Matrigel improves functional properties of human submandibular salivary gland cell line
Abstract
Sjogren's syndrome and radiotherapy for head and neck cancers result in irreversible damage to functional salivary tissue, for which no adequate treatment is available. The microenvironment for salivary gland cell cytodifferentiation is critical for the future development of salivary gland regeneration, repair and tissue engineering treatments. Results from this study indicate that human submandibular cell line (HSG) cultured on Matrigel (2mg/ml) could be induced to differentiate into polarized secretory acinar-like cells. The HSG cells grown on Matrigel were evaluated by physiological functional assays, molecular and immunohistochemistry, immunofluorescence, and morphological assessments. The results showed (1) a decrease in cell proliferation; (2) an increase in cell apoptosis; (3) cellular polarization evident by transepithelial electrical resistance (TER), expressions of tight junction proteins (claudin-1, -2, -3, -4, occludin, JAM-A, and ZO-1) and transmission electron microscopy (TEM); (4) an increase in the production and/or secretion of acinar cell proteins, i.e., alpha-amylase, aquaporin-5, cytokeratins, and mucin-1, that were not associated with increases in mRNA transcription; (5) a decrease in vimentin expression; and (6) expression of potential stem cell biomarkers CD44 and CD166. The data indicated that Matrigel provided a suitable microenvironment for morphological and functional differentiation of HSG cells into 3D acinar like cells. This study provides an in vitro model and baseline data on future developments of new strategies for salivary gland regeneration and replacement.
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A variety of cell lines showed reduced proliferation rate in 3D cultures compared to those cultured in 2D.
“A variety of cell lines showed reduced proliferation rate in 3D cultures compared to those cultured in 2D. 17,27-31”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [31]
Proliferation rates of cells cultured in 3D and 2D are usually different and are cell line and matrix dependent.
“The proliferation rates of cells cultured in 3D and 2D are usually different, and are cell line and matrix dependent.”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [31]
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- Authors
- Maria, O. M., Maria, O., Liu, Y., Komarova, S. V., & Tran, S. D.
- Affiliations
- Mansoura University (EG)
- McGill University (CA)
- Corresponding author
- Tran, Simon D.
- Venue
- The International Journal of Biochemistry & Cell Biology
- Publisher
- Elsevier BV
- Published
- 2011-04
- Volume
- 43
- Issue
- 4
- Pages
- 622-631
- ISSN
- 1357-2725 (ISSN-L)
- OpenAlex
- W2054839459
- Cited by
- 82
- References
- 50
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 6 · 2023: 6 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 4 · 2018: 9 · 2017: 7 · 2016: 8 · 2015: 7 · 2014: 4 · 2013: 8 · 2012: 2
- FWCI
- 3.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The International Journal of Biochemistry & Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Salivary Gland Disorders and Functions
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Keywords
- Matrigel, salivary gland regeneration, acinar differentiation, Sjögren's syndrome, head and neck radiotherapy, tight junction proteins, transepithelial electrical resistance, cell polarization, apoptosis, cell proliferation, stem cell markers, CD44, CD166, vimentin, mucin 1, aquaporin 5, alpha-amylase, salivary gland tissue engineering
- MeSH terms
- Cell Line, Collagen, Drug Combinations, Gene Expression Regulation, Humans, Laminin, Proteoglycans, Submandibular Gland, Protein Biosynthesis, Biomarkers, Tissue Engineering, Cell Proliferation
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