Thromboresistant/anti-biofilm catheters via electrochemically modulated nitric oxide release
Open accessAbstract
Inexpensive nitric oxide (NO) release strategies to prevent thrombosis and bacterial infections are desirable for implantable medical devices. Herein, we demonstrate the utility of electrochemically modulated NO release from a catheter model using an inner copper wire working electrode and an inorganic nitrite salt solution reservoir. These catheters generate NO surface fluxes of >1.0 × 10(-10)mol min(-1) cm(-2) for more than 60 h. Catheters with an NO flux of 1.1 × 10(-10)mol min(-1) cm(-2) are shown to significantly reduce surface thrombus formation when implanted in rabbit veins for 7h. Further, the ability of these catheters to exhibit anti-biofilm properties against bacterial species commonly causing bloodstream and urinary catheter infections is examined. Catheters releasing NO continuously during the 2d growth of Staphylococcus aureus exhibit a 6 log-unit reduction in viable surface bacteria. We also demonstrate that catheters generating NO for only 3h at a flux of 1.0 × 10(-10)mol min(-1) cm(-2) lower the live bacterial counts of both 2d and 4d pre-formed Escherichia coli biofilms by >99.9%. Overall, the new electrochemical NO-release devices could provide a cost-effective strategy to greatly enhance the biocompatibility and antimicrobial properties of intravascular and urinary catheters, as well as other implantable medical devices.
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Nitrite can generate NO via electrochemical reduction at an electrode surface using Cu(I) from Cu0 oxidation or Cu(II)-ligand complexes mimicking nitrite reductase.
“Recently, it was demonstrated that nitrite can also be used to generate NO via electrochemical reduction at an electrode surface with either Cu(I) ion generated from oxidation of Cu0145,146 or via the use of Cu(II)-ligand complexes that mimic the active site of nitrite reductase (e.g., Cu(II)-tri(2-pyridylmethyl)amine, Cu(II)TPMA).147”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [146]
NO releasing or generating polymers can have strong bactericidal effects, even for bacteria able to metabolize and deactivate NO, such as P. aeruginosa with NO reductase.
“It has been clearly demonstrated that NO releasing/generating polymers can have strong bactericidal effects,19,146,147,162,201,227,283,284 even for bacteria that are able to metabolize and deactivate NO, such as P. aeruginosa, that possesses NO reductase enzyme that converts NO to nitrous oxide (N2O) and ultimately nitrogen (N2).86,201,219”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [146]
Electrochemical NO generating catheters using an inner copper wire working electrode and inorganic nitrite salt solution can generate NO through catheter walls with a flux > 1.0 × 10−10 mol cm−2 min−1 for more than 60 h.
“Electrochemical NO generating catheters using inner copper wire working electrode and inorganic nitrite salt solution can generate NO through the walls of the catheters with a flux > 1.0 × 10−10 mol cm−2 min−1 for more than 60 h.146”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [146]
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- [25]Jourd'heuil D Jourd'heuil FL Kutchukian PS Musah RA Wink DA Grisham MB J. Biol. Chem 2001 276 28799 11373284
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Self-citation
- Authors
- Ren, H., Colletta, A., Koley, D., Wu, J., Xi, C., Major, T. C., Bartlett, R. H., & Meyerhoff, M. E.
- Affiliations
- University of Michigan (US)
- Oregon State University (US)
- Corresponding author
- Meyerhoff, Mark E.
- ORCID
- Venue
- Bioelectrochemistry
- Publisher
- Elsevier BV
- Published
- 2015-08
- Volume
- 104
- Pages
- 10-16
- ISSN
- 1567-5394 (ISSN-L)
- PMC
- PMC4480771
- OpenAlex
- W2041861222
- Cited by
- 55
- References
- 48
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 6 · 2020: 6 · 2019: 4 · 2018: 7 · 2017: 5 · 2016: 13 · 2015: 3
- FWCI
- 4.65
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Bioelectrochemistry (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (NIH-EB-000783, NIH-R56-HL-119403-01)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Keywords
- nitric oxide release, Staphylococcus aureus, Escherichia coli biofilm, biomedical implants, biocompatibility
- MeSH terms
- Animals, Electrochemistry, Nitric Oxide, Rabbits, Staphylococcus aureus, Thrombosis, Biofilms, Escherichia coli K12, Catheter-Related Infections, Catheters
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