The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Efficient myotube formation in 3D bioprinted tissue construct by biochemical and topographical cues
Open accessAbstract
Biochemical and biophysical cues directly affect cell morphology, adhesion, proliferation, and phenotype, as well as differentiation; thus, they have been commonly utilized for designing and developing biomaterial systems for tissue engineering applications. To bioengineer skeletal muscle tissues, the efficient and stable formation of aligned fibrous multinucleated myotubes is essential. To achieve this goal, we employed a decellularized extracellular matrix (dECM) as a biochemical component and a modified three-dimensional (3D) cell-printing process to produce an in situ uniaxially aligned/micro-topographical structure. The dECM was derived from the decellularization of porcine skeletal muscles and chemically modified by methacrylate process to enhance mechanical stability. By using this ECM-based material and the 3D printing capability, we were able to produce a cell-laden dECM-based structure with unique topographical cues. The myoblasts (C2C12 cell line) laden in the printed structure were aligned and differentiated with a high degree of myotube formation, owing to the synergistic effect of the skeletal muscle-specific biochemical and topographical cues. In particular, the increase of the gene-expression levels of the dECM structure with topographical cues was approximately 1.5-1.8-fold compared with those of a gelatin methacrylate (GelMA)-based structure with the same topographical cues and a dECM-based structure without topographical cues. According to these in vitro cellular responses, the 3D printed dECM-based structures with topographical cues have the potential for bioengineering functional skeletal muscle tissues, and this strategy can be extended for many musculoskeletal tissues, such as tendons and ligaments and utilized for developing in vitro tissue-on-a-chip models in drug screening and development.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“This process ensures myoblast alignment and differentiation, crucial for myotube formation, by employing UV light for crosslinking and removing PVA post‐printing.[ 158 ]”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [158]
The cited source says what the claim attributes to it.
In the citing paper
“This process ensures myoblast alignment and differentiation, crucial for myotube formation, by employing UV light for crosslinking and removing PVA post‐printing.[ 158 ]”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [158]
The cited source says what the claim attributes to it.
In the citing paper
“Similarly, methacrylated dECM from porcine skeletal muscle, when combined with sacrificial fibrillated PVA, has been bioprinted into aligned skeletal muscle structures.”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [158]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
3D bioprinting of tissues and organs
Murphy, S. V., & Atala, A. (2014) · Nature Biotechnology · doi:10.1038/nbt.2958 · Cited by 7,034
Self-citationAn overview of tissue and whole organ decellularization processes
Crapo, P. M., Gilbert, T. W., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.057 · Cited by 3,591
A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
Kang, H.-W., Lee, S. J., Ko, I. K., Kengla, C., Yoo, J. J., & Atala, A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3413 · Cited by 2,622
Self-citationDetermination of free amino groups in proteins by trinitrobenzenesulfonic acid
Habeeb, A. F. S. A. (1966) · Analytical Biochemistry · doi:10.1016/0003-2697(66)90275-2 · Cited by 2,040
Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink
Pati, F., Jang, J., Ha, D.-H., Won Kim, S., Rhie, J.-W., Shim, J.-H., Kim, D.-H., & Cho, D.-W. (2014) · Nature Communications · doi:10.1038/ncomms4935 · Cited by 1,904
Extracellular matrix as a biological scaffold material: Structure and function
BADYLAK, S., FREYTES, D., & GILBERT, T. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2008.09.013 · Cited by 1,671
Structural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels
Van Den Bulcke, A. I., Bogdanov, B., De Rooze, N., Schacht, E. H., Cornelissen, M., & Berghmans, H. (2000) · Biomacromolecules · doi:10.1021/bm990017d · Cited by 1,536
Reprint of: Extracellular matrix as a biological scaffold material: Structure and function
Badylak, S. F., Freytes, D. O., & Gilbert, T. W. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.07.016 · Cited by 1,367
Printing and Prototyping of Tissues and Scaffolds
Derby, B. (2012) · Science · doi:10.1126/science.1226340 · Cited by 1,126
Leong, K. F., Cheah, C. M., & Chua, C. K. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00030-9 · Cited by 1,090
Cited by
Sorted by citations
Kim, B. S., Das, S., Jang, J., & Cho, D.-W. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.9b00808 · Cited by 465
Extracellular matrix: an important regulator of cell functions and skeletal muscle development
Zhang, W., Liu, Y., & Zhang, H. (2021) · Cell & Bioscience · doi:10.1186/s13578-021-00579-4 · Cited by 289
Golebiowska, A. A., Intravaia, J. T., Sathe, V. M., Kumbar, S. G., & Nukavarapu, S. P. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.09.017 · Cited by 236
Designing Decellularized Extracellular Matrix‐Based Bioinks for 3D Bioprinting
Abaci, A., & Güvendiren, M. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.202000734 · Cited by 184
3D bioprinting: current status and trends—a guide to the literature and industrial practice
Santoni, S., Gugliandolo, S. G., Sponchioni, M., Moscatelli, D., & Colosimo, B. M. (2021) · Bio-Design and Manufacturing · doi:10.1007/s42242-021-00165-0 · Cited by 172
Metadata
- Authors
- Kim, W., Lee, H., Lee, J., Atala, A., Yoo, J. J., Lee, S. J., & Kim, G. H.
- Affiliations
- Wake Forest University Health Sciences (US)
- Corresponding author
- Lee, Sang Jin
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2020-02
- Volume
- 230
- Pages
- 119632
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC7141931
- OpenAlex
- W2991322883
- Cited by
- 185
- References
- 55
- Citations by year
- 2026: 13 · 2025: 21 · 2024: 32 · 2023: 33 · 2022: 33 · 2021: 36 · 2020: 16
- FWCI
- 20.55
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Research Foundation of Korea
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- myotube formation, 3D bioprinting, decellularized extracellular matrix, skeletal muscle tissue engineering, uniaxial alignment, microtopography, myogenesis, C2C12 myoblasts, porcine skeletal muscle, gelatin methacryloyl, gene expression
- MeSH terms
- Animals, Cues, Extracellular Matrix, Swine, Muscle Fibers, Skeletal, Tissue Engineering, Bioprinting, Printing, Three-Dimensional
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
