Cited by 137Tea Polyphenols and EffectsBarrier Structure and Function StudiesAlzheimer's disease research and treatments

Blood Brain Barrier Permeability of (-)-epigallocatechin Gallate, its Proliferation-Enhancing Activity of Human Neuroblastoma SH-Sy5y Cells, and its Preventive Effect on Age-Related Cognitive Dysfunction in Mice

Open access
Pervin, Monira, Unno, Keiko, Nakagawa, Aimi, Takahashi, Yuu, Iguchi, Kazuaki, Yamamoto, Hiroyuki, Hoshino, Minoru, Hara, Aya +5 more·2017·Biochemistry and Biophysics Reports

Abstract

The consumption of green tea catechins (GTCs) suppresses age-related cognitive dysfunction in mice. GTCs are composed of several catechins, of which epigallocatechin gallate (EGCG) is the most abundant, followed by epigallocatechin (EGC). Orally ingested EGCG is hydrolyzed by intestinal biota to EGC and gallic acid (GA). To understand the mechanism of action of GTCs on the brain, their permeability of the blood brain barrier (BBB) as well as their effects on cognitive function in mice and on nerve cell proliferation in vitro were examined. The BBB permeability of EGCG, EGC and GA was examined using a BBB model kit. SAMP10, a mouse model of brain senescence, was used to test cognitive function in vivo. Human neuroblastoma SH-SY5Y cells were used to test nerve cell proliferation and differentiation. The in vitro BBB permeability (%, in 30 min) of EGCG, EGC and GA was 2.8±0.1, 3.4±0.3 and 6.5±0.6, respectively. The permeability of EGCG into the BBB indicates that EGCG reached the brain parenchyma even at a very low concentration. The learning ability of SAMP10 mice that ingested EGCG (20 mg/kg) was significantly higher than of mice that ingested EGC or GA. However, combined ingestion of EGC and GA showed a significant improvement comparable to EGCG. SH-SY5Y cell growth was significantly enhanced by 0.05 µM EGCG, but this effect was reduced at higher concentrations. The effect of EGC and GA was lower than that of EGCG at 0.05 µM. Co-administration of EGC and GA increased neurite length more than EGC or GA alone. Cognitive dysfunction in mice is suppressed after ingesting GTCs when a low concentration of EGCG is incorporated into the brain parenchyma via the BBB. Nerve cell proliferation/differentiation was enhanced by a low concentration of EGCG. Furthermore, the additive effect of EGC and GA suggests that EGCG sustains a preventive effect after the hydrolysis to EGC and GA.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.bbrep.2016.12.012

Cites

38 of 38 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Pervin, M., Unno, K., Nakagawa, A., Takahashi, Y., Iguchi, K., Yamamoto, H., Hoshino, M., Hara, A., Takagaki, A., Nanjo, F., Minami, A., Imai, S., & Nakamura, Y.
Affiliations
  • Shizuoka University (JP)
  • Mitsui (Japan) (JP)
  • Tokyo University of Technology (JP)
Corresponding author
Unno, Keiko
Venue
Biochemistry and Biophysics Reports
Publisher
Elsevier BV
Published
2017-03
Volume
9
Pages
180-186
ISSN
2405-5808 (ISSN-L)
OpenAlex
W2569912524
Cited by
137
References
38
Citations by year
2026: 8 · 2025: 15 · 2024: 7 · 2023: 18 · 2022: 19 · 2021: 22 · 2020: 23 · 2019: 14 · 2018: 10 · 2017: 1
FWCI
10.31
Type
journal article
Language
en
Open access
Locations
  • Biochemistry and Biophysics Reports (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
KAKENHI (23617014, 15K00828)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Tea Polyphenols and Effects
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
epigallocatechin gallate, green tea catechins, blood-brain barrier permeability, epigallocatechin, gallic acid, SH-SY5Y cells, neuronal proliferation, neurite length, learning ability, brain aging, cognitive enhancement

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review