Cited by 322Sphingolipid Metabolism and SignalingMetabolomics and Mass Spectrometry StudiesAdvancements in Transdermal Drug Delivery

Characterization of overall ceramide species in human stratum corneum

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Masukawa, Yoshinori, Narita, Hirofumi, Shimizu, Eri, Kondo, Naoki, Sugai, Yoshiya, Oba, Tsuyoshi, Homma, Rika, Ishikawa, Junko +4 more·2008·Journal of Lipid Research

Abstract

Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse CER species, however, have not been comprehensively revealed. The aim of this study was to characterize overall CER species in the SC. First, we constructed 3D multi-mass chromatograms of the overall CER species, based on normal-phase liquid chromatography (NPLC) connected to electrospray ionization-mass spectrometry (ESI-MS) using a gradient elution system and a postcolumn addition of a volatile salt-containing polar solvent. The CERs targeted from the 3D chromatograms were structurally analyzed using NPLC-ESI-tandem mass spectrometry (MS/MS), which resulted in the identification of 342 CER species in the inner forearm SC. This led to the discovery of a new CER class consisting of alpha-hydroxy fatty acid and dihydrosphingosine moieties, in addition to the 10 classes generally known. The results also revealed that those CERs contain long-chain (more than C(18))-containing sphingoids and a great number of isobaric species. These novel results will contribute not only to physiochemical research on CERs in the SC but also to lipidomics approaches to CERs in the skin.

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Authors
Masukawa, Y., Narita, H., Shimizu, E., Kondo, N., Sugai, Y., Oba, T., Homma, R., Ishikawa, J., Takagi, Y., Kitahara, T., Takema, Y., & Kita, K.
Affiliations
Kao Corporation (Japan) (JP)
Corresponding author
Masukawa, Yoshinori
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
2008-07
Volume
49
Issue
7
Pages
1466-1476
ISSN
0022-2275 (ISSN-L)
OpenAlex
W2159423422
Cited by
322
References
52
Citations by year
2026: 7 · 2025: 6 · 2024: 17 · 2023: 13 · 2022: 17 · 2021: 19 · 2020: 22 · 2019: 18 · 2018: 24 · 2017: 25 · 2016: 24 · 2015: 19 · 2014: 25 · 2013: 30 · 2012: 19
FWCI
4.94
Type
journal article
Language
en
Open access
Locations
  • Journal of Lipid Research (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Kao Corporation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
Keywords
ceramide, stratum corneum, skin barrier, dihydrosphingosine, keratinization, normal-phase chromatography, tandem mass spectrometry
MeSH terms
Ceramides, Humans, Skin, Mass Spectrometry, Molecular Structure

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