Cited by 5Monoclonal and Polyclonal Antibodies ResearchTransgenic Plants and ApplicationsProtein purification and stability

Expressing and functionally evaluating anticancer bispecific antibody fragments using cyanobacterium Synechocystis sp. PCC6803 for sustainable production

Kasai, Gota, Shioya, Sayumi, Kobe, Miku, Kobayashi, Shunichi, Takamatsu, Shouhei, Makabe, Koki, Kumagai, Izumi, Sode, Koji +1 more·2025·Biochemical Engineering Journal

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Registry

Crossref · 10.1016/j.bej.2025.109776

Cites

54 of 54 references verified0 citation errors · 0 not checkable

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  • Kasai et al. demonstrated production of functional anticancer antibody fragments in Synechocystis sp. PCC 6803, including scFv, VHH and bispecific tandem VHH formats, with the bispecific VHH retaining target-binding and cytotoxic activity.

    “More recently, Kasai et al. demonstrated the production of functional anticancer antibody fragments in Synechocystis sp. PCC 6803, including scFv, VHH and bispecific tandem VHH formats, with the bispecific VHH retaining target-binding and cytotoxic activity [191].”

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Authors
Kasai, G., Shioya, S., Kobe, M., Kobayashi, S., Takamatsu, S., Makabe, K., Kumagai, I., Sode, K., & Asano, R.
Affiliations
  • Tokyo University of Agriculture and Technology (JP)
  • Yamagata University (JP)
  • University of North Carolina at Chapel Hill (US)
Corresponding author
Asano, Ryutaro
Venue
Biochemical Engineering Journal
Publisher
Elsevier BV
Published
2025-09
Volume
221
Pages
109776
ISSN
1369-703X (ISSN-L)
OpenAlex
W4410266180
Cited by
5
References
55
Citations by year
2026: 4 · 2025: 1
FWCI
2.90
Type
journal article
Language
en
Open access
closed
Locations
Biochemical Engineering Journal (publishedVersion)
Funders
Japan Society for the Promotion of Science
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
Keywords
Synechocystis sp. PCC 6803, scFv, VHH, sustainable biomanufacturing, antibody binding activity, cytotoxicity

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

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