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The isoprostanes—25 years later
Open accessAbstract
Isoprostanes (IsoPs) are prostaglandin-like molecules generated independent of the cyclooxygenase (COX) by the free radical-induced peroxidation of arachidonic acid. The first isoprostane species discovered were isomeric to prostaglandin F2α and were thus termed F2-IsoPs. Since the initial discovery of the F2-IsoPs, IsoPs with differing ring structures have been identified as well as IsoPs from different polyunsaturated fatty acids, including eicosapentaenoic acid and docosahexanenoic acid. The discovery of these molecules in vivo in humans has been a major contribution to the field of lipid oxidation and free radical research over the course of the past 25 years. These molecules have been determined to be both biomarkers and mediators of oxidative stress in numerous disease settings. This review focuses on recent developments in the field with an emphasis on clinical research. Special focus is given to the use of IsoPs as biomarkers in obesity, ischemia-reperfusion injury, the central nervous system, cancer, and genetic disorders. Additionally, attention is paid to diet and lifestyle factors that can affect endogenous levels of IsoPs. This article is part of a Special Issue entitled "Oxygenated metabolism of PUFA: analysis and biological relevance."
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“Isoprostanes are specific prostaglandin-like molecules formed by the non-enzymatic ROS-mediated peroxidation of PUFAs, involving for example arachidonic acid (Milne et al. 2015).”
From Reactive Oxygen Species, Toxicity, Oxidative Stress, and Antioxidants: Chronic Diseases and Aging (2023) · cites this paper as Milne et al. 2015
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“Increased amounts of F2-IsoPs are detected in cardiovascular diseases and other clinical conditions (Milne et al. 2015).”
From Reactive Oxygen Species, Toxicity, Oxidative Stress, and Antioxidants: Chronic Diseases and Aging (2023) · cites this paper as Milne et al. 2015
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Morrow, J. D., Hill, K. E., Burk, R. F., Nammour, T., Badr, K. F., & Roberts, L. J. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.23.9383 · Cited by 1,898
Self-citationKeaney, J. F., Larson, M. G., Vasan, R. S., Wilson, P. W. F., Lipinska, I., Corey, D., Massaro, J. M., Sutherland, P., Vita, J. A., & Benjamin, E. J. (2003) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000058402.34138.11 · Cited by 1,304
Lipid peroxidation: Physiological levels and dual biological effects
Niki, E. (2009) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2009.05.032 · Cited by 1,032
Pou, K. M., Massaro, J. M., Hoffmann, U., Vasan, R. S., Maurovich‐Horvat, P., Larson, M. G., Keaney, J. F., Meigs, J. B., Lipinska, I., Kathiresan, S., et al. (2007) · Circulation · doi:10.1161/circulationaha.107.710509 · Cited by 915
Biological markers of oxidative stress: Applications to cardiovascular research and practice
Ho, E. C., Galougahi, K. K., Liu, C., Bhindi, R., & Figtree, G. A. (2013) · Redox Biology · doi:10.1016/j.redox.2013.07.006 · Cited by 835
Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans
Vincent, H. K., & Taylor, A. G. (2005) · International Journal of Obesity · doi:10.1038/sj.ijo.0803177 · Cited by 752
Kadiiska, M. B., Gladen, B. C., Baird, D. D., Germolec, D. R., Graham, L., Parker, C. E., Nyska, A., Wachsman, J. T., Ames, B. N., Basu, S., et al. (2004) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.09.017 · Cited by 699
Self-citationMorrow, J. D., Harris, T. M., & Roberts, L. J. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90002-q · Cited by 479
Self-citationMorrow, J. D. (2005) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000152605.64964.c0 · Cited by 449
Oxidative stress and potential interventions to reduce oxidative stress in overweight and obesity
Vincent, H. K., Innes, K. E., & Vincent, K. R. (2007) · Diabetes Obesity and Metabolism · doi:10.1111/j.1463-1326.2007.00692.x · Cited by 443
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Research works printed but not linked: 1
- Dai, 2009, Oxidative stress, obesity, and breast cancer risk: results from the Shanghai Women's health study, J. Clin. Oncol., 27, 2482, 10.1200/JCO.2008.19.7970 · DOI
Cited by
Sorted by citations
Items 301–335 of 335
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Picolli, T. (2022) · Digital Archive @ GSU · doi:10.57709/11198439
Pais, R. T., Sousa, A. C. A., & Pastorinho, M. R. (2018) · Environmental Pollution · doi:10.1016/j.envpol.2018.09.033
Oxidative Stress Signatures in Human Stem Cell-Derived Neurons
Neely, M. D., & Bowman, A. B. (2019) · Neuromethods · doi:10.1007/978-1-4939-9228-7_3
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Eierhoff, T., Karamperidis, L., & Oberhuber, A. (2020) · Gefässchirurgie · doi:10.1007/s00772-020-00645-0
Preparation and Chiral HPLC Separation of the Enantiomeric Forms of Natural Prostaglandins
Enesei, M., Takács, L., Tormási, E., Rácz, A. T., Róka, Z., Kádár, M., & Kardos, Z. (2020) · Chemistry · doi:10.3390/chemistry2030047
Nogueira, M. S. (2020) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.9.2020.tde-20122021-112905
Arndt, T. (2017) · Springer eBooks · doi:10.1007/978-3-662-49054-9_3699-1
Arndt, T. (2017) · Springer eBooks · doi:10.1007/978-3-662-49054-9_1638-1
Arndt, T. (2019) · Springer Reference Medizin · doi:10.1007/978-3-662-48986-4_1638
Arndt, T. (2019) · Springer Reference Medizin · doi:10.1007/978-3-662-48986-4_3699
Petrone-García, V. M., López‐Arellano, R., Rodríguez‐Patiño, G., Rodríguez, M. A. C., Hernández‐Patlan, D., Solís-Cruz, B., Hernández-Velasco, X., Alba-Hurtado, F., Vuong, C. N., Castellanos-Huerta, I., et al. (2021) · Research Square · doi:10.21203/rs.3.rs-289590/v1
Clinical relevance of oxidative stress biomarkers in cardiovascular prevention
Pimentel, M. V. D. M. B. (2022) · doi:10.11606/d.9.2022.tde-15022023-135127
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Hassan, S., Eid, M., Hassan, A., & Hayek, S. E. (2023) · Nutritional neurosciences · doi:10.1007/978-981-99-1513-2_8
Kumar, S., Acharya, N., Acharya, S., Shukla, S., Mohammad, S., & Sinha, P. (2023) · Journal of South Asian Federation of Obstetrics and Gynaecology · doi:10.5005/jp-journals-10006-2286
Biologically Active Lipids in Vascular Biology
Upchurch, C. M., & Leitinger, N. (2024) · Learning materials in biosciences · doi:10.1007/978-3-031-64591-4_9
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Teyin, G., & NİZAMLIOĞLU, H. F. (2025) · Journal of Aquatic Food Product Technology · doi:10.1080/10498850.2025.2599807
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Voros, C., Chatzinikolaou, F., Papadimas, G. K., Karpouzos, A., Koulakmanidis, A.-M., Athanasiou, D., Bananis, K., Athanasiou, A., Athanasiou, A. V., Τsimpoukelis, C., et al. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27114710
Ковальчук, А., Zinych, О. В., Shyshkan-Shyshova, К. О., Кушнарьова, Н. М., Prybyla, О. В., & Шупрович, А. (2026) · INTERNATIONAL JOURNAL OF ENDOCRINOLOGY (Ukraine) · doi:10.22141/2224-0721.22.4.2026.1722
Tsikas, D., Habler, K., & Ückert, P. S. (2026) · Journal of Clinical Medicine · doi:10.3390/jcm15145518
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Lopez-Garcia, M., Ramírez-Hernández, A., de la Rosa-Cordero, A. A., Vergara-Tamayo, E. A., Bracho-Velasco, M., Aguilar-Reyes, O. S., & O’Neill-Suárez, S. (2026) · IntechOpen eBooks · doi:10.5772/intechopen.1016699
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Masini, G., Chiusolo, S., Alberti, M., Liga, R., Galeotti, G. G., Lattanzi, F., Morganti, R., Gargani, L., Carnevale, R., Violi, F., et al. (2026) · IJC Heart & Vasculature · doi:10.1016/j.ijcha.2026.101976
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Mittal, R., Alberti, A., Shivakumar, R., Selvan, L., Gupta, V., Aggarwal, A., Patel, V., Blaschke, C. E., Alipour, F., & Hirani, K. (2026) · Antioxidants · doi:10.3390/antiox15081044
Shen, C., Newman, J. W., Snodgrass, I. F., Caruso, D. J., Chyu, M., Neugebauer, V., & Watkins, B. A. (2026) · Frontiers in Medicine · doi:10.3389/fmed.2026.1907924
Atalan, S. B., & Ayaz, A. (2026) · Nutrients · doi:10.3390/nu18172836
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Suresh, D. R., Annam, V., Suligavi, M., Pujari, S., Shankar, P., & Sreedharan, P. (2026) · Indian Journal of Medical Biochemistry · doi:10.5005/jp-journals-10054-0360
Woźniak, Ł., Antonowicz, B., Mierzejewska, Ż. A., Kosicka, E., Lechien, J. R., Vaira, L. A., & Borys, J. (2026) · Antioxidants · doi:10.3390/antiox15091174
Arwari, J. M., Strakovsky, R. S., Pathak, K. V., Lovell, B., Pirrotte, P., Milne, G. L., Chandler, M., Schantz, S. L., & Eick, S. M. (2026) · ACS Nutrition Science · doi:10.1021/acsnutrsci.6c00074
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Куракин, Г. Ф. (2026) · Biochemistry (Moscow) · doi:10.1134/s0006297926601851
Metadata
- Authors
- Milne, G. L., Dai, Q., & Roberts, L. J.
- Affiliations
- Vanderbilt University (US)
- Corresponding author
- Roberts, L. Jackson
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
- Publisher
- Elsevier BV
- Published
- 2015-04
- Volume
- 1851
- Issue
- 4
- Pages
- 433-445
- ISSN
- 1388-1981 (ISSN-L)
- PMC
- PMC5404383
- OpenAlex
- W2059867409
- Cited by
- 335
- References
- 238
- Citations by year
- 2026: 20 · 2025: 20 · 2024: 17 · 2023: 26 · 2022: 30 · 2021: 37 · 2020: 39 · 2019: 37 · 2018: 31 · 2017: 31 · 2016: 34 · 2015: 13
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- GM15431
- ES000267
- CA106591
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
- Keywords
- isoprostanes, lipid peroxidation, arachidonic acid, free radicals, oxidative stress, biomarkers, F2-isoprostanes, eicosapentaenoic acid, docosahexaenoic acid, obesity, ischemia-reperfusion injury, central nervous system, cancer, genetic disorders, diet and lifestyle, polyunsaturated fatty acids
- MeSH terms
- Animals, Disease, Humans, Structure-Activity Relationship, Lipid Peroxidation, Molecular Structure, Signal Transduction, Biomarkers, Oxidative Stress, Isoprostanes
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