Cited by 125Hydrogels: synthesis, properties, applications3D Printing in Biomedical ResearchCellular Mechanics and Interactions

Polysaccharide matrices used in 3D in vitro cell culture systems

Diekjürgen, Dorina, Grainger, David W.·2017·Biomaterials

Abstract

Polysaccharides comprise a diverse class of polymeric materials with a history of proven biocompatibility and continual use as biomaterials. Recent focus on new matrices appropriate for three-dimensional (3D) cell culture offers new opportunities to apply polysaccharides as extracellular matrix mimics. However, chemical and structural bases for specific cell-polysaccharide interactions essential for their utility as 3-D cell matrices are not well defined. This review describes how these naturally sourced biomaterials satisfy several key properties for current 3D cell culture needs and can also be synthetically modified or blended with additional components to tailor their cell engagement properties. Beyond their benign interactions with many cell types in cultures, their economical and high quality sourcing, optical clarity for ex situ analytical interrogation and in situ gelation represent important properties of these polymers for 3D cell culture applications. Continued diversification of their versatile glycan chemistry, new bio-synthetic sourcing strategies and elucidation of new cell-specific properties are attractive to expand the polysaccharide polymer utility for cell culture needs. Many 3D cell culture priorities are addressed with the portfolio of polysaccharide materials available and under development. This review provides a critical analysis of their properties, capabilities and challenges in 3D cell culture applications.

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Crossref · 10.1016/j.biomaterials.2017.06.020

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324 of 324 references verified0 citation errors · 0 not checkable

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  • The polyanionic behaviour of xanthan gum due to its acetic and pyruvic acid groups influences gluten-free batter properties, allowing more interactions with water through hydrogen bonds.

    “According to Diekjürgen & Grainger (Diekjürgen & Grainger, 2017), this phenomenon is an effect of the polyanionic behaviour of xanthan gum due to the acetic and pyruvic acid groups of its chemical structure.These acid groups influence the GF batter properties, allowing more interactions with water through hydrogen bonds.”
  • Xanthan gum is largely used in gluten-free breadmaking because it can hydrate in cold water and produce a viscous solution with strong shear-thinning flow behaviour.

    “Xanthan gum, a polysaccharide produced by the bacterium Xanthomonas campestris, is largely used in GF breadmaking, because it can hydrate in cold water and produce a viscous solution with strong shear-thinning flow behaviour (Diekjürgen & Grainger, 2017;Peressini, Pin, & Sensidoni, 2011).”

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Authors
Diekjürgen, D., & Grainger, D. W.
Affiliations
University of Utah (US)
Corresponding author
Grainger, David W.
Venue
Biomaterials
Publisher
Elsevier BV
Published
2017-10
Volume
141
Pages
96-115
ISSN
0142-9612 (ISSN-L)
OpenAlex
W2701735813
Cited by
125
References
329
Citations by year
2026: 7 · 2025: 10 · 2024: 18 · 2023: 14 · 2022: 21 · 2021: 16 · 2020: 8 · 2019: 19 · 2018: 11 · 2017: 1
FWCI
0.69
Type
journal article
Language
en
Open access
closed
Locations
  • Biomaterials (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
polysaccharides, 3D cell culture, extracellular matrix mimics, biocompatibility, in situ gelation
MeSH terms
Animals, Biocompatible Materials, Extracellular Matrix, Glycosaminoglycans, Humans, Polysaccharides, Cell Culture Techniques, Hydrogels, Tissue Scaffolds

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