Expressing and functionally evaluating anticancer bispecific antibody fragments using cyanobacterium Synechocystis sp. PCC6803 for sustainable production
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Kasai et al. demonstrated production of functional anticancer antibody fragments in Synechocystis sp. PCC 6803, including scFv, VHH and bispecific tandem VHH formats, with the bispecific VHH retaining target-binding and cytotoxic activity.
“More recently, Kasai et al. demonstrated the production of functional anticancer antibody fragments in Synechocystis sp. PCC 6803, including scFv, VHH and bispecific tandem VHH formats, with the bispecific VHH retaining target-binding and cytotoxic activity [191].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [191]
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He, H., Gao, K., Mao, B., Shi, G., Li, Y., & Deng, X. (2025) · Biotechnology Journal · doi:10.1002/biot.70162 · Cited by 4 · see citing works
Walshe, J., & Saha, S. K. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48060638 · Cited by 1 · see citing works
“More recently, Kasai et al. demonstrated the production of functional anticancer antibody fragments in Synechocystis sp. PCC 6803, including scFv, VHH and bispecific tandem VHH formats, with the bispecific VHH retaining target-binding and cytotoxic activity [191].”
Moreno-Cabezuelo, J. Á., Booth, A., Lin, D., Gathani, K., Kim, D. S., & Sagaram, U. S. (2026) · Biomolecules · doi:10.3390/biom16060870
Cyanobacteria: A sustainable platform for high-value biopharmaceutical protein production
Majhi, B. K. (2026) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2026.101807
Yang, X., Ren, J., Li, Z., Liang, R., & Liu, L. (2026) · Nano Today · doi:10.1016/j.nantod.2026.103206
- Authors
- Kasai, G., Shioya, S., Kobe, M., Kobayashi, S., Takamatsu, S., Makabe, K., Kumagai, I., Sode, K., & Asano, R.
- Affiliations
- Tokyo University of Agriculture and Technology (JP)
- Yamagata University (JP)
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Asano, Ryutaro
- Venue
- Biochemical Engineering Journal
- Publisher
- Elsevier BV
- Published
- 2025-09
- Volume
- 221
- Pages
- 109776
- ISSN
- 1369-703X (ISSN-L)
- OpenAlex
- W4410266180
- Cited by
- 5
- References
- 55
- Citations by year
- 2026: 4 · 2025: 1
- FWCI
- 2.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biochemical Engineering Journal (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- Synechocystis sp. PCC 6803, scFv, VHH, sustainable biomanufacturing, antibody binding activity, cytotoxicity
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