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Cited by 109

Implication of apoE isoforms in cholesterol metabolism by primary rat hippocampal neurons and astrocytes.

Rapp, Alfred, Gmeiner, Bernhard, Hüttinger, Manfred·2006·Biochimie
DOI 10.1016/j.biochi.2005.10.007
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Abstract

Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content of hippocampal plasma membrane coincides with the disease. In this study, we show for the first time that the apoE dependent cholesterol metabolism in hippocampal neurons is higher than that of hippocampal astrocytes. Further, apoE-bound cholesterol is highly incorporated in membranous compartments in hippocampal neurons, whereas hippocampal astrocytes show higher intracellular distribution. This is an effect that coincides with cell-type dependent difference of low density lipoprotein receptor (LDLR) family member expression. Hippocampal neurons express high levels of the LDLR related protein (LRP), whereas hippocampal astrocytes are highly positive for LDLR. We could also demonstrate an apoE isoform (apoE2, apoE3 and apoE4) dependent cholesterol uptake in both cells types. In hippocampal neurons, we could find a decreased apoE4-bound cholesterol uptake. In contrast, hippocampal astrocytes show decreased internalization of apoE2-bound cholesterol. In addition, lipidated apoE4 is little associated with neurites in hippocampal neurons in comparison to the other two isoforms. In contrary, hippocampal astrocytes show faint apoE2 immunocytostaining intensity. Data p…

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Authors
Rapp, A., Gmeiner, B., & Hüttinger, M.
Venue
Biochimie
Publisher
Elsevier BV
Published
2006-05
Volume
88
Issue
5
Pages
473-483
ISSN
0300-9084
DOI
10.1016/j.biochi.2005.10.007
Cited by
109
References
5
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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