Electrospun Silk Fibroin Scaffolds with Macropores for Bone Regeneration: An In Vitro and In Vivo Study
Abstract
We developed three-dimensional electrospun silk fibroin (ESF) scaffolds with controllable pore size. The purpose of this study was to evaluate ESF scaffolds with pores (P-ESF) for bone regeneration via in vitro and in vivo studies, with a comparison to a commercially available porous three-dimensional polylactic acid (PLA) scaffold. P-ESF supported significantly higher proliferation and alkaline phosphatase activity of osteoblasts than PLA in vitro (p < 0.05). Moreover, higher expression levels of activated adhesion-related proteins, including focal adhesion kinase, were observed in the P-ESF than in PLA, as confirmed by western blot analyses. Microcomputed tomography revealed that 78.30% of the original bone volume was attained in the P-ESF implantation group at 7 weeks after critical bone defect formation in rat calvaria. Comparatively, the PLA implantation group showed only 49.31%. Histological evaluation also showed new bone tissue formation upon P-ESF implantation. Taken together, the P-ESF scaffold may be a good bone substitute for bone regeneration.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Silk fibroin scaffolds prepared by electrostatic spinning significantly promote ALP activity and osteogenic differentiation ability of mouse embryonic osteoblast precursor cells (MC3T3-E1).
“The researchers used the three-dimensional porous scaffolds of silk fibroin prepared by electrostatic spinning and three-dimensional PLA scaffolds to carry out experimental comparative studies, and the results showed that the silk fibroin scaffolds could significantly promote the ALP activity and osteogenic differentiation ability of mouse embryonic osteoblast precursor cells (MC3T3-E1);”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [75]
Silk fibroin scaffolds are significantly superior to PLA scaffolds in the ability to form new bone in animal experiments.
“moreover, the silk fibroin scaffolds were significantly superior to the PLA scaffolds in terms of the ability to form new bone in the animal experiments [75], which suggests that electrostatically spun silk fibroin scaffolds have good potential for bone tissue repair.”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [75]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A review on polymer nanofibers by electrospinning and their applications in nanocomposites
Huang, Z., Zhang, Y., Kotaki, M., & Ramakrishna, S. (2003) · Composites Science and Technology · doi:10.1016/s0266-3538(03)00178-7 · Cited by 7,567 · see citing works
Porosity of 3D biomaterial scaffolds and osteogenesis
Karageorgiou, V., & Kaplan, D. L. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.02.002 · Cited by 6,639 · see citing works
Altman, G. H., Diaz, F., Jakuba, C., Calabro, T., Horan, R. L., Chen, J., Lu, H., Richmond, J., & Kaplan, D. L. (2003) · Biomaterials · doi:10.1016/s0142-9612(02)00353-8 · Cited by 3,458 · see citing works
Electrospun nanofibrous structure: A novel scaffold for tissue engineering
Li, W., Laurencin, C. T., Caterson, E. J., Tuan, R. S., & Ko, F. (2002) · Journal of Biomedical Materials Research · doi:10.1002/jbm.10167 · Cited by 2,429 · see citing works
Scaffolding in tissue engineering: general approaches and tissue-specific considerations
Chan, B. P., & Leong, K. W. (2008) · European Spine Journal · doi:10.1007/s00586-008-0745-3 · Cited by 1,708 · see citing works
Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase
Schlaepfer, D. D., Hanks, S. K., Hunter, T., & Geer, P. V. D. (1994) · Nature · doi:10.1038/372786a0 · Cited by 1,595 · see citing works
Athanasiou, K. A., Niederauer, G. G., & Agrawal, C. M. (1996) · Biomaterials · doi:10.1016/0142-9612(96)85754-1 · Cited by 1,497 · see citing works
Min, B.-M., Lee, G., Kim, S. H., Nam, Y. S., Lee, T. S., & Park, W. H. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2003.08.045 · Cited by 1,151 · see citing works
Stem cell-based tissue engineering with silk biomaterials
Wang, Y., Kim, H.-J., Vunjak‐Novakovic, G., & Kaplan, D. L. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.07.008 · Cited by 968 · see citing works
Self-citationThe extracellular matrix as a scaffold for tissue reconstruction
Badylak, S. F. (2002) · Seminars in Cell and Developmental Biology · doi:10.1016/s1084952102000940 · Cited by 819 · see citing works
Biodegradability and biodegradation of poly(lactide)
Tokiwa, Y., & Calabia, B. P. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0488-1 · Cited by 665 · see citing works
Human bone marrow stromal cell responses on electrospun silk fibroin mats
Jin, H., Chen, J., Karageorgiou, V., Altman, G. H., & Kaplan, D. L. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00609-4 · Cited by 631 · see citing works
Integrin signaling to the actin cytoskeleton
DeMali, K. A., Wennerberg, K., & Burridge, K. (2003) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(03)00109-1 · Cited by 504 · see citing works
Mauney, J. R., Nguyen, T., Gillen, K., Kirker-Head, C., Gimble, J. M., & Kaplan, D. L. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.08.017 · Cited by 373 · see citing works
Attachment and growth of fibroblast cells on silk fibroin
Minoura, N., Aiba, S. I., Higuchi, M., Gotoh, Y., Tsukada, M., & Imai, Y. (1995) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1995.1368 · Cited by 371 · see citing works
Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro
Yang, Y., Chen, X., Ding, F., Zhang, P., Liu, J., & Gu, X. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2006.12.004 · Cited by 333 · see citing works
Min, B., Jeong, L., Nam, Y. S., Kim, J., Kim, J. Y., & Park, W. H. (2004) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2004.08.004 · Cited by 209 · see citing works
Silk hydrogel for cartilage tissue engineering
Chao, P. G., Yodmuang, S., Wang, X., Sun, L., Kaplan, D. L., & Vunjak‐Novakovic, G. (2010) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.31686 · Cited by 198 · see citing works
Macrophages promote angiogenesis in human breast tumour spheroids in vivo
Bingle, L., Lewis, C. E., Corke, K. P., Reed, M., & Brown, N. J. (2005) · British Journal of Cancer · doi:10.1038/sj.bjc.6602901 · Cited by 190 · see citing works
Tissue Engineering of Cartilage Using a Hybrid Scaffold of Synthetic Polymer and Collagen
Chen, G., Sato, T., Ushida, T., Ochiai, N., & Tateishi, T. (2004) · Tissue Engineering · doi:10.1089/107632704323061681 · Cited by 172 · see citing works
The mineralization of bone tissue: a forgotten dimension in osteoporosis research
Boivin, G., & Meunier, P. J. (2003) · Osteoporosis International · doi:10.1007/s00198-002-1347-2 · Cited by 159 · see citing works
Ki, C. S., Park, S. Y., Kim, H. J., Jung, H., Woo, K. M., Lee, S. E., & Park, Y. H. (2007) · Biotechnology Letters · doi:10.1007/s10529-007-9581-5 · Cited by 148 · see citing works
Self-citationDeclercq, H., Verbeeck, R. M. H., Ridder, L. I. F. J. M. D., Schacht, E. H., & Cornelissen, M. J. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.01.025 · Cited by 141 · see citing works
Electrospun three‐dimensional silk fibroin nanofibrous scaffold
Ki, C. S., Kim, J. W., Hyun, J. H., Lee, K. H., Hattori, M., Rah, D. K., & Park, Y. H. (2007) · Journal of Applied Polymer Science · doi:10.1002/app.26914 · Cited by 134 · see citing works
Self-citationSilk fibroin in ocular tissue reconstruction
Harkin, D. G., George, K. A., Madden, P. W., Schwab, I. R., Hutmacher, D. W., & Chirilă, T. V. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2010.12.041 · Cited by 130 · see citing works
Caswell, A. M., Whyte, M. P., & Russell, R. G. G. (1991) · Critical Reviews in Clinical Laboratory Sciences · doi:10.3109/10408369109106862 · Cited by 103 · see citing works
Biochemical Markers of Bone Formation
Gundberg, C. M. (2000) · Clinics in Laboratory Medicine · doi:10.1016/s0272-2712(18)30049-0 · Cited by 86 · see citing works
The Effect of Sterilization Methods on the Physical Properties of Silk Sericin Scaffolds
Siritientong, T., Srichana, T., & Aramwit, P. (2011) · AAPS PharmSciTech · doi:10.1208/s12249-011-9641-y · Cited by 83 · see citing works
A fluorescence study on the gel-to-sol transition of κ-carrageenan
Pekcan, Ö., & Tarı, Ö. (2004) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2004.05.002 · Cited by 77 · see citing works
Tyrosine phosphorylation of paxillin affects the metastatic potential of human osteosarcoma
Azuma, K., Tanaka, M., Uekita, T., Inoue, S., Yokota, J., Ouchi, Y., & Sakai, R. (2005) · Oncogene · doi:10.1038/sj.onc.1208654 · Cited by 75 · see citing works
Roohani‐Esfahani, S., Lu, Z., Li, J. J., Ellis-Behnke, R. G., Kaplan, D. L., & Zreiqat, H. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.10.009 · Cited by 74 · see citing works
Bone tissue engineering and repair by gene therapy
Volker, M. B. (2007) · Frontiers in bioscience · doi:10.2741/2724 · Cited by 69 · see citing works
Ikeda, R., Fujioka, H., Nagura, I., Kokubu, T., Toyokawa, N., Inui, A., Makino, T., Kaneko, H., Doita, M., & Kurosaka, M. (2008) · International Orthopaedics · doi:10.1007/s00264-008-0532-0 · Cited by 67 · see citing works
Human dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol based silk scaffolds
Zhang, W., Ahluwalia, I. P., Literman, R., Kaplan, D. L., & Yelick, P. C. (2011) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.33062 · Cited by 61 · see citing works
Fabrication and evaluation of poly(epsilon‐caprolactone)/silk fibroin blend nanofibrous scaffold
Lim, J., Ki, C. S., Kim, J. W., Lee, K. G., Kang, S. W., Kweon, H., & Park, Y. H. (2011) · Biopolymers · doi:10.1002/bip.22016 · Cited by 59 · see citing works
Self-citationChristiansen, P. (2001) · PubMed · Cited by 58 · see citing works
Serum and Drainage Fluid Vascular Endothelial Growth Factor Levels in Early Surgical Wounds
Karayiannakis, A. J., Zbar, A. P., Polychronidis, A., & Simopoulos, C. E. (2003) · European Surgical Research · doi:10.1159/000073388 · Cited by 57 · see citing works
Relationship between bone ingrowth, mineral apposition rate, and osteoblast activity
Bloebaum, R. D., Willie, B. M., Mitchell, B., & Hofmann, A. A. (2007) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31087 · Cited by 53 · see citing works
Kang, S., Kim, K., BYUN, Y.-S., Suh, S., JIM, U.-H., KIM, K.-H., Lee, I., & Kim, C. (2006) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1290/0510068.1 · Cited by 25 · see citing works
Seventy years in the service of science 1924–1994
Rygaard, J. (1994) · Apmis · doi:10.1111/j.1699-0463.1994.tb04838.x · Cited by 21 · see citing works
Garofalo, G. S. (2008) · PubMed · Cited by 17 · see citing works
Elevation of serum alkaline phosphatase in clear cell chondrosarcoma of bone.
Ogose, A., Hotta, T., Kawashima, H., Hatano, K., Umezu, H., Inoue, Y., & Endo, N. (2001) · PubMed · Cited by 16 · see citing works
Biodegradable Composite Implants
Schlichting, K., Dahne, M., & Weiler, A. (2006) · Sports Medicine and Arthroscopy Review · doi:10.1097/00132585-200609000-00009 · Cited by 13 · see citing works
Hakimi, O., Vollrath, F. W., & Carr, A. J. (2011) · Elsevier eBooks · doi:10.1016/b978-0-08-088504-9.00435-9 · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Electrospinning for tissue engineering applications
Rahmati, M., Mills, D. K., Urbanska, A. M., Saeb, M. R., Venugopal, J. R., Ramakrishna, S., & Mozafari, M. (2020) · Progress in Materials Science · doi:10.1016/j.pmatsci.2020.100721 · Cited by 816 · see citing works
Silk fibroin as biomaterial for bone tissue engineering
Melke, J., Midha, S., Ghosh, S., Ito, K., & Hofmann, S. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.09.005 · Cited by 790 · see citing works
Silk Fibroin in Tissue Engineering
Kasoju, N., & Bora, U. (2012) · Advanced Healthcare Materials · doi:10.1002/adhm.201200097 · Cited by 384 · see citing works
Natural and Synthetic Polymers for Bone Scaffolds Optimization
Donnaloja, F., Jacchetti, E., Soncini, M., & Raimondi, M. T. (2020) · Polymers · doi:10.3390/polym12040905 · Cited by 324 · see citing works
Potential applications of silk sericin, a natural protein from textile industry by-products
Aramwit, P., Siritientong, T., & Srichana, T. (2011) · Waste Management & Research The Journal for a Sustainable Circular Economy · doi:10.1177/0734242x11404733 · Cited by 319 · see citing works
Silk scaffolds in bone tissue engineering: An overview
Bhattacharjee, P., Kundu, B., Naskar, D., Kim, H.-W., Maiti, T. K., Bhattacharya, D., & Kundu, S. C. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.09.027 · Cited by 318 · see citing works
Self-citationSilk microfiber-reinforced silk hydrogel composites for functional cartilage tissue repair
Yodmuang, S., McNamara, S. L., Nover, A. B., Mandal, B. B., Agarwal, M., Kelly, T. N., Chao, P. G., Hung, C. T., Kaplan, D. L., & Vunjak‐Novakovic, G. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.09.032 · Cited by 263 · see citing works
Potential of Agarose/Silk Fibroin Blended Hydrogel for in Vitro Cartilage Tissue Engineering
Singh, Y. P., Bhardwaj, N., & Mandal, B. B. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b08285 · Cited by 240 · see citing works
Advancements in Electrospinning of Polymeric Nanofibrous Scaffolds for Tissue Engineering
Ingavle, G. C., & Leach, J. K. (2013) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2013.0276 · Cited by 228 · see citing works
Silk hydrogel for cartilage tissue engineering
Chao, P. G., Yodmuang, S., Wang, X., Sun, L., Kaplan, D. L., & Vunjak‐Novakovic, G. (2010) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.31686 · Cited by 198 · see citing works
Electrospun biodegradable nanofibers scaffolds for bone tissue engineering
Khajavi, R., Abbasipour, M., & Bahador, A. (2015) · Journal of Applied Polymer Science · doi:10.1002/app.42883 · Cited by 177 · see citing works
Zhang, Y., Liu, J., Huang, L., Wang, Z., & Wang, L. (2015) · Scientific Reports · doi:10.1038/srep12374 · Cited by 175 · see citing works
Kim, H., Che, L., Ha, Y., & Ryu, W. (2014) · Materials Science and Engineering C · doi:10.1016/j.msec.2014.04.012 · Cited by 171 · see citing works
Self-citationAdvanced Silk Fibroin Biomaterials for Cartilage Regeneration
Cheng, G., Davoudi, Z., Xing, X., Yu, X., Cheng, X., Li, Z., Deng, H., & Wang, Q. (2018) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b00150 · Cited by 162 · see citing works
3D electrospun silk fibroin nanofibers for fabrication of artificial skin
Sheikh, F. A., Ju, H. W., Lee, J. M., Moon, B. M., Park, H. J., Lee, O. J., Kim, J.-H., Kim, D., & Park, C. H. (2014) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2014.11.007 · Cited by 161 · see citing works
Shao, W., He, J., Sang, F., Ding, B., Chen, L., Cui, S., Li, K., Han, Q., & Tan, W. (2015) · Materials Science and Engineering C · doi:10.1016/j.msec.2015.08.046 · Cited by 160 · see citing works
Three‐dimensional electrospun nanofibrous scaffolds for bone tissue engineering
Lin, W., Chen, M., Qu, T., Li, J., & Man, Y. (2019) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.34479 · Cited by 137 · see citing works
Scaffold-based regeneration of skeletal tissues to meet clinical challenges
Li, J. J., Kaplan, D. L., & Zreiqat, H. (2014) · Journal of Materials Chemistry B · doi:10.1039/c4tb01073f · Cited by 128 · see citing works
Silk fibroin in ocular tissue reconstruction
Harkin, D. G., George, K. A., Madden, P. W., Schwab, I. R., Hutmacher, D. W., & Chirilă, T. V. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2010.12.041 · Cited by 130 · see citing works
Silk fibroin-based biomaterials for musculoskeletal tissue engineering
Ma, D., Wang, Y., & Dai, W. (2018) · Materials Science and Engineering C · doi:10.1016/j.msec.2018.04.062 · Cited by 122 · see citing works
Silkworm and spider silk electrospinning: a review
Belbéoch, C., Lejeune, J., Vroman, P., & Salaün, F. (2021) · Environmental Chemistry Letters · doi:10.1007/s10311-020-01147-x · Cited by 121 · see citing works
Tissue Regeneration: A Silk Road
Jao, D., Mou, X., & Hu, X. (2016) · Journal of Functional Biomaterials · doi:10.3390/jfb7030022 · Cited by 114 · see citing works
Lee, S. Y., Jeon, S., Kwon, Y. W., Kwon, M., Kang, M. S., Seong, K., Park, T.-E., Yang, S. Y., Han, D., Hong, S. W., et al. (2022) · Science Advances · doi:10.1126/sciadv.abn1646 · Cited by 108 · see citing works
Ranakoti, L., Gangil, B., Bhandari, P., Singh, T., Sharma, S. K., Singh, J., & Singh, S. (2023) · Molecules · doi:10.3390/molecules28020485 · Cited by 101 · see citing works
Lee, J. M., Chae, T., Sheikh, F. A., Ju, H. W., Moon, B. M., Park, H. J., Park, Y. R., & Park, C. H. (2016) · Materials Science and Engineering C · doi:10.1016/j.msec.2016.06.019 · Cited by 97 · see citing works
Self-citationRegeneration of the anterior cruciate ligament: Current strategies in tissue engineering
Nau, T. (2015) · World Journal of Orthopedics · doi:10.5312/wjo.v6.i1.127 · Cited by 92 · see citing works
The Effect of Sterilization Methods on the Physical Properties of Silk Sericin Scaffolds
Siritientong, T., Srichana, T., & Aramwit, P. (2011) · AAPS PharmSciTech · doi:10.1208/s12249-011-9641-y · Cited by 83 · see citing works
Goonoo, N., Khanbabaee, B., Steuber, M., Bhaw‐Luximon, A., Jonas, U., Pietsch, U., Jhurry, D., & Schönherr, H. (2017) · Biomacromolecules · doi:10.1021/acs.biomac.7b00150 · Cited by 83 · see citing works
Applications of electrospun scaffolds with enlarged pores in tissue engineering
Zhang, Y., Zhang, M., Cheng, D., Xu, S., Du, C., Xie, L., & Zhao, W. (2022) · Biomaterials Science · doi:10.1039/d1bm01651b · Cited by 81 · see citing works
Banitaba, S. N., Ebadi, S. V., Salimi, P., Bagheri, A., Gupta, A., Arifeen, W. U., Chaudhary, V., Mishra, Y. K., Kaushik, A. K., & Mostafavi, E. (2022) · Materials Horizons · doi:10.1039/d2mh00879c · Cited by 80 · see citing works
Roohani‐Esfahani, S., Lu, Z., Li, J. J., Ellis-Behnke, R. G., Kaplan, D. L., & Zreiqat, H. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.10.009 · Cited by 74 · see citing works
Chakraborty, P. K., Adhikari, J., & Saha, P. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.10.216 · Cited by 74 · see citing works
Bidgoli, M. R., Alemzadeh, I., Tamjid, E., Khafaji, M., & Vossoughi, M. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.04.067 · Cited by 72 · see citing works
Wet electrospun silk fibroin/gold nanoparticle 3D matrices for wound healing applications
Aktürk, Ö., Kısmet, K., Yastı, A. Ç., Kuru, S., Duymuş, M. E., Kaya, F., Çaydere, M., Hücümenoğlu, S., & Keskin, D. S. (2016) · RSC Advances · doi:10.1039/c5ra24225h · Cited by 72 · see citing works
Electrospun scaffolds for bone tissue engineering
Martino, A. C. D., Liverani, L., Rainer, A., Salvatore, G., Trombetta, M., & Denaro, V. (2011) · MUSCULOSKELETAL SURGERY · doi:10.1007/s12306-011-0097-8 · Cited by 66 · see citing works
Silk fibroin for vascular regeneration
Wang, D., Liu, H., & Fan, Y. (2015) · Microscopy Research and Technique · doi:10.1002/jemt.22532 · Cited by 66 · see citing works
Zhang, L., Zhang, W., Hu, Y., Yang, F., Liu, H., Huang, Z., Wang, C., Ruan, D., Heng, B. C., Chen, W., et al. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c01716 · Cited by 62 · see citing works
Functional recovery guided by an electrospun silk fibroin conduit after sciatic nerve injury in rats
Park, S. Y., Ki, C. S., Park, Y. H., Lee, K. G., Kang, S. W., Kweon, H., & Kim, H. J. (2012) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1615 · Cited by 62 · see citing works
Self-citationKim, J. H., Park, C. H., Lee, O., Lee, J., Kim, J. W., Park, Y. H., & Ki, C. S. (2012) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.34274 · Cited by 61 · see citing works
Self-citationHuman dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol based silk scaffolds
Zhang, W., Ahluwalia, I. P., Literman, R., Kaplan, D. L., & Yelick, P. C. (2011) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.33062 · Cited by 61 · see citing works
Fabrication and evaluation of poly(epsilon‐caprolactone)/silk fibroin blend nanofibrous scaffold
Lim, J., Ki, C. S., Kim, J. W., Lee, K. G., Kang, S. W., Kweon, H., & Park, Y. H. (2011) · Biopolymers · doi:10.1002/bip.22016 · Cited by 59 · see citing works
Self-citationKim, H. H., Park, J. B., Kang, M. J., & Park, Y. H. (2014) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2014.06.052 · Cited by 58 · see citing works
Self-citationSilk fibroin‐based scaffolds for bone regeneration
Kuboyama, N., Kiba, H., Arai, K., Uchida, R., Tanimoto, Y., BHAWAL, U. K., Abiko, Y., Miyamoto, S., Knight, D. P., Asakura, T., et al. (2012) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.32839 · Cited by 52 · see citing works
Wang, C., Eswaramoorthy, R., Lin, T.-H., Chen, C., Fu, Y., Wang, C., Wu, S., Wang, G., Chang, J., & Ho, M. (2018) · Scientific Reports · doi:10.1038/s41598-018-28893-x · Cited by 52 · see citing works
Chung, D. E., & Um, I. C. (2014) · Fibers and Polymers · doi:10.1007/s12221-014-0153-8 · Cited by 49 · see citing works
Effect of molecular weight on electro-spinning performance of regenerated silk
Park, B. K., & Um, I. C. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.08.115 · Cited by 48 · see citing works
Park, J.-Y., Yang, C., Jung, I., Lim, H., Lee, J., Jung, U., Seo, Y., Park, J., & Choi, S. (2015) · Biomaterials Research · doi:10.1186/s40824-015-0027-1 · Cited by 48 · see citing works
Lee, J. H., Bae, Y. S., Kim, S. J., Song, D. W., Park, Y. H., Bae, D. G., Choi, J. H., & Um, I. C. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.07.179 · Cited by 47 · see citing works
Self-citationZhu, J., Du, Y. L., Backman, L. J., Chen, J., Ouyang, H., & Zhang, W. (2024) · Small · doi:10.1002/smll.202409739 · Cited by 46 · see citing works
Yang, X., Wang, X., Yu, F., Ma, L., Pan, X., Luo, G., Lin, S., Mo, X., He, C., & Wang, H. (2016) · RSC Advances · doi:10.1039/c6ra13713j · Cited by 46 · see citing works
Jaiswal, S., Dubey, A., & Debrupa Lahiri, A. (2019) · Journal of the Indian Institute of Science · doi:10.1007/s41745-019-00124-w · Cited by 45 · see citing works
Ki, C. S., Shih, H., & Lin, C. (2013) · Biomacromolecules · doi:10.1021/bm4004496 · Cited by 41 · see citing works
Self-citationCryopreservation of hMSCs seeded silk nanofibers based tissue engineered constructs
Bissoyi, A., Pramanik, K., Panda, N. N., & Sarangi, S. K. (2014) · Cryobiology · doi:10.1016/j.cryobiol.2014.04.008 · Cited by 40 · see citing works
Kim, M. K., Lee, J. Y., Oh, H.-J., Song, D. W., Kwak, H. W., Yun, H., Um, I. C., Park, Y. H., & Lee, K. H. (2015) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2015.05.040 · Cited by 40 · see citing works
Self-citationMembrane-reinforced three-dimensional electrospun silk fibroin scaffolds for bone tissue engineering
Yang, S. Y., Hwang, T. H., Che, L., Oh, J. S., Ha, Y., & Ryu, W. (2015) · Biomedical Materials · doi:10.1088/1748-6041/10/3/035011 · Cited by 38 · see citing works
Bioactive Nanostructured Scaffold-Based Approach for Tendon and Ligament Tissue Engineering
Govindaraju, D. T., Chen, C., Shalumon, K. T., Kao, H.-H., & Chen, J. (2023) · Nanomaterials · doi:10.3390/nano13121847 · Cited by 36 · see citing works
Electrospun fibrous silk fibroin/poly(L-lactic acid) scaffold for cartilage tissue engineering
Liu, W., Li, Z., Zheng, L., Zhang, X., Liu, P., Yang, T., & Han, B. (2016) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-016-9099-9 · Cited by 37 · see citing works
Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review
Zhu, S., Zhang, Q., Xu, X., Liu, Z., Cheng, G., Long, D., Cheng, L., & Dai, F. (2025) · Polymers · doi:10.3390/polym17060772 · Cited by 36 · see citing works
“…precursor cells (MC3T3-E1); moreover, the silk fibroin scaffolds were significantly superior to the PLA scaffolds in terms of the ability to form new bone in the animal experiments [75], which suggests that electrostatically spun silk fibroin scaffolds have good potential for bone tissue repair.”
Cha, B. J., Kwak, H. W., Park, A. R., Kim, S. H., Park, S., Kim, H. J., Kim, I. S., Lee, K. H., & Park, Y. H. (2013) · Biopolymers · doi:10.1002/bip.22359 · Cited by 34 · see citing works
Self-citationBiagiotti, M., Bassani, G. A., Chiarini, A., Vincoli, V., Prà, I. D., Cosentino, C., Alessandrino, A., Taddei, P., & Freddi, G. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.833157 · Cited by 33 · see citing works
Mann, G. S., Singh, L. P., Kumar, P., Singh, S., & Prakash, C. (2019) · Journal of Thermoplastic Composite Materials · doi:10.1177/0892705719856052 · Cited by 32 · see citing works
Gang, E. H., Ki, C. S., Kim, J. W., Lee, J., Gyu, B., Lee, K. H., & Park, Y. H. (2012) · Fibers and Polymers · doi:10.1007/s12221-012-0685-8 · Cited by 30 · see citing works
Self-citationChiarini, A., Freddi, G., Liu, D., Armato, U., & Prà, I. D. (2016) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0124 · Cited by 28 · see citing works
Li, Y., Liu, Z., Tang, Y., Fan, Q., Feng, W., Luo, C., Dai, G., Ge, Z., Zhang, J., Zou, G., et al. (2020) · Acta Biochimica et Biophysica Sinica · doi:10.1093/abbs/gmaa042 · Cited by 27 · see citing works
3D Printing Strategies for Precise and Functional Assembly of Silk-based Biomaterials
Cui, X., Zhang, J., Yan, Q., Chang, S., Allardyce, B. J., Rajkhowa, R., Wang, H., & Zhang, K. (2024) · Engineering · doi:10.1016/j.eng.2023.09.022 · Cited by 27 · see citing works
Alam, H., Dalgıç, A. D., Tezcaner, A., Özen, C., & Keskin, D. S. (2019) · International Journal of Polymeric Materials · doi:10.1080/00914037.2019.1581198 · Cited by 26 · see citing works
Zhu, J., Luo, J., Zhao, X., Gao, J., & Xiong, J. (2016) · Journal of Biomaterials Applications · doi:10.1177/0885328216659775 · Cited by 25 · see citing works
Effect of plasma-irradiated silk fibroin in bone regeneration
Uchida, R., BHAWAL, U. K., Kiba, H., Arai, K., Tanimoto, Y., Kuboyama, N., Asakura, T., & Nishiyama, N. (2014) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2014.02.016 · Cited by 24 · see citing works
Characterization of Electrospun Silk Fibroin Scaffolds for Bone Tissue Engineering: A Review
Suaza, M. L. M., Moncada, M. E., & Orozco, C. P. O. (2020) · TecnoLógicas · doi:10.22430/22565337.1573 · Cited by 21 · see citing works
Hu, C.-S., Li, X., Huang, Z., Li, J., Wang, J., Pu, X., Li, Y., Wu, J., & Yin, G. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.151171 · Cited by 21 · see citing works
Taddei, P., Tozzi, S., Zuccheri, G., Martinotti, S., Ranzato, E., Chiono, V., Carmagnola, I., & Tsukada, M. (2016) · Materials Science and Engineering C · doi:10.1016/j.msec.2016.09.055 · Cited by 19 · see citing works
Tao, G., Liu, L., Wang, Y., Chang, H., Zhao, P., Zuo, H., & He, H. (2016) · Journal of Nanomaterials · doi:10.1155/2016/9505704 · Cited by 17 · see citing works
Nanofiber composites in bone tissue engineering
Liverani, L., Roether, J. A., & Boccaccini, A. R. (2017) · Elsevier eBooks · doi:10.1016/b978-0-08-100173-8.00012-0 · Cited by 17 · see citing works
Manufacturing processes for polymeric micro and nanoparticles and their biomedical applications
Braz, A. L., & Ahmed, I. (2017) · AIMS bioengineering · doi:10.3934/bioeng.2017.1.46 · Cited by 17 · see citing works
The Application of Regenerated Silk Fibroin in Tissue Repair
Li, Z., Tan, G., Xie, H., & Lu, S. (2024) · Materials · doi:10.3390/ma17163924 · Cited by 16 · see citing works
Degradation behaviour of magnesium alloy and its composite used as a biomaterial
Jhamb, S. K., Goyal, A., Pandey, A., Marwaha, J., & Matai, J. (2021) · E3S Web of Conferences · doi:10.1051/e3sconf/202130901085 · Cited by 13 · see citing works
Senthil, R., & Kavukcu, S. B. (2022) · Materials Chemistry and Physics · doi:10.1016/j.matchemphys.2022.126444 · Cited by 11 · see citing works
Baudequin, T., Bédoui, F., Dufresne, M., Paullier, P., & Legallais, C. (2015) · Tissue Engineering Part A · doi:10.1089/ten.tea.2014.0580 · Cited by 11 · see citing works
Instructive proteins for tissue regeneration
Guarino, V., Benfenati, V., Maya, I. C., Saracino, E., Zamboni, R., & Ambrosio, L. (2017) · Elsevier eBooks · doi:10.1016/b978-0-08-100979-6.00002-1 · Cited by 11 · see citing works
Breslauer, D. N., & Kaplan, D. L. (2012) · Elsevier eBooks · doi:10.1016/b978-0-444-53349-4.00233-8 · Cited by 11 · see citing works
Modeling Analysis of Silk Fibroin/Poly(ε-caprolactone) Nanofibrous Membrane under Uniaxial Tension
Yin, Y., Zhao, X., & Xiong, J. (2019) · Nanomaterials · doi:10.3390/nano9081149 · Cited by 11 · see citing works
Silk fibroin and potential uses in regenerative dentistry - a systematic review
Vîrlan, M. J. R., Calenic, B., Zaharia, C., & Greabu, M. (2014) · STOMATOLOGY EDU JOURNAL · doi:10.25241/stomaeduj.2014.1(2).art.5 · Cited by 10 · see citing works
Advancements in synthetic polymers for 3D bioprinting materials, applications, and future prospects
Agarwalla, A., Ahmed, W. K., Al-Marzouqi, A. H., Zaneldin, E. K., Rizvi, T. A., & Khan, M. (2025) · International Journal of Polymeric Materials · doi:10.1080/00914037.2025.2562081 · Cited by 11 · see citing works
Effect of the surface morphology of silk fibroin scaffolds for bone regeneration
BHAWAL, U. K., Uchida, R., Kuboyama, N., Asakura, T., Hiratsuka, K., & Nishiyama, N. (2016) · Bio-Medical Materials and Engineering · doi:10.3233/bme-161595 · Cited by 9 · see citing works
Flexure-Based Device for Cyclic Strain-Mediated Osteogenic Differentiation
Kang, K. S., Jeong, Y. H., Hong, J. M., Yong, W.-J., Rhie, J., & Cho, D. (2013) · Journal of Biomechanical Engineering · doi:10.1115/1.4025103 · Cited by 9 · see citing works
Electrospun nanofibrous tissue scaffolds
Chae, T., & Ko, F. (2016) · Elsevier eBooks · doi:10.1016/b978-0-08-100907-9.00019-2 · Cited by 9 · see citing works
A step toward engineering thick tissues: Distributing microfibers within 3D printed frames
Molde, J., Steele, J. A. M., Pastino, A. K., Mahat, A., Murthy, N. S., & Kohn, J. (2019) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36838 · Cited by 9 · see citing works
Advances in Tissue Engineering and Regeneration
Ghosal, K., Sarkar, P., Saha, R., Ghosh, S., & Sarkar, K. (2020) · Springer eBooks · doi:10.1007/978-3-030-34471-9_22 · Cited by 8 · see citing works
3D Electrospun Fibrous Structures from Biopolymers
Xu, H., & Yang, Y. (2014) · ACS symposium series · doi:10.1021/bk-2014-1175.ch007 · Cited by 8 · see citing works
Biomaterials and scaffolds for the treatment of spinal cord injury
Wen, X., Saijilafu, Luo, Z., Yang, H., Wang, W., & Yang, L. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-813477-1.00006-2 · Cited by 8 · see citing works
Poly (vinyl alcohol)/Silk Fibroin/Ag NPs composite nanofibers for bone tissue engineering
Mejía, M. L., Moncada, M. E., & Orozco, C. P. O. (2021) · Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) · doi:10.1109/embc46164.2021.9629992 · Cited by 7 · see citing works
Silk fibroin microfiber and nanofiber scaffolds for tissue engineering and regeneration
Freddi, G. (2014) · Elsevier eBooks · doi:10.1533/9780857097064.1.157 · Cited by 7 · see citing works
Synthetic Multi-level Matrices for Bone Regeneration
Boyd, N. R., Boyd, R. L., Simon, G. P., & Nisbet, D. R. (2011) · Humana Press eBooks · doi:10.1007/978-1-61779-322-6_6 · Cited by 7 · see citing works
Shahid, A., Moeen, F., Habib, S., Arshad, A., Zeeshan, R., Chaudhry, A. A., Khalid, H., Alhamoudi, F. H., Akhtar, H., & Khan, A. F. (2024) · Materials Chemistry and Physics · doi:10.1016/j.matchemphys.2024.129911 · Cited by 6 · see citing works
In vitro models of muscle spindles: From traditional methods to 3D bioprinting strategies
Kang, Y., Kalaskar, D. M., & Player, D. J. (2025) · Journal of Tissue Engineering · doi:10.1177/20417314251343388 · Cited by 5 · see citing works
Hakimi, O., Vollrath, F. W., & Carr, A. J. (2011) · Elsevier eBooks · doi:10.1016/b978-0-08-088504-9.00435-9 · Cited by 5 · see citing works
Biomimetic Tissue Engineering for Musculoskeletal Tissues
Seale, N. M., Zeng, Y., & Varghese, S. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-811467-4.00009-7 · Cited by 5 · see citing works
Yadav, M., Paritosh, K., Pareek, N., & Vivekanand, V. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-816901-8.00009-2 · Cited by 5 · see citing works
Fibrous scaffolds for bone tissue engineering
Moy, J., Limaye, A., & Arinzeh, T. L. (2020) · Elsevier eBooks · doi:10.1016/b978-0-08-102850-6.00015-2 · Cited by 5 · see citing works
Chen, L., Huang, Y., Yang, R., Xiao, J., Gao, J., Zhang, D., Cao, D., & Xiao, K. (2021) · Bioengineered · doi:10.1080/21655979.2021.1982270 · Cited by 5 · see citing works
- Authors
- Park, S. Y., Ki, C. S., Park, Y. H., Jung, H. M., Woo, K. M., & Kim, H. J.
- Affiliations
- Seoul National University (KR)
- Corresponding author
- Kim, Hyun Jeong
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2010-04
- Volume
- 16
- Issue
- 4
- Pages
- 1271-1279
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- OpenAlex
- W2066288600
- Cited by
- 120
- References
- 44
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 5 · 2023: 2 · 2022: 6 · 2021: 7 · 2020: 8 · 2019: 11 · 2018: 7 · 2017: 9 · 2016: 12 · 2015: 10 · 2014: 11 · 2013: 5 · 2012: 7
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Tissue Engineering Part A (publishedVersion)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Funders
- Korea Science and Engineering Foundation
- European Social Fund
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- silk fibroin, electrospun scaffolds, macropores, bone regeneration, bone substitutes, polylactic acid scaffolds, osteoblast proliferation, alkaline phosphatase activity, focal adhesion kinase, critical-size calvarial defect, new bone formation, pore size control
- MeSH terms
- Animals, Biocompatible Materials, Bone Regeneration, Fibroins, Male, Materials Testing, Microscopy, Electron, Scanning, Polyesters, Polymers, Radiography, Bombyx, Skull, 3T3 Cells, Rats, Sprague-Dawley, Lactic Acid, Tissue Engineering, Mice, Rats, Tissue Scaffolds
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
