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Cited by 57Fatty Acid Research and HealthLipid metabolism and biosynthesisMuscle Physiology and Disorders

Myogenic differentiation and lipid-raft composition of l6 skeletal muscle cells are modulated by pufas

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Briolay, Anne, Jaafar, Rami, Nemoz, Georges, Bessueille, Laurence·2013·Biochimica et Biophysica Acta (BBA) - Biomembranes
DOI 10.1016/j.bbamem.2012.10.006
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Abstract

Lipid composition and fatty acid analysis of the major classes of membrane phospholipids were determined during myogenic differentiation of L6 skeletal muscle cells. The cholesterol to glycerophospholipids ratio decreased during differentiation, both in total (TM) and detergent-resistant membranes (DRM). Analyses of the membrane lipids showed that differentiation had a major impact on the molecular composition of glycerophospholipids. A significant decrease in the concentration of saturated fatty acids was detected in glycerophospholipid classes, and to a lesser extent in sphingolipids, while the concentration of 16:1n-7, 18:1n-7 and 18:1n-9 increased. At the same time, the concentration of long polyunsaturated fatty acid chains decreased in TM and DRM glycerophospholipids, resulting in a lower saturated to unsaturated fatty acid ratio in myotubes as compared to myoblasts. Interestingly, the observed n-3/n-6 ratio was lower in differentiated cell membranes. PUFA supplementation of L6 cells led to an increase in myogenic differentiation correlated to an incorporation of added PUFAs in TM and DRM glycerophospholipids. As expected after n-3 PUFA supplementation, the n-3/n-6 ratio was clearly increased in TM and, surprisingly, this was also the case in isolated DRM. n-3 and n-6 PUFAs significantly and time-dependently increased the phosphorylation of kinase p70S6K1 during myogenic differentiation, revealing the activation of the upstream kinase mTORC1, a major regulator of cell cycle and protein translation. In contrast, PUFAs did not affect the phosphorylation of the kinase Akt, another pivotal regulator of cell metabolism. These results suggest that PUFA supplementation modified the membrane lipid composition and affected the differentiation of L6 cells.

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Crossref · 10.1016/j.bbamem.2012.10.006

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  • The potential positive effects of n-3 PUFAs on myogenesis may relate to their capacity to alter cell membrane lipid composition, changing the profile of membrane-bound proteins of lipid rafts.

    “The potential positive effects of n-3 PUFA’s on myogenesis may relate to their capacity to alter the cell membrane lipid composition, which in turn, changes the profile of membrane bound proteins of lipid rafts [79,80].”

    From Potential Roles of n-3 PUFAs during Skeletal Muscle Growth and Regeneration (2018) · cites this paper as [79]

  • EPA and DHA induce similar increases in p70S6K1 phosphorylation without leucine stimulation during differentiation.

    “Others have also shown EPA and DHA to induce similar increases in p70S6K1 phosphorylation without leucine stimulation during differentiation [79].”

    From Potential Roles of n-3 PUFAs during Skeletal Muscle Growth and Regeneration (2018) · cites this paper as [79]

  • Activation of p70S6K1 following n-3 PUFA treatment is not associated with an increase in Akt activity, an upstream effector of mTOR implicated in skeletal muscle hypertrophy.

    “The activation of p70S6K1 following n-3 PUFA treatment in this case is not associated with an increase in Akt activity, which is an upstream effector of mTOR that is implicated in skeletal muscle hypertrophy [79,123].”

    From Potential Roles of n-3 PUFAs during Skeletal Muscle Growth and Regeneration (2018) · cites this paper as [79]

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Authors
Briolay, A., Jaafar, R., Nemoz, G., & Bessueille, L.
Affiliations
Lyon 1 Université (FR)
Corresponding author
Bessueille, Laurence
ORCID
Bessueille, Laurence
Venue
Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher
Elsevier BV
Published
2013-02
Volume
1828
Issue
2
Pages
602-613
ISSN
0005-2736 (ISSN-L)
DOI
10.1016/j.bbamem.2012.10.006
OpenAlex
W2067980390
Cited by
57
References
63
Citations by year
2026: 3 · 2025: 2 · 2024: 5 · 2023: 3 · 2022: 2 · 2021: 5 · 2020: 5 · 2019: 3 · 2018: 10 · 2017: 5 · 2016: 2 · 2015: 4 · 2014: 7 · 2013: 1
FWCI
3.57
Type
journal article
Language
en
Open access
Licence
Locations
  • Biochimica et Biophysica Acta (BBA) - Biomembranes (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
myogenesis, L6 myoblasts, polyunsaturated fatty acids, lipid rafts, detergent-resistant membranes, membrane phospholipids, glycerophospholipids, cholesterol/phospholipid ratio, n-6/n-3 ratio, mTORC1, Akt, skeletal muscle
MeSH terms
Animals, Biophysics, Cell Differentiation, Cholesterol, Creatine Kinase, Detergents, Fatty Acids, Fatty Acids, Unsaturated, Lipids, Microscopy, Fluorescence, Phosphorylation, Time Factors, Signal Transduction, Muscle, Skeletal, Glycerophospholipids, Membrane Microdomains, Muscle Development, Proto-Oncogene Proteins c-akt, Rats

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