Matrigel Basement Membrane Matrix influences expression of microRNAs in cancer cell lines
Abstract
Matrigel is a medium rich in extracellular matrix (ECM) components used for three-dimensional cell culture and is known to alter cellular phenotypes and gene expression. microRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and have roles in cancer. While miRNA profiles of numerous cell lines cultured on plastic have been reported, the influence of Matrigel-based culture on cancer cell miRNA expression is largely unknown. This study investigated the influence of Matrigel on the expression of miRNAs that might facilitate ECM-associated cancer cell growth. We performed miRNA profiling by microarray using two colon cancer cell lines (SW480 and SW620), identifying significant differential expression of miRNAs between cells cultured in Matrigel and on plastic. Many of these miRNAs have previously been implicated in cancer-related processes. A common Matrigel-induced miRNA signature comprised of up-regulated miR-1290 and miR-210 and down-regulated miR-29b and miR-32 was identified using RT-qPCR across five epithelial cancer cell lines (SW480, SW620, HT-29, A549 and MDA-MB-231). Experimental modulation of these miRNAs altered expression of their known target mRNAs involved in cell adhesion, proliferation and invasion, in colon cancer cell lines. Furthermore, ITGA5 was identified as a novel putative target of miR-32 that may facilitate cancer cell interactions with the ECM. We propose that culture of cancer cell lines in Matrigel more accurately recapitulates miRNA expression and function in cancer than culture on plastic and thus is a valuable approach to the in vitro study of miRNAs.
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Various cancer cell lines grown in 2D and 3D culture often display different gene expression profiles, especially many genes that play a role in proliferation, angiogenesis, migration, invasion, and chemosensitivity.
“Various cancer cell lines grown in 2D and 3D culture often display different gene expression profiles, especially many genes that play a role in proliferation, angiogenesis, migration, invasion, and chemosensitivity. 17,40”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [40]
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- Authors
- Price, K. J., Tsykin, A., Giles, K. M., Sladic, R. T., Epis, M. R., Ganss, R., Goodall, G. J., & Leedman, P. J.
- Affiliations
- The University of Western Australia (AU)
- South Australia Pathology (AU)
- Corresponding author
- Leedman, Peter J.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2012-10
- Volume
- 427
- Issue
- 2
- Pages
- 343-348
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2059490699
- Cited by
- 49
- References
- 50
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 8 · 2019: 6 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 1 · 2014: 6 · 2013: 2
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- UWA Profiles and Research Repository (University of Western Australia) (submittedVersion)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
- Funders
- University of Western Australia
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- Matrigel, extracellular matrix, microRNA expression, colon cancer cell lines, miR-1290, miR-210, miR-29b, miR-32, integrin alpha5, cell adhesion, cell proliferation, cell invasion
- MeSH terms
- Cell Adhesion, Cell Transformation, Neoplastic, Collagen, Drug Combinations, Extracellular Matrix, Humans, Laminin, Neoplasm Invasiveness, Proteoglycans, Gene Expression Regulation, Neoplastic, Cell Culture Techniques, Oligonucleotide Array Sequence Analysis, MicroRNAs, Cell Line, Tumor, Cell Proliferation
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