L., DiPietro, L., Cline, G. W., & Shulman, G. I. (2003) · Science · doi:10.1126/science.1082889 · Cited by 2,107 · see citing works
Patti, M. E., Butte, A. J., Crunkhorn, S., Cusi, K., Berria, R., Kashyap, S., Miyazaki, Y., Kohane, I., Costello, M., Saccone, R., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1032913100 · Cited by 1,952 · see citing works
Cusi, K., Maezono, K., Osman, A., Pendergrass, M., Patti, M. E., Pratipanawatr, T., DeFronzo, R. A., Kahn, C. R., & Mandarino, L. J. (2000) · Journal of Clinical Investigation · doi:10.1172/jci7535 · Cited by 1,139 · see citing works
Deficiency of subsarcolemmal mitochondria in obesity and type 2 diabetes
Ritov, V. B., Menshikova, E. V., He, J., Ferrell, R. E., Goodpaster, B. H., & Kelley, D. E. (2005) · Diabetes · doi:10.2337/diabetes.54.1.8 · Cited by 887 · see citing works
Bonnard, C., Durand, A., Peyrol, S., Chanseaume, E., Chauvin, M.-A., Morio, B., Vidal, H., & Rieusset, J. (2008) · Journal of Clinical Investigation · doi:10.1172/jci32601 · Cited by 819 · see citing works
Mitochondrial respiration is decreased in skeletal muscle of patients with type 2 diabetes
Mogensen, M., Sahlin, K., Fernström, M., Glintborg, D., Vind, B. F., Beck-Nielsen, H., & Højlund, K. (2007) · Diabetes · doi:10.2337/db06-0981 · Cited by 573 · see citing works
Self-citationPatients with type 2 diabetes have normal mitochondrial function in skeletal muscle
Boushel, R., Gnaiger, E., Schjerling, P., Skovbro, M., Kraunsøe, R., & Dela, F. (2007) · Diabetologia · doi:10.1007/s00125-007-0594-3 · Cited by 519 · see citing works
Stump, C. S., Short, K. R., Bigelow, M. L., Schimke, J. M., & Nair, K. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1332551100 · Cited by 477 · see citing works
Befroy, D. E., Petersen, K. F., Dufour, S., Mason, G. F., de Graaf, R. A., Rothman, D. L., & Shulman, G. I. (2007) · Diabetes · doi:10.2337/db06-0783 · Cited by 453 · see citing works
Toledo, F. G. S., Menshikova, E. V., Ritov, V. B., Azuma, K., Radikova, Z., DeLany, J., & Kelley, D. E. (2007) · Diabetes · doi:10.2337/db07-0141 · Cited by 321 · see citing works
Petersen, K. F., Dufour, S., & Shulman, G. I. (2005) · PLoS Medicine · doi:10.1371/journal.pmed.0020233 · Cited by 308 · see citing works
Bouzakri, K., Roques, M., Gual, P., Espinosa, S., Guebre-Egziabher, F., Riou, J.-P., Laville, M., Le Marchand-Brustel, Y., Tanti, J.-F., & Vidal, H. (2003) · Diabetes · doi:10.2337/diabetes.52.6.1319 · Cited by 305 · see citing works
Schrauwen-Hinderling, V. B., Kooi, M. E., Hesselink, M. K. C., Jeneson, J. A. L., Backes, W. H., van Echteld, C. J. A., van Engelshoven, J. M. A., Mensink, M., & Schrauwen, P. (2007) · Diabetologia · doi:10.1007/s00125-006-0475-1 · Cited by 304 · see citing works
Vaag, A., Henriksen, J. E., & Beck-Nielsen, H. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115656 · Cited by 299 · cited 2× · see citing works
“Consistently, impaired insulin activation of phosphatidylinositol 3-kinase and glycogen synthase has been demonstrated in muscle of patients with type 2 diabetes [2,10], their first-degree relatives [ 6 ] and diabetic myotubes [7, 8, 11, 12].”
BackgroundMethodRichardson, D. K., Kashyap, S., Bajaj, M., Cusi, K., Mandarino, S. J., Finlayson, J., DeFronzo, R. A., Jenkinson, C. P., & Mandarino, L. J. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m408985200 · Cited by 259 · see citing works
Gene Expression Profile in Skeletal Muscle of Type 2 Diabetes and the Effect of Insulin Treatment
Sreekumar, R., Halvatsiotis, P., Schimke, J. C., & Nair, K. S. (2002) · Diabetes · doi:10.2337/diabetes.51.6.1913 · Cited by 257 · see citing works
Muscle Mitochondrial ATP Synthesis and Glucose Transport/Phosphorylation in Type 2 Diabetes
Szendroedi, J., Schmid, A. I., Chmelik, M., Toth, C., Brehm, A., Krssak, M., Nowotny, P., Wolzt, M., Waldhausl, W., & Roden, M. (2007) · PLoS Medicine · doi:10.1371/journal.pmed.0040154 · Cited by 243 · see citing works
Højlund, K., Wrzesinski, K., Larsen, P. M., Fey, S. J., Roepstorff, P., Handberg, A., Dela, F., Vinten, J., McCormack, J. G., Reynet, C., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212881200 · Cited by 221 · see citing works
Skov, V., Glintborg, D., Knudsen, S., Jensen, T. E., Kruse, T. A., Tan, Q., Brusgaard, K., Beck‐Nielsen, H., & Højlund, K. (2007) · Diabetes · doi:10.2337/db07-0275 · Cited by 202 · see citing works
Rome, S., Clément, K., Rabasa-Lhoret, R., Loizon, E., Poitou, C., Barsh, G. S., Riou, J.-P., Laville, M., & Vidal, H. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300293200 · Cited by 187 · see citing works
Nair, K. S., Bigelow, M. L., Asmann, Y. W., Chow, L. S., Coenen-Schimke, J. M., Klaus, K. A., Guo, Z.-K., Sreekumar, R., & Irving, B. A. (2008) · Diabetes · doi:10.2337/db07-1556 · Cited by 187 · see citing works
Reduced Lipid Oxidation in Skeletal Muscle From Type 2 Diabetic Subjects May Be of Genetic Origin
Gaster, M., Rustan, A. C., Aas, V., & Beck-Nielsen, H. (2004) · Diabetes · doi:10.2337/diabetes.53.3.542 · Cited by 176 · see citing works
The Diabetic Phenotype Is Conserved in Myotubes Established From Diabetic Subjects
Gaster, M., Petersen, I. M., Højlund, K., Poulsen, P., & Beck‐Nielsen, H. (2002) · Diabetes · doi:10.2337/diabetes.51.4.921 · Cited by 150 · see citing works
A cellular model system of differentiated human myotubes
Gaster, M., Kristensen, S., Beck‐Nielsen, H., & Schrøder, H. D. (2001) · Apmis · doi:10.1034/j.1600-0463.2001.d01-140.x · Cited by 140 · see citing works
Henry, R. R., Ciaraldi, T. P., Abrams-Carter, L., Mudaliar, S. R., Park, K., & Nikoulina, S. E. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118906 · Cited by 129 · see citing works
Karakelides, H., Asmann, Y. W., Bigelow, M. L., Short, K. R., Dhatariya, K., Coenen-Schimke, J., Kahl, J., Mukhopadhyay, D., & Nair, K. S. (2007) · Diabetes · doi:10.2337/db07-0378 · Cited by 123 · see citing works
Højlund, K., Stæhr, P., Hansen, B. F., Green, K. A., Hardie, D. G., Richter, E. A., Beck‐Nielsen, H., & Wojtaszewski, J. F. P. (2003) · Diabetes · doi:10.2337/diabetes.52.6.1393 · Cited by 122 · see citing works
Beck‐Nielsen, H., ALFORD, F. P., Levin, K., & Schroeder, H. D. (2001) · Diabetologia · doi:10.1007/s001250100545 · Cited by 104 · see citing works
Ørtenblad, N., Mogensen, M., Petersen, I., Højlund, K., Levin, K., Sahlin, K., Beck-Nielsen, H., & Gaster, M. (2005) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2005.04.001 · Cited by 101 · see citing works
Self-citationHøjlund, K., Wildner‐Christensen, M., Eshøj, O., Skjærbæk, C. B., Holst, J. J., Koldkjær, O. G., Jensen, D. M., & Beck‐Nielsen, H. (2001) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.2001.280.1.e50 · Cited by 89 · see citing works
The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle
Wu, X., Wang, J., Cui, X., Maianu, L., Rhees, B. K., Rosinski, J., So, W. V., Willi, S. M., Osier, M. V., Hill, H. S., et al. (2007) · Endocrine · doi:10.1007/s12020-007-0007-x · Cited by 79 · see citing works
Beck‐Nielsen, H., Vaag, A. A., Poulsen, P., & Gaster, M. (2003) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/s1521-690x(03)00041-1 · Cited by 77 · see citing works
Nikoulina, S. E., Ciaraldi, T. P., Carter, L., Mudaliar, S. R. D., Park, K. S., & Henry, R. R. (2001) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.86.9.7872 · Cited by 65 · see citing works
Publisher, B. S. P. B. S. (2006) · Current Diabetes Reviews · doi:10.2174/1573399810602040375 · Cited by 59 · see citing works
Hansen, L., Gaster, M., Oakeley, E. J., Brusgaard, K., Nielsen, E.-M. D., Beck‐Nielsen, H., Pedersen, O. B., & Hemmings, B. A. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.08.146 · Cited by 38 · see citing works
Gaster, M., & Beck-Nielsen, H. (2004) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2004.05.006 · Cited by 38 · see citing works
Effect of insulin on protein phosphatase 2A expression in muscle in type 2 diabetes
Højlund, K., Poulsen, M. K., Stæhr, P., Brusgaard, K., & Beck‐Nielsen, H. (2002) · European Journal of Clinical Investigation · doi:10.1046/j.1365-2362.2002.01098.x · Cited by 37 · see citing works
Correspondence analysis of microarray time-course data in case–control design
Tan, Q., Brusgaard, K., Kruse, T. A., Oakeley, E. J., Hemmings, B. A., Beck‐Nielsen, H., Hansen, L., & Gaster, M. (2004) · Journal of Biomedical Informatics · doi:10.1016/j.jbi.2004.06.001 · Cited by 23 · see citing works
Gaster, M., Ottosen, P. D., Vach, W., CHRISTIANSEN, H., Stæhr, P., Beck‐Nielsen, H., & Schrøder, H. D. (2003) · Apmis · doi:10.1034/j.1600-0463.2003.1110207.x · Cited by 15 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Comprehensive review on lactate metabolism in human health
Adeva‐Andany, M. M., López-Ojén, M., Funcasta-Calderón, R., Ameneiros-Rodríguez, E., Donapetry, C., Vila-Altesor, M., & Rodríguez-Seijas, J. (2014) · Mitochondrion · doi:10.1016/j.mito.2014.05.007 · Cited by 614 · see citing works
The role of mitochondria in the pathogenesis of type 2 diabetes
Patti, M.-E., & Corvera, S. (2010) · Endocrine Reviews · doi:10.1210/er.2009-0027 · Cited by 551 · see citing works
Väremo, L., Scheele, C. C., Broholm, C., Mardinoğlu, A., Kampf, C., Asplund, A., Nookaew, I., Uhlén, M., Pedersen, B. K., & Nielsen, J. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.04.010 · Cited by 116 · see citing works
Yan, J., Feng, Z., Liu, J., Shen, W., Wang, Y., Wertz, K., Weber, P., Long, J., & Liu, J. (2012) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2011.03.014 · Cited by 110 · see citing works
Elbein, S. C., Kern, P. A., Rasouli, N., Yao‐Borengasser, A., Sharma, N. K., & Das, S. K. (2011) · Diabetes · doi:10.2337/db10-1270 · Cited by 107 · see citing works
Yamaguchi, T., Suzuki, T., Arai, H., Tanabe, S., & Atomi, Y. (2009) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00050.2009 · Cited by 99 · see citing works
Yang, C., Aye, C. C., Li, X., Díaz‐Ramos, A., Antonio Zorzano, A., & Mora, S. (2012) · Bioscience Reports · doi:10.1042/bsr20120034 · Cited by 58 · see citing works
Zhao, C., Mao, J., Ai, J., Shenwu, M., Shi, T., Zhang, D., Wang, X., Wang, Y., & Deng, Y. (2013) · BMC Medical Genomics · doi:10.1186/1755-8794-6-s1-s12 · Cited by 55 · see citing works
Gaster, M., Nehlin, J. O., & Minet, A. D. (2012) · Archives of Physiology and Biochemistry · doi:10.3109/13813455.2012.656653 · Cited by 53 · see citing works
Brown, A. E., Palsgaard, J., Borup, R., Avery, P. J., Gunn, D. A., MEYTS, P. D., Yeaman, S. J., & Walker, M. (2014) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00115.2014 · Cited by 52 · see citing works
Nehlin, J. O., Just, M., Rustan, A. C., & Gaster, M. (2011) · Biogerontology · doi:10.1007/s10522-011-9336-5 · Cited by 48 · see citing works
Reduced TCA flux in diabetic myotubes: A governing influence on the diabetic phenotype?
Gaster, M. (2009) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2009.07.064 · Cited by 44 · see citing works
Minet, A. D., & Gaster, M. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.09.115 · Cited by 42 · see citing works
Mirza, Z., Kamal, M. A., buzenadah, A., Al-Qahtani, M., & Karim, S. (2014) · CNS & Neurological Disorders - Drug Targets · doi:10.2174/18715273113126660154 · Cited by 42 · see citing works
Bimodal distribution of RNA expression levels in human skeletal muscle tissue
Mason, C. C., Hanson, R. L., Ossowski, V., Bian, L., Baier, L. J., Krakoff, J. A., & Bogardus, C. (2011) · BMC Genomics · doi:10.1186/1471-2164-12-98 · Cited by 41 · see citing works
Thingholm, T. E., Bak, S. Y., Beck‐Nielsen, H., Jensen, O. N., & Gaster, M. (2011) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m110.006650 · Cited by 41 · see citing works
Intact Primary Mitochondrial Function in Myotubes Established from Women with PCOS
Eriksen, M. B., Minet, A. D., Glintborg, D., & Gaster, M. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-0278 · Cited by 35 · see citing works
Kitzmann, M., Lantier, L., Hébrard, S., Mercier, J. G., Foretz, M., & Aguer, C. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2010.12.007 · Cited by 33 · see citing works
Mukaida, S., Evans, B. A., Bengtsson, T., Hutchinson, D. S., & Sato, M. (2016) · Pharmacological Research · doi:10.1016/j.phrs.2016.12.022 · Cited by 33 · see citing works
Adipose tissue–specific ablation of Ces1d causes metabolic dysregulation in mice
Li, G., Li, X., Yang, L., Wang, S., Dai, Y., Fekry, B., Veillon, L. J., Tan, L., Berdeaux, R., Eckel‐Mahan, K. L., et al. (2022) · Life Science Alliance · doi:10.26508/lsa.202101209 · Cited by 30 · see citing works
Insulin Resistance Is Not Conserved in Myotubes Established from Women with PCOS
Eriksen, M. B., Pørneki, A. D., Skov, V., Burns, J. S., Beck‐Nielsen, H., Glintborg, D., & Gaster, M. (2010) · PLoS ONE · doi:10.1371/journal.pone.0014469 · Cited by 28 · see citing works
Targeting the Metabolic Paradigms in Cancer and Diabetes
Bosso, M., Haddad, D., Madhoun, A. S. A., & Al‐Mulla, F. (2024) · Biomedicines · doi:10.3390/biomedicines12010211 · Cited by 26 · see citing works
Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice
Singh, R. K., Fullerton, M. D., Vine, D. F., & Bakovic, M. (2012) · Journal of Lipid Research · doi:10.1194/jlr.m021881 · Cited by 27 · see citing works
Minet, A. D., & Gaster, M. (2012) · Biogerontology · doi:10.1007/s10522-012-9372-9 · Cited by 25 · see citing works
Identification of secreted proteins associated with obesity and type 2 diabetes in Psammomys obesus
Bolton, K. A., Segal, D., McMillan, J. S., Sanigorski, A. J., Collier, G. R., & Walder, K. R. (2009) · International Journal of Obesity · doi:10.1038/ijo.2009.148 · Cited by 23 · see citing works
Wu, Y.-L., Lin, H., Li, H.-F., Don, M., King, P.-C., & Chen, H.-H. (2022) · Cells · doi:10.3390/cells11061022 · Cited by 22 · see citing works
Brands, M., Hoeks, J., Sauerwein, H. P., Ackermans, M. T., Ouwens, M., Lammers, N. M., van der Plas, M. N., Schrauwen, P., Groen, A. K., & Serlie, M. J. (2011) · Metabolism · doi:10.1016/j.metabol.2011.02.006 · Cited by 19 · see citing works
Insulin immuno-neutralization in fed chickens: effects on liver and muscle transcriptome
Simon, J., Milenković, D., Godet, E., Cabau, C., Collin, A., Métayer, S., Rideau, N., Tesseraud, S., Derouet, M., Crochet, S., et al. (2012) · Physiological Genomics · doi:10.1152/physiolgenomics.00057.2011 · Cited by 17 · see citing works
The Ups and Downs of Insulin Resistance and Type 2 Diabetes: Lessons from Genomic Analyses in Humans
Sales, V., & Patti, M. (2012) · Current Cardiovascular Risk Reports · doi:10.1007/s12170-012-0283-8 · Cited by 17 · see citing works
Yang, Y., Yao, S., Ding, J., Chen, W., & Guo, Y. (2020) · Diabetes & Metabolism Journal · doi:10.4093/dmj.2019.0204 · Cited by 16 · see citing works
Reduced TCA Flux in Diabetic Myotubes: Determined by Single Defects?
Gaster, M. (2012) · Biochemistry Research International · doi:10.1155/2012/716056 · Cited by 16 · see citing works
Mathur, S. K., Jain, P., & Mathur, P. (2011) · Journal of Obesity · doi:10.1155/2011/587495 · Cited by 15 · see citing works
Minet, A. D., & Gaster, M. (2011) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2011.04.028 · Cited by 14 · see citing works
Adrenoceptor regulation of the mechanistic target of rapamycin in muscle and adipose tissue
Chia, L. Y., Evans, B. A., Mukaida, S., Bengtsson, T., Hutchinson, D. S., & Sato, M. (2019) · British Journal of Pharmacology · doi:10.1111/bph.14616 · Cited by 14 · see citing works
Mitochondrial mass is inversely correlated to complete lipid oxidation in human myotubes
Gaster, M. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.12.102 · Cited by 11 · see citing works
Abdallah, B. M., Beck‐Nielsen, H., & Gaster, M. (2013) · Frontiers in Endocrinology · doi:10.3389/fendo.2013.00045 · Cited by 11 · see citing works
Malate dehydrogenase as a multi-purpose target for drug discovery
Fermaintt, C. S., & Wacker, S. (2024) · Essays in Biochemistry · doi:10.1042/ebc20230081 · Cited by 10 · see citing works
Gaster, M. (2018) · Apmis · doi:10.1111/apm.12908 · Cited by 10 · see citing works
Skeletal muscle tissue transcriptome differences in lean and obese female beagle dogs
Grant, R. W., Boler, B. M. V., Ridge, T. K., Graves, T. K., & Swanson, K. S. (2013) · Animal Genetics · doi:10.1111/age.12035 · Cited by 10 · see citing works
Shao, K., Shen, L., Li, H., Huang, S., & Zhang, Y. (2017) · Journal of Cellular Physiology · doi:10.1002/jcp.26174 · Cited by 10 · see citing works
Matrix metalloproteinase 14 modulates diabetes and Alzheimer’s disease cross-talk: a meta-analysis
Cheng, J., Liu, H.-P., Lee, C.-C., Chen, M.-Y., Lin, W., & Tsai, F. (2017) · Neurological Sciences · doi:10.1007/s10072-017-3166-4 · Cited by 11 · see citing works
Role of Mitochondrial Function in Insulin Resistance
Brands, M., Verhoeven, A. J., & Serlie, M. J. M. (2011) · Advances in experimental medicine and biology · doi:10.1007/978-94-007-2869-1_9 · Cited by 9 · see citing works
Minet, A. D., & Gaster, M. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-1384 · Cited by 9 · see citing works
Tissue Non-Specific Genes and Pathways Associated with Diabetes: An Expression Meta-Analysis
Mei, H., Li, L., Liu, S., Jiang, F., Griswold, M. E., & Mosley, T. H. (2017) · Genes · doi:10.3390/genes8010044 · Cited by 9 · see citing works
Environmental pollution and diabetes mellitus
Sikaily, A. E., & Helal, M. (2021) · World Journal of Meta-Analysis · doi:10.13105/wjma.v9.i3.234 · Cited by 7 · see citing works
DEPP Deficiency Contributes to Browning of White Adipose Tissue
Guo, F., Zhu, Y., Han, Y., Feng, X., Pan, Z., He, Y., Li, Y., & Jin, L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23126563 · Cited by 6 · see citing works
Aye, C. C., Hammond, D. E., Rodríguez‐Cuenca, S., Doherty, M. K., Whitfield, P. D., Phelan, M. M., Yang, C., Pérez‐Pérez, R., Li, X., Díaz‐Ramos, A., et al. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24043399 · Cited by 4 · see citing works
Lange, M., Nebel, D., & Villmann, T. (2014) · Advances in intelligent systems and computing · doi:10.1007/978-3-319-07695-9_25 · Cited by 4 · see citing works
Transcriptomics und Typ-2-Diabetes
Herder, C., Roden, M., Carstensen, M., Illig, T., & Prokisch, H. (2012) · Der Diabetologe · doi:10.1007/s11428-011-0777-x · Cited by 3 · see citing works
Park, A., Kang, B. E., Jin, E., Kim, H. J., Lee, C.-W., Suh, J. M., Ryu, D., & Nam, S. (2025) · Genes & Genomics · doi:10.1007/s13258-025-01727-7 · Cited by 2 · see citing works
Ding, Z., Cao, L., Jin, R., & Li, R. (2023) · Clinical and Experimental Obstetrics & Gynecology · doi:10.31083/j.ceog5004073 · Cited by 1 · see citing works
Saxena, A. K., Mathur, N., Chaudhary, U., Raj, U., Rai, S., & Mathur, S. K. (2022) · Journal of Applied Biology & Biotechnology · doi:10.7324/jabb.2023.110116 · Cited by 1 · see citing works
Oduaran, O. H. (2015) · University of the Witwatersrand, Johannesburg Institutional Repository on DSpace (University of the Witwatersrand, Johannesburg) · Cited by 1 · see citing works
Hjelle, L. (2011) · NORA - Norwegian Open Research Archives
The effect of overfeeding and obesity on canine adipose tissue and skeletal muscle transcriptomes
Grant, R. W. (2011) · Illinois Digital Environment for Access to Learning and Scholarship (University of Illinois at Urbana-Champaign)
Current literature in diabetes
(2009) · Diabetes/Metabolism Research and Reviews · doi:10.1002/dmrr.952
- Authors
- Frederiksen, C. M., Højlund, K., Hansen, L., Oakeley, E. J., Hemmings, B., Abdallah, B. M., Brusgaard, K., Beck-Nielsen, H., & Gaster, M.
- Affiliations
- Odense University Hospital (DK)
- Friedrich Miescher Institute (CH)
- University of Southern Denmark (DK)
- Corresponding author
- Højlund, K.
- Venue
- Diabetologia
- Published
- 2008-08-22
- Volume
- 51
- Issue
- 11
- Pages
- 2068-2077
- ISSN
- 0012-186X (ISSN-L)
- 1432-0428
- OpenAlex
- W2029631235
- Cited by
- 57
- References
- 44
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 4 · 2013: 3 · 2012: 9
- FWCI
- 2.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Diabetologia (publishedVersion)
- PubMed (publishedVersion)
- University of Southern Denmark Research Portal (University of Southern Denmark) (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- type 2 diabetes, myotubes, skeletal muscle, insulin resistance, mitochondrial biogenesis, oxidative phosphorylation, PGC-1α, NRF1, gene expression profiling, lipid oxidation, glucose oxidation, glycogen synthesis, obesity
- MeSH terms
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Diabetes Mellitus, Type 2, Heat-Shock Proteins, Humans, Middle Aged, Obesity, Oxidative Phosphorylation, RNA, Transcription Factors, Transcription, Genetic, Body Mass Index, Muscle, Skeletal, Muscle Fibers, Skeletal, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Nuclear Respiratory Factor 1
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
