The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.
Vascularized cancer on a chip: The effect of perfusion on growth and drug delivery of tumor spheroid
Open accessAbstract
Tumor vasculature creates a hostile tumor microenvironment (TME) in vivo and nourishes cancers, resulting in cancer progression and drug resistance. To mimic the biochemical and biomechanical environments of tumors in vitro, several models integrated with a vascular network have been reported. However, the tumor responses to biochemical and biomechanical stimuli were evaluated under static conditions and failed to incorporate the effects of blood flow to tumors. In this study, we present a tumor-on-a-chip platform that enables the evaluation of tumor activities with intraluminal flow in an engineered tumor vascular network. The fibroblasts in the tumor spheroid induced angiogenic sprouts, which constructed a perfusable vascular network in a tumor spheroid. The perfusability of the engineered vascular network was preserved during the culture. Moreover, perfusion for over 24 h significantly increased the proliferation activities of tumor cells and decreased cell death in the spheroid. Drug administration under perfusion condition did not show the dose-dependent effects of anticancer drugs on tumor activities in contrast to the results under static conditions. Our results demonstrate the importance of flow in a vascular network for the evaluation of tumor activities in a drug screening platform.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“the vascularization of tumoroids can be maintained for 22 days.”
From 3D Biomimetic Models to Reconstitute Tumor Microenvironment In Vitro: Spheroids, Organoids, and Tumor‐on‐a‐Chip (2023) · cites this paper as [104]
In the citing paper
“Sprouting from the endothelial layer in the side channels into the middle compartment containing a tumor spheroid was triggered by the presence of fibroblasts in the spheroids [58].”
From Cancer-on-chip models for metastasis: importance of the tumor microenvironment (2024) · cites this paper as [58]
Citations
Cites
Organogenesis in a dish: Modeling development and disease using organoid technologies
Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931
Influence of tumour micro-environment heterogeneity on therapeutic response
Junttila, M. R., & de Sauvage, F. J. (2013) · Nature · doi:10.1038/nature12626 · Cited by 2,694
Cell and environment interactions in tumor microregions: the multicell spheroid model.
Sutherland, R. M. (1988) · Science · doi:10.1126/science.2451290 · Cited by 1,803
Spheroid-based drug screen: Considerations and practical approach
Friedrich, J., Seidel, C., Ebner, R., & Kunz-Schughart, L. A. (2009) · Nature Protocols · doi:10.1038/nprot.2008.226 · Cited by 1,742
Role of tumor microenvironment in tumorigenesis
Wang, M., Zhao, J., Zhang, L., Wei, F., Lian, Y., Wu, Y., Gong, Z., Zhang, S., Zhou, J., Cao, K., et al. (2017) · Journal of Cancer · doi:10.7150/jca.17648 · Cited by 1,404
Cell surface mechanics and the control of cell shape, tissue patterns and morphogenesis
Lecuit, T., & Lenne, P. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2222 · Cited by 1,326
Abbott, A. (2003) · Nature · doi:10.1038/424870a · Cited by 1,228
Spherical Cancer Models in Tumor Biology
Weiswald, L., Bellet, D. H., & Dangles‐Marie, V. (2015) · Neoplasia · doi:10.1016/j.neo.2014.12.004 · Cited by 1,194
Reconstruction of Tissues by Dissociated Cells
Steinberg, M. S. (1963) · Science · doi:10.1126/science.141.3579.401 · Cited by 1,127
The Role of Mechanical Forces in Tumor Growth and Therapy
Jain, R. K., Martin, J. D., & Stylianopoulos, T. (2014) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071813-105259 · Cited by 1,044
Cited by
Sorted by citations
Items 301–331 of 331
Kamei, K. (2024) · The Journal of the Institute of Electrical Engineers of Japan · doi:10.1541/ieejjournal.144.189
Human organs-on-a-chip for cancer modeling
Hegde, M., Girisa, S., & Harsha, C. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13782-2.00005-x
Polysaccharide-Based Hydrogels for Bioengineering 3D Tumor Models
Magalhães, M. V., Coutinho, I., Barrias, C. C., & Bidarra, S. J. (2024) · Springer eBooks · doi:10.1007/978-3-031-56363-8_22
4D Evaluation Approach for Cell Signaling Activity in Multicellular Tumor Spheroids
Tsutsumi, M., Yamaguchi, K., & Nemoto, T. (2024) · Nanomedicine and nanotoxicology · doi:10.1007/978-981-97-5288-1_15
Design patterns of tumor-on-chips utilizing fluidic devices and their latest trends
Mori, N., & Kida, Y. S. (2024) · Drug Delivery System · doi:10.2745/dds.39.184
Huang, Z., Tsun, Y. M., Liang, C., Wu, Z., Aurich, T., Liu, L., Sugimura, R., & Lee, S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.17.628981
How far have we come in our efforts to apply personalized therapy for lung cancer at bedside?
Kim, H. S., Kim, I. K., Lee, S. B., You, S.-H., Yeo, C. D., Kim, J.-A., Chung, S. H., Lee, S. H., & Kim, H. J. (2024) · Molecular & Cellular Toxicology · doi:10.1007/s13273-024-00500-3
Shah, S., Osuala, K. O., Brock, E. J., Ji, K., Sloane, B. F., & Mattingly, R. R. (2025) · Cells · doi:10.3390/cells14030220
Drugs and cellular dynamics in tumor microenvironment using microphysiological systems
Nashimoto, Y. (2025) · Folia Pharmacologica Japonica · doi:10.1254/fpj.24063
Nam, K., Kim, H., Ravichandran, N. K., Park, J., Kim, G., Yeo, W.-J., Jeon, J. S., Kang, K. S., & Lee, K. (2025) · SSRN Electronic Journal · doi:10.2139/ssrn.5338380
An Intelligent Magneto‐Mechanical Platform for Cellular Sensing in 3D Microenvironments
Quan, Y., Wang, Y., Ding, S., Zhou, B., & Zhou, Y. (2025) · Advanced Science · doi:10.1002/advs.202519132
Appuhami, K., Nakamura-Norimoto, A., & Kida, Y. S. (2026) · Micromachines · doi:10.3390/mi17020171
Targeting the cell cycle : A novel therapeutic strategy against ATIP3- deficient breast cancer
Haykal, M. M. (2023) · theses.fr (ABES) · doi:10.70675/03dee32bz395cz469ez9a0dzf4c5f2e84330
Delaney, W. H., Faulkner, S. A., Lam, W. A., Boulis, N. M., Qiu, Y., & Lei, K. (2026) · Materials Today Advances · doi:10.1016/j.mtadv.2026.100770
Xu, Y., Wubuli, N., & Zhang, J. (2026) · Stem Cell Reviews and Reports · doi:10.1007/s12015-026-11145-1
Microfluidic Technology in Cancer Treatment Current Applications and Future Potentials
Cai, X. (2026) · Highlights in Science Engineering and Technology · doi:10.54097/269rcd65
In vitro head and neck cancer models for photodynamic and photothermal therapies
Egloff‐Juras, C., Meyer, M., Bezdetnaya, L., Phulpin, B., Dolivet, G., & Lassalle, H. (2026) · SLAS TECHNOLOGY · doi:10.1016/j.slast.2026.100435
Douglas, Y. K., & Adham, S. A. (2026) · Journal of Translational Medicine · doi:10.1186/s12967-026-08763-1
Li, Y., Yang, Y., Zhao, L., Bian, Z., & Zhang, Z. (2026) · Bioengineering · doi:10.3390/bioengineering13080947
Cretti, G., Ballerini, M., Mantegazza, A., Occhetta, P., & Rasponi, M. (2026) · Advanced Materials Technologies · doi:10.1002/admt.71277
Cell-Cell and Cell-Matrix Interactions and Breast Cancer Bone Metastasis Modeling
Coggin, C., Chen, W., Burg, T. C., Dréau, D., & Burg, K. J. L. (2026) · Regenerative Engineering and Translational Medicine · doi:10.1007/s40883-026-00613-2
Boncristiani, C., Baldassarre, F., Eddahaoui, K., Ciccarella, G., & Vergaro, V. (2026) · Materials · doi:10.3390/ma19173729
Zheng, N., Tang, Z., Yang, G., Deng, L., Zhao, Q., Zhao, Y., Zhang, J., Wang, X., Chai, Y., Chen, Y., et al. (2026) · Bioactive Materials · doi:10.1016/j.bioactmat.2026.09.004
Wu, Y., Govindaraju, D. T., Lakshmikanthacharya, G. M., Hsu, T.-Y., Lin, C.-F., Sharma, S., & Anilkumar, T. S. (2026) · Pharmaceutics · doi:10.3390/pharmaceutics18101227
Tian, H., Li, G., Lian, Y., Liu, H., Fang, Y., Tong, Y., He, Z., Bian, Y., & Wei, G. (2026) · Frontiers in Pharmacology · doi:10.3389/fphar.2026.1926341
Engineering a Functional Angiogenesis-Enabled and Ovarian Cell-Specific Tumor Microenvironment Chip
Oktem, E., Ma, S.-Y., Chang, A. K.-W., Hsu, C.-H., & Jain, A. (2026) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-026-00951-9
Lin, H., Wang, K., Chen, H., Tan, G., Zhu, J., Liu, H., & Yang, J. (2026) · Critical Reviews in Oncology/Hematology · doi:10.1016/j.critrevonc.2026.105659
Rong, Y. A. N., Yinuo, H. E., ZHANG, Y., Xiaxia, W. A. N. G., Meng, Y. U., Jiuming, H., Huarong, X. U., & Yongjian, A. I. (2026) · Analysis and Testing Technology and Instruments · doi:10.3724/j.1006-3757.2026.05.002
Sharko, V., Olaizola‐Rodrigo, C., Bayona, C., Ranđelović, T., Monge, R., Solanas, E., Oliván, S., & Ochoa, I. (2026) · Advanced Healthcare Materials · doi:10.1002/adhm.71910
Jeelani, P. G. (2026) · Next Nanotechnology · doi:10.1016/j.nxnano.2026.100849
Metadata
- Authors
- Nashimoto, Y., Okada, R., Hanada, S., Arima, Y., Nishiyama, K., Miura, T., & Yokokawa, R.
- Affiliations
- Tohoku University (JP)
- Kyoto University (JP)
- International Research Center for Medical Sciences, Kumamoto University (JP)
- Kyushu University (JP)
- Corresponding author
- Yokokawa, Ryuji
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2020-01
- Volume
- 229
- Pages
- 119547
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2981018318
- Cited by
- 332
- References
- 44
- Citations by year
- 2026: 36 · 2025: 46 · 2024: 66 · 2023: 59 · 2022: 58 · 2021: 39 · 2020: 28
- FWCI
- 10.97
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biomaterials (acceptedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Nakatomi Foundation
- Japan Science and Technology Agency
- Japan Agency for Medical Research and Development
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Physical Sciences › Mathematics › Modeling and Simulation › Mathematical Biology Tumor Growth
- Keywords
- tumor-on-a-chip, tumor spheroids, perfusion, tumor vasculature, fibroblasts, tumor microenvironment, tumor cell proliferation, cell death, anticancer drug delivery, drug screening, blood flow
- MeSH terms
- Pharmaceutical Preparations, Humans, Neoplasms, Perfusion, Lab-On-A-Chip Devices, Tumor Microenvironment
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
