Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro
Abstract
An electrospinning method was used to fabricate silk fibroin (SF) nanofiber nonwovens for cell culture of normal human keratinocytes and fibroblasts. The electrospinning of regenerated SF was performed with formic acid as a spinning solvent. For insolubilization, as-spun SF nanofiber nonwovens were chemically treated with an aqueous methanol solution of 50%. Morphology and microstructure of as-spun and chemically treated SF nanofibers were investigated by scanning electron microscopy and mercury porosimetry. As-spun SF nanofibers exhibited a circular cross-section with a smooth surface. From the image analysis, they had an average diameter of 80 nm and their diameters ranged from 30 to 120 nm. During the chemical treatment for 60 min, porosity of nonwovens composed of SF nanofibers decreased from 76.1% up to 68.1%. To assay the cytocompatibility and cell behavior onto the electrospun SF nanofibers, cell attachment and spreading of normal human keratinocytes and fibroblasts seeded on the SF nanofibers and interaction between cells and SF nanofibers were studied. Cell morphology on SF nanofibers was examined by scanning electron microscopy. Our results indicate that the SF nanofibers may be a good candidate for the biomedical applications, such as wound dressing and scaffolds for tissue engineering.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The diameter of silk fibroin nanofibers produced by electrospinning closely resembles that of fibronectin in the extracellular matrix; some researchers achieved fibers with an average diameter of 80 nm using formic acid as a solvent.
“The diameter of nanofibers produced through this method closely resembles that of fibronectin in the extracellular matrix (for instance, some researchers have achieved fibers with an average diameter of 80 nm using formic acid as a solvent [74]), thereby more accurately mimicking the structure of the extracellular matrix.”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [74]
Cites
Geometric Control of Cell Life and Death
Chen, C. S., Mrksich, M., Huang, S., Whitesides, G. M., & Ingber, D. E. (1997) · Science · doi:10.1126/science.276.5317.1425 · Cited by 4,628 · see citing works
Electrospun nanofibrous structure: A novel scaffold for tissue engineering
Li, W., Laurencin, C. T., Caterson, E. J., Tuan, R. S., & Ko, F. (2002) · Journal of Biomedical Materials Research · doi:10.1002/jbm.10167 · Cited by 2,429 · see citing works
Transport properties of porous membranes based on electrospun nanofibers
Gibson, P., Schreuder‐Gibson, H. L., & Rivin, D. (2001) · Colloids and Surfaces A Physicochemical and Engineering Aspects · doi:10.1016/s0927-7757(01)00616-1 · Cited by 879 · see citing works
Electrospinning for tissue engineering scaffolds
Lannutti, J., Reneker, D., Ma, T., Tomasko, D., & Farson, D. (2007) · Materials Science and Engineering: C · doi:10.1016/j.msec.2006.05.019 · Cited by 693 · see citing works
Attachment and growth of cultured fibroblast cells on silk protein matrices
Minoura, N., Aiba, S., Gotoh, Y., Tsukada, M., & Imai, Y. (1995) · Journal of Biomedical Materials Research · doi:10.1002/jbm.820291008 · Cited by 373 · see citing works
Kaplan, D. L., Adams, W. W., Farmer, B. L., et al. (1993) · ACS symposium series · doi:10.1021/bk-1994-0544 · Cited by 233 · see citing works
Spatially controlled cell engineering on biodegradable polymer surfaces
Patel, N., Padera, R. F., Sanders, G. H. W., Cannizzaro, S. M., Davies, M. C., Langer, R., Roberts, C. J., Tendler, S. J. B., Williams, P. M., & Shakesheff, K. M. (1998) · The FASEB Journal · doi:10.1096/fasebj.12.14.1447 · Cited by 233 · see citing works
Cai, K., Yao, K., Lin, S., Yang, Z., Li, X., Xie, H., Qing, T., & Gao, L. (2002) · Biomaterials · doi:10.1016/s0142-9612(01)00230-7 · Cited by 184 · see citing works
Cai, K., Yao, K., Cui, Y., et al. (2002) · Biomaterials · doi:10.1016/s0142-9612(01)00287-3 · Cited by 158 · see citing works
The network structure of corneal fibroblasts in the rat as revealed by scanning electron microscopy.
Nishida, T., Yasumoto, K., Otori, T., et al. (1988) · PubMed · Cited by 143 · see citing works
van Kooten, T. G., Whitesides, J. F., & von Recum, A. F. (1998) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(199821)43:1 · Cited by 142 · see citing works
In vivo blood compatibility of regenerated silk fibroin.
Sakabe, H., Ito, H., Miyamoto, T., Noishiki, Y., & Ha, W. S. (1989) · Sen'i Gakkaishi · doi:10.2115/fiber.45.11_487 · Cited by 124 · see citing works
Molecular Basis of Ligand Recognition by Integrin α5β1
Mould, A. P., Askari, J. A., & Humphries, M. J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m000572200 · Cited by 95 · see citing works
Gotoh, Y. (1997) · Biomaterials · doi:10.1016/s0142-9612(96)00137-8 · Cited by 86 · see citing works
Molecular Basis of Ligand Recognition by Integrin α5β1
Humphries, J. D., Askari, J. A., Zhang, X.-P., Takada, Y., Humphries, M. J., & Mould, A. P. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m000568200 · Cited by 74 · see citing works
Relationships between antithrombogenicity and surface free energy of regenerated silk fibroin films
Park, W. H., Ha, W. S., Ito, H., Miyamoto, T., Inagaki, H., & D, Y. N. M. (2001) · Fibers and Polymers · doi:10.1007/bf02875259 · Cited by 46 · see citing works
Self-citationImpedance spectroscopy of protein polymer modified silicon micromachined probes
Athreya, S. A., & Martin, D. C. (1999) · Sensors and Actuators A Physical · doi:10.1016/s0924-4247(98)00223-4 · Cited by 28 · see citing works
Vasin, S. L., Rosanova, I. B., & Sevastianov, V. I. (1998) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(19980305)39:3 · Cited by 26 · see citing works
MALIGNANT TRANSFORMATION OF HPV-IMMORTALIZED HUMAN ORAL KERATINOCYTES BY CHEMICAL CARCINOGENS
Min, B.-M., WOO, K., BAEK, J., et al. (1995) · International Journal of Oncology · doi:10.3892/ijo.7.2.249 · Cited by 11 · see citing works
Self-citationSynthesis of porous macrospheres from amino-polymers
Baltieri, R. C., Mei, L. H. I., Tamashiro, W. M. D. S. C., et al. (2002) · European Polymer Journal · doi:10.1016/s0014-3057(01)00177-x · Cited by 9 · see citing works
Santin, M., Motta, A., Freddi, G., & Cannas, M. F. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(19990905)46:3 · Cited by 4 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Fully Bioabsorbable Natural‐Materials‐Based Triboelectric Nanogenerators
Jiang, W., Li, H., Liu, Z., Li, Z., Tian, J., Shi, B., Zou, Y., Ouyang, H., Zhao, C., Zhao, L., et al. (2018) · Advanced Materials · doi:10.1002/adma.201801895 · Cited by 449 · see citing works
Electrospun polymeric nanofibres as wound dressings: A review
Miguel, S. P., Figueira, D. R., Simões, D., Ribeiro, M. P., Coutinho, P., Ferreira, P., & Correia, I. J. (2018) · Colloids and Surfaces B: Biointerfaces · doi:10.1016/j.colsurfb.2018.05.011 · Cited by 362 · see citing works
Mauck, R. L., Baker, B. M., Nerurkar, N. L., Burdick, J. A., Li, W.-J., Tuan, R. S., & Elliott, D. M. (2009) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2008.0652 · Cited by 213 · see citing works
Research Progress of Shape Memory Polymer and 4D Printing in Biomedical Application
Zhao, W., Yue, C., Liu, L., Liu, Y., & Leng, J. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202201975 · Cited by 183 · see citing works
Park, S. Y., Ki, C. S., Park, Y. H., Jung, H. M., Woo, K. M., & Kim, H. J. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0328 · Cited by 120 · see citing works
Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review
Zhu, S., Zhang, Q., Xu, X., Liu, Z., Cheng, G., Long, D., Cheng, L., & Dai, F. (2025) · Polymers · doi:10.3390/polym17060772 · Cited by 36 · see citing works
“…produced through this method closely resembles that of fibronectin in the extracellular matrix (for instance, some researchers have achieved fibers with an average diameter of 80 nm using formic acid as a solvent [74]), thereby more accurately mimicking the structure of the extracellular matrix.”
- Authors
- Min, B.-M., Lee, G., Kim, S. H., Nam, Y. S., Lee, T. S., & Park, W. H.
- Affiliations
- Seoul National University (KR)
- Chungnam National University (KR)
- Corresponding author
- Park, Won Ho
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2004-03
- Volume
- 25
- Issue
- 7-8
- Pages
- 1289-1297
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2026569399
- Cited by
- 1,151
- References
- 21
- Citations by year
- 2026: 20 · 2025: 25 · 2024: 35 · 2023: 25 · 2022: 48 · 2021: 53 · 2020: 55 · 2019: 63 · 2018: 63 · 2017: 60 · 2016: 71 · 2015: 51 · 2014: 89 · 2013: 55 · 2012: 79
- FWCI
- 16.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Funders
- Seoul National University
- Topics
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- silk fibroin, electrospinning, nanofibers, keratinocytes, fibroblasts, cell adhesion, cell spreading, cytocompatibility, nonwoven fabrics, porosity, chemical treatment, wound dressings, tissue engineering scaffolds
- MeSH terms
- Adolescent, Adult, Biocompatible Materials, Cell Adhesion, Cell Division, Cell Movement, Cells, Cultured, Fibroblasts, Fibroins, Humans, Manufactured Materials, Rotation, Textiles, Keratinocytes, Porosity, Insect Proteins, Tissue Engineering, Nanotechnology, Silk, Static Electricity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
