Cited by 82Salivary Gland Disorders and FunctionsTissue Engineering and Regenerative MedicinePancreatic function and diabetes

Matrigel improves functional properties of human submandibular salivary gland cell line

Maria, Ola M., Maria, Osama, Liu, Younan, Komarova, Svetlana V., Tran, Simon D.·2011·The International Journal of Biochemistry & Cell Biology

Abstract

Sjogren's syndrome and radiotherapy for head and neck cancers result in irreversible damage to functional salivary tissue, for which no adequate treatment is available. The microenvironment for salivary gland cell cytodifferentiation is critical for the future development of salivary gland regeneration, repair and tissue engineering treatments. Results from this study indicate that human submandibular cell line (HSG) cultured on Matrigel (2mg/ml) could be induced to differentiate into polarized secretory acinar-like cells. The HSG cells grown on Matrigel were evaluated by physiological functional assays, molecular and immunohistochemistry, immunofluorescence, and morphological assessments. The results showed (1) a decrease in cell proliferation; (2) an increase in cell apoptosis; (3) cellular polarization evident by transepithelial electrical resistance (TER), expressions of tight junction proteins (claudin-1, -2, -3, -4, occludin, JAM-A, and ZO-1) and transmission electron microscopy (TEM); (4) an increase in the production and/or secretion of acinar cell proteins, i.e., alpha-amylase, aquaporin-5, cytokeratins, and mucin-1, that were not associated with increases in mRNA transcription; (5) a decrease in vimentin expression; and (6) expression of potential stem cell biomarkers CD44 and CD166. The data indicated that Matrigel provided a suitable microenvironment for morphological and functional differentiation of HSG cells into 3D acinar like cells. This study provides an in vitro model and baseline data on future developments of new strategies for salivary gland regeneration and replacement.

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Crossref · 10.1016/j.biocel.2011.01.001

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Authors
Maria, O. M., Maria, O., Liu, Y., Komarova, S. V., & Tran, S. D.
Affiliations
  • Mansoura University (EG)
  • McGill University (CA)
Corresponding author
Tran, Simon D.
Venue
The International Journal of Biochemistry & Cell Biology
Publisher
Elsevier BV
Published
2011-04
Volume
43
Issue
4
Pages
622-631
ISSN
1357-2725 (ISSN-L)
OpenAlex
W2054839459
Cited by
82
References
50
Citations by year
2026: 4 · 2025: 3 · 2024: 6 · 2023: 6 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 4 · 2018: 9 · 2017: 7 · 2016: 8 · 2015: 7 · 2014: 4 · 2013: 8 · 2012: 2
FWCI
3.06
Type
journal article
Language
en
Open access
closed
Locations
  • The International Journal of Biochemistry & Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Salivary Gland Disorders and Functions
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
Keywords
Matrigel, salivary gland regeneration, acinar differentiation, Sjögren's syndrome, head and neck radiotherapy, tight junction proteins, transepithelial electrical resistance, cell polarization, apoptosis, cell proliferation, stem cell markers, CD44, CD166, vimentin, mucin 1, aquaporin 5, alpha-amylase, salivary gland tissue engineering
MeSH terms
Cell Line, Collagen, Drug Combinations, Gene Expression Regulation, Humans, Laminin, Proteoglycans, Submandibular Gland, Protein Biosynthesis, Biomarkers, Tissue Engineering, Cell Proliferation

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