The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering
Open accessAbstract
Approximately 285 million people worldwide suffer from diabetes, with insulin supplementation as the most common treatment measure. Regenerative medicine approaches such as a bioengineered pancreas has been proposed as potential therapeutic alternatives. A bioengineered pancreas will benefit from the development of a bioscaffold that supports and enhances cellular function and tissue development. Perfusion-decellularized organs are a likely candidate for use in such scaffolds since they mimic compositional, architectural and biomechanical nature of a native organ. In this study, we investigate perfusion-decellularization of whole pancreas and the feasibility to recellularize the whole pancreas scaffold with pancreatic cell types. Our result demonstrates that perfusion-decellularization of whole pancreas effectively removes cellular and nuclear material while retaining intricate three-dimensional microarchitecture with perfusable vasculature and ductal network and crucial extracellular matrix (ECM) components. To mimic pancreatic cell composition, we recellularized the whole pancreas scaffold with acinar and beta cell lines and cultured up to 5 days. Our result shows successful cellular engraftment within the decellularized pancreas, and the resulting graft gave rise to strong up-regulation of insulin gene expression. These findings support biological utility of whole pancreas ECM as a biomaterials scaffold for supporting and enhancing pancreatic cell functionality and represent a step toward bioengineered pancreas using regenerative medicine approaches.
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Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
Brown, B. N., Valentin, J. E., Stewart-Akers, A. M., McCabe, G. P., & Badylak, S. F. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2008.11.040 · Cited by 896 · cited 1×
“This requirement is crucial because residual DNA fragments in decellularized ECM have shown to lead to cytocompatibility issues in vitro and adverse immunological response upon implantation [49,60,61].”
Self-citationRegeneration and experimental orthotopic transplantation of a bioengineered kidney
Song, J. J., Guyette, J. P., Gilpin, S. E., Gonzalez, G., Vacanti, J. P., & Ott, H. C. (2013) · Nature Medicine · doi:10.1038/nm.3154 · Cited by 793
The use of whole organ decellularization for the generation of a vascularized liver organoid
Baptista, P. M., Siddiqui, M. M., Lozier, G., Rodriguez, S. R., Atala, A., & Soker, S. (2011) · Hepatology · doi:10.1002/hep.24067 · Cited by 681
Organ engineering based on decellularized matrix scaffolds
Song, J. J., & Ott, H. C. (2011) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2011.03.005 · Cited by 501
Sullivan, D. C., Mirmalek-Sani, S.-H., Deegan, D. B., Baptista, P. M., Aboushwareb, T., Atala, A., & Yoo, J. J. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.07.023 · Cited by 363
A Whole-Organ Regenerative Medicine Approach for Liver Replacement
Soto-Gutierrez, A., Zhang, L., Medberry, C., Fukumitsu, K., Faulk, D., Jiang, H., Reing, J., Gramignoli, R., Komori, J., Ross, M., et al. (2011) · Tissue Engineering Part C: Methods · doi:10.1089/ten.tec.2010.0698 · Cited by 311
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Cited by
Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications
Gilpin, A., & Yang, Y. (2017) · BioMed Research International · doi:10.1155/2017/9831534 · Cited by 742
A decade of progress in tissue engineering
Khademhosseini, A., & Langer, R. (2016) · Nature Protocols · doi:10.1038/nprot.2016.123 · Cited by 747
Extracellular matrix-based biomaterial scaffolds and the host response
Aamodt, J. M., & Grainger, D. W. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.02.003 · Cited by 509
Perfusion decellularization of whole organs
Guyette, J. P., Gilpin, S. E., Charest, J. M., Tapias, L. F., Ren, X., & Ott, H. C. (2014) · Nature Protocols · doi:10.1038/nprot.2014.097 · Cited by 275
Sackett, S. D., Tremmel, D. M., Ma, F., Feeney, A. K., Maguire, R. M., Brown, M. E., Zhou, Y., Li, X., O’Brien, C., Li, L., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-28857-1 · Cited by 269
Metadata
- Authors
- Goh, S.-K., Bertera, S., Olsen, P., Candiello, J. E., Halfter, W., Uechi, G., Balasubramani, M., Johnson, S. A., Sicari, B. M., Kollar, E., Badylak, S. F., & Banerjee, I.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 34
- Issue
- 28
- Pages
- 6760-6772
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2079312215
- Cited by
- 271
- References
- 9
- FWCI
- 17.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Goh, S.-K., Bertera, S., Olsen, P., Candiello, J. E., Halfter, W., Uechi, G., Balasubramani, M., Johnson, S. A., Sicari, B. M., Kollar, E., Badylak, S. F., & Banerjee, I. (2013). Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering. Biomaterials. https://doi.org/10.1016/j.biomaterials.2013.05.066
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