Diazeniumdiolate-doped poly(lactic-co-glycolic acid)-based nitric oxide releasing films as antibiofilm coatings
Open accessAbstract
Nitric oxide (NO) releasing films with a bilayer configuration are fabricated by doping dibutyhexyldiamine diazeniumdiolate (DBHD/N2O2) in a poly(lactic-co-glycolic acid) (PLGA) layer and further encapsulating this base layer with a silicone rubber top coating. By incorporating pH sensitive dyes within the films, pH changes in the PLGA layer are visualized and correlated with the NO release profiles (flux vs. time). It is demonstrated that PLGA acts as both a promoter and controller of NO release from the coating by providing protons through its intrinsic acid residues (both end groups and monomeric acid impurities) and hydrolysis products (lactic acid and glycolic acid). Control of the pH changes within the PLGA layer can be achieved by adjusting the ratio of DBHD/N2O2 and utilizing PLGAs with different hydrolysis rates. Coatings with a variety of NO release profiles are prepared with lifetimes of up to 15 d at room temperature (23 °C) and 10 d at 37 °C. When incubated in a CDC flow bioreactor for a one week period at RT or 37 °C, all the NO releasing films exhibit considerable antibiofilm properties against gram-positive Staphylococcus aureus and gram-negative Escherichia coli. In particular, compared to the silicone rubber surface alone, an NO releasing film with a base layer of 30 wt% DBHD/N2O2 mixed with poly(lactic acid) exhibits an ∼98.4% reduction in biofilm biomass of S. aureus and ∼99.9% reduction for E. coli at 37 °C. The new diazeniumdiolate-doped PLGA-based NO releasing coatings are expected to be useful antibiofilm coatings for a variety of indwelling biomedical devices (e.g., catheters).
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Handa et al. and Cai et al. studied PLGA additives with different hydrolysis rates to slowly generate protons in the organic polymer phase and drive NO release from NONOates.
“Therefore, Handa et al.154,155 as well as Cai et al.156 studied various poly(lactic-co-glycolic acid) (PLGA) additives with different hydrolysis rates as a means to slowly produce protons within the organic polymer phase that can continue to drive NO release reaction from NONOates.”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [156]
Cai et al. fabricated NO releasing silicone rubber films containing DBHD/N2O2 and PLGA additives that can release NO for 10 d under physiological conditions.
“Cai et al. fabricated NO releasing silicone rubber films containing DBHD/N2O2 along with PLGA (RG 502H) additives that can release NO for 10 d at physiological conditions.”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [156]
The NO release films exhibited substantial antibiofilm properties against S. aureus (98.4% reduction) and E. coli (99.9% reduction) compared with controls.
“The NO release films exhibited substantial antibiofilm properties against both gram-positive S. aureus (98.4 % reduction) and gram-negative E. coli (99.9% reduction) when compared to controls.156”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [156]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A Switchable Biocompatible Polymer Surface with Self‐Sterilizing and Nonfouling Capabilities
Cheng, G., Xue, H., Zhang, Z., Chen, S., & Jiang, S. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200803570 · Cited by 371 · see citing works
Frost, M. C., Reynolds, M. M., & Meyerhoff, M. E. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2004.06.006 · Cited by 343 · see citing works
Self-citationBarraud, N., Storey, M. V., Moore, Z. P., Webb, J. S., Rice, S. A., & Kjelleberg, S. (2009) · Microbial Biotechnology · doi:10.1111/j.1751-7915.2009.00098.x · Cited by 289 · see citing works
Privett, B. J., Nutz, S. T., & Schoenfisch, M. H. (2010) · Biofouling · doi:10.1080/08927014.2010.534552 · Cited by 45 · see citing works
Yan, Q., Major, T. C., Bartlett, R. H., & Meyerhoff, M. E. (2011) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2011.04.026 · Cited by 41 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Wo, Y., Brisbois, E. J., Bartlett, R. H., & Meyerhoff, M. E. (2016) · Biomaterials Science · doi:10.1039/c6bm00271d · Cited by 251 · see citing works
Self-citationBrisbois, E. J., Davis, R. P., Jones, A. M., Major, T. C., Bartlett, R. H., Meyerhoff, M. E., & Handa, H. (2015) · Journal of Materials Chemistry B · doi:10.1039/c4tb01839g · Cited by 101 · see citing works
Self-citationColletta, A., Wu, J., Wo, Y., Kappler, M., Chen, H., Xi, C., & Meyerhoff, M. E. (2015) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.5b00032 · Cited by 89 · see citing works
Self-citationHanda, H., Brisbois, E. J., Major, T. C., Refahiyat, L., Amoako, K. A., Annich, G. M., Bartlett, R. H., & Meyerhoff, M. E. (2013) · Journal of Materials Chemistry B · doi:10.1039/c3tb20277a · Cited by 64 · see citing works
Self-citationHanda, H., Major, T. C., Brisbois, E. J., Amoako, K. A., Meyerhoff, M. E., & Bartlett, R. H. (2014) · J. Mater. Chem. B · doi:10.1039/c3tb21771j · Cited by 64 · see citing works
Self-citationThromboresistant/anti-biofilm catheters via electrochemically modulated nitric oxide release
Ren, H., Colletta, A., Koley, D., Wu, J., Xi, C., Major, T. C., Bartlett, R. H., & Meyerhoff, M. E. (2015) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2014.12.003 · Cited by 55 · see citing works
Self-citationWonoputri, V., Gunawan, C., Liu, S., Barraud, N., Yee, L. H., Lim, M., & Amal, R. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b07971 · Cited by 38 · see citing works
Peng, Z., Ao, H., Wang, L., Guo, S., & Tang, T. (2015) · RSC Advances · doi:10.1039/c5ra07540h · Cited by 31 · see citing works
- Authors
- Cai, W., Wu, J., Xi, C., & Meyerhoff, M. E.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Xi, Chuanwu
- Meyerhoff, Mark E.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2012-11
- Volume
- 33
- Issue
- 32
- Pages
- 7933-7944
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC3432167
- OpenAlex
- W1991642756
- Cited by
- 77
- References
- 5
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 1 · 2023: 4 · 2022: 7 · 2021: 5 · 2020: 6 · 2019: 8 · 2018: 5 · 2017: 9 · 2016: 7 · 2015: 9 · 2014: 7 · 2013: 2
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (EB-000783, EB-004527)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Keywords
- diazeniumdiolates, poly(lactic-co-glycolic acid), antibiofilm coatings, silicone rubber, pH-sensitive dyes, Staphylococcus aureus, Escherichia coli, biofilm inhibition, catheters
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Anti-Bacterial Agents, Azo Compounds, Escherichia coli, Escherichia coli Infections, Humans, Hydrogen-Ion Concentration, Nitric Oxide, Polyglycolic Acid, Silicone Elastomers, Staphylococcal Infections, Staphylococcus aureus, Prosthesis-Related Infections, Biofilms, Lactic Acid, Coated Materials, Biocompatible
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
