Complete structural characterization of ceramides as [M−H]− ions by multiple-stage linear ion trap mass spectrometry
Open accessAbstract
Ceramide is a huge lipid family consisting of diversified structures including various modifications in the fatty acyl chain and the long chain base (LCB). In this contribution, negative-ion ESI linear ion-trap multiple-stage mass spectrometric method (LIT MS n) towards complete structural determination of ceramides in ten major families characterized as the [M-H]- ions is described. Multiple sets of fragment ions reflecting the fatty acyl chain and LCB were observed in the CID MS 2 spectrum, while the sequential MS 3 and MS 4 spectra contain structural information for locating the double bond and the functional groups, permitting realization of the fragmentation processes. Thereby, differentiation of ceramide molecules varied by chain length, the LCB (sphingosine, phytosphigosine, 6-hydroxy-sphingosine), and by the modification (α-hydroxy-, β-hydroxy-, ω-hydroxy-FA) can be achieved; and many isomeric structures in the biological specimen can be revealed in detail.
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The fragmentation pattern of the peak at 12.86 min is consistent with that of C26:0 BS in previous reports.
“The fragmentation pattern of this peak is consistent with that of C26:0 BS in previous reports (12,42), demonstrating that the peak detected at 12.86 min was C26:0 BS.”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [42]
The peak detected at 12.86 min in mouse epidermis was C26:0 BS.
“The fragmentation pattern of this peak is consistent with that of C26:0 BS in previous reports (12,42), demonstrating that the peak detected at 12.86 min was C26:0 BS.”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [42]
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- Authors
- Hsu, F.-F.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Hsu, Fong-Fu
- ORCID
- Hsu, Fong-Fu
- Venue
- Biochimie
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 130
- Pages
- 63-75
- ISSN
- 0037-9042 (ISSN-L)
- 0300-9084
- PMC
- PMC5086283
- OpenAlex
- W2510701441
- Cited by
- 48
- References
- 33
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 10 · 2022: 5 · 2021: 5 · 2020: 8 · 2019: 9 · 2018: 3 · 2017: 2 · 2016: 1
- FWCI
- 2.49
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biochimie (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- US Public Health Service (P41-GM103422, P60-DK-20579, P30-DK56341, 4R33HL120760-03)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- ceramide, linear ion trap mass spectrometry, structural characterization, fatty acyl chains, long-chain bases, sphingosine, phytosphingosine, hydroxy fatty acids
- MeSH terms
- Ceramides, Fatty Acids, Ions, Isomerism, Models, Chemical, Sphingosine, Molecular Structure, Spectrometry, Mass, Electrospray Ionization
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