Cited by 208RNA Interference and Gene DeliveryRNA and protein synthesis mechanismsRNA Research and Splicing

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Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine

Kwon, Hyokyoung, Kim, Minjeong, Seo, Yunmi, Moon, Yae Seul, Lee, Hwa Jeong, Lee, Kyuri, Lee, Hyukjin·2018·Biomaterials

DOI 10.1016/j.biomaterials.2017.11.034

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Abstract

The field of gene therapy has evolved over the past two decades after the first introduction of nucleic acid drugs, such as plasmid DNA (pDNA). With the development of in vitro transcription (IVT) methods, synthetic mRNA has become an emerging class of gene therapy. IVT mRNA has several advantages over conventional pDNA for the expression of target proteins. mRNA does not require nuclear localization to mediate protein translation. The intracellular process for protein expression is much simpler and there is no potential risk of insertion mutagenesis. Having these advantages, the level of protein expression is far enhanced as comparable to that of viral expression systems. This makes IVT mRNA a powerful alternative gene expression system for various applications in regenerative medicine. In this review, we highlight the synthesis and preparation of IVT mRNA and its therapeutic applications. The article includes the design and preparation of IVT mRNA, chemical modification of IVT mRNA, and therapeutic applications of IVT mRNA in cellular reprogramming, stem cell engineering, and protein replacement therapy. Finally, future perspectives and challenges of IVT mRNA are discussed.

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Authors: Kwon, H., Kim, M., Seo, Y., Moon, Y. S., Lee, H. J., Lee, K., & Lee, H.
Affiliations: Ewha Womans University (KR)
Corresponding author: Lee, Kyuri
Lee, Hyukjin
ORCID: Kim, Minjeong
Lee, Hwa Jeong
Venue: Biomaterials
Publisher: Elsevier BV
Published: 2018-02
Volume: 156
Pages: 172-193
ISSN: 0142-9612 (ISSN-L)
DOI: 10.1016/j.biomaterials.2017.11.034
OpenAlex: W2769785887
Cited by: 208
References: 257
Citations by year: 2026: 17 · 2025: 40 · 2024: 32 · 2023: 43 · 2022: 30 · 2021: 19 · 2020: 14 · 2019: 9 · 2018: 4
FWCI: 0.69
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Biomaterials (publishedVersion)
PubMed (publishedVersion)
Funders: National Research Foundation of Korea
Ministry of Science, ICT and Future Planning (2014M3C1A3054153)
GiRC Program (2012K1A1A2A01056092)
Basic Science Research Program (2015R1A1A1A05027352)
BioMedical Technology Development Program (2015M3A9D7031026)
Ministry of Education (2017R1D1A1B03028278)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords: synthetic mRNA, in vitro transcription, regenerative medicine, mRNA design, cellular reprogramming, stem cell engineering, protein replacement therapy, nucleic acid therapeutics
MeSH terms: Animals, DNA, Humans, Plasmids, RNA, Messenger, Stem Cells, Regenerative Medicine, Cell Engineering

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Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine

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