Structural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels
Abstract
Dynamic shear oscillation measurements at small strain were used to characterize the viscoelastic properties and related differences in the molecular structure of hydrogels based on gelatin methacrylamide. Gelatin was derivatized with methacrylamide side groups and was subsequently cross-linked by radical polymerization via photoinitiation. The light treatment of methacrylamide gelatin solutions resulted in the production of hydrogel films with high storage modulus (G'). Mechanical spectra and thermal scanning rheology of the obtained hydrogels are described. The temperature scan of the network below and above melting point of gelatin allowed us to identify the respective contributions of chemical and physical cross-linkage to the hydrogel elastic modulus. The results indicate that the rheological properties of the gelatin-based hydrogels can be controlled by the degree of substitution, polymer concentration, initiator concentration, and UV irradiation conditions.
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3D scaffolds and associated cell-encapsulation techniques provide valuable tools for deciphering how ECM affects fate of cells.
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Due to chemical functionalization that disrupts native intermolecular interactions, the protein matrix requires less thermal energy to undergo conformational rearrangements, leading to a downward shift of DSC transition temperatures.
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Cites
Determination of free amino groups in proteins by trinitrobenzenesulfonic acid
Habeeb, A. F. S. A. (1966) · Analytical Biochemistry · doi:10.1016/0003-2697(66)90275-2 · Cited by 2,040 · see citing works
Hydrogels in Medicine and Pharmacy
Peppas, N. A. (1987) · Cited by 1,636 · see citing works
The science and technology of gelatin
Veis, A. (1978) · Journal of Colloid and Interface Science · doi:10.1016/0021-9797(78)90323-5 · Cited by 706 · see citing works
Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices
Jayakrishnan, A., & Jameela, S. R. (1996) · Biomaterials · doi:10.1016/0142-9612(96)82721-9 · Cited by 500 · see citing works
Biological effects of residual glutaraldehyde in glutaraldehyde‐tanned collagen biomaterials
Speer, D. P., Chvapil, M. S., Eskelson, C. D., & Ulreich, J. B. (1980) · Journal of Biomedical Materials Research · doi:10.1002/jbm.820140607 · Cited by 473 · see citing works
Thermoreversible gelation of polymers and biopolymers
Ross-Murphy, S. B. (1993) · Polymer · doi:10.1016/0032-3861(93)90142-w · Cited by 467 · see citing works
The macromolecular chemistry of gelatin
Mandl, I. (1965) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(65)90257-2 · Cited by 311 · see citing works
Concentration dependence of the critical viscoelastic properties of gelatin at the gel point
Michon, C., Cuvelier, G., & Launay, B. (1993) · Rheologica Acta · doi:10.1007/bf00396681 · Cited by 224 · see citing works
Evaluation of different chemical methods for cros-linking collagen gel, films and sponges
Rault, I., Frei, V., Herbage, D., Abdul‐Malak, N., & Huc, A. (1996) · Journal of Materials Science Materials in Medicine · doi:10.1007/bf00119733 · Cited by 219 · see citing works
Immobilization of proteins in microspheres of biodegradable polyacryldextran
Edman, P., Ekman, B., & Sjöholm, I. (1980) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.2600690725 · Cited by 156 · see citing works
Use of the acyl azide method for cross‐linking collagen‐rich tissues such as pericardium
Petite, H., Rault, I., Huc, A., Menasché, P., & Herbage, D. (1990) · Journal of Biomedical Materials Research · doi:10.1002/jbm.820240205 · Cited by 147 · see citing works
Influence of thermal treatments on the structure and stability of gelatin gels
Djabourov, M., & Papon, P. (1983) · Polymer · doi:10.1016/0032-3861(83)90101-5 · Cited by 144 · see citing works
Homogeneous grafting reaction of vinyl pyrrolidone onto chitosan
Yazdani‐Pedram, M., & RETUERT, J. (1997) · Journal of Applied Polymer Science · doi:10.1002/(sici)1097-4628(19970307)63:10 · Cited by 84 · see citing works
Hydrogels prepared by crosslinking of gelatin with dextran dialdehyde
Schacht, E. H., Bogdanov, B. G., Bulcke, A. V. D., et al. (1997) · Reactive and Functional Polymers · doi:10.1016/s1381-5148(97)00047-3 · Cited by 84 · see citing works
Self-citationGelation in physically associating biopolymer systems
Carnali, J. O. (1992) · Rheologica Acta · doi:10.1007/bf00701120 · Cited by 52 · see citing works
Some aspects of the crosslinking of gelatin by dextran dialdehydes
Schacht, E. H., Nobels, M., Vansteenkiste, S. O., et al. (1993) · Polymer Gels and Networks · doi:10.1016/0966-7822(93)90001-x · Cited by 37 · see citing works
Self-citationThe growth of chondrocytes using Gelfoam� as a biodegradable scaffold
Stanton, S. K., Salih, V. M., Bentley, G., et al. (1995) · Journal of Materials Science Materials in Medicine · doi:10.1007/bf00134310 · Cited by 31 · see citing works
(1997) · Advances in polymer science · doi:10.1007/bfb0008710 · Cited by 15 · see citing works
Thermal and rheological properties of gelatin-dextran hydrogels
Bogdanov, B. G., Schacht, E. H., & Bulcke, A. V. D. (1997) · Journal of thermal analysis · doi:10.1007/bf01996769 · Cited by 13 · see citing works
Self-citationDimethyl sulfoxide as an anticalcification agent for glutaraldehyde-fixed biological tissue
Khor, E., Wee, A. T. S., Loke, W. K., et al. (1996) · Journal of Materials Science Materials in Medicine · doi:10.1007/bf00123408 · Cited by 3 · see citing works
Anthracycline antitumour agents. A review of physicochemical, analytical and stability properties
Bouma, J., Beijnen, J. H., Bult, A., et al. (1986) · Pharmacy World & Science · doi:10.1007/bf01959776 · Cited by 3 · see citing works
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Yue, K., Trujillo-de Santiago, G., Alvarez, M. M., Tamayol, A., Annabi, N., & Khademhosseini, A. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.045 · Cited by 3,146 · see citing works
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BackgroundInfluentialCell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377 · see citing works
3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs
Kolesky, D. B., Truby, R. L., Gladman, A. S., Busbee, T. A., Homan, K. A., & Lewis, J. A. (2014) · Advanced Materials · doi:10.1002/adma.201305506 · Cited by 2,102 · see citing works
Modeling physiological events in 2D vs. 3D cell culture
Duval, K., Grover, H., Han, L.-H., Mou, Y., Pegoraro, A. F., Fredberg, J., & Chen, Z. (2017) · Physiology · doi:10.1152/physiol.00036.2016 · Cited by 1,849 · see citing works
Billiet, T., Gevaert, E., De Schryver, T., Cornelissen, M., & Dubruel, P. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.09.078 · Cited by 1,015 · see citing works
Self-citationLoessner, D., Meinert, C., Kaemmerer, E., Martine, L. C., Yue, K., Levett, P. A., Klein, T. J., Melchels, F. P. W., Khademhosseini, A., & Hutmacher, D. W. (2016) · Nature Protocols · doi:10.1038/nprot.2016.037 · Cited by 868 · see citing works
Gauvin, R., Chen, Y.-C., Lee, J. W., Soman, P., Zorlutuna, P., Nichol, J. W., Bae, H., Chen, S., & Khademhosseini, A. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.01.048 · Cited by 641 · see citing works
“In the present study, a custom designed projection stereolithography (PSL) system was used to control the 3D microarchitecture of collagen-based gelatin methacrylate (GelMA) hydrogels [21,43].”
MethodZhao, X., Liu, S., Yildirimer, L., Zhao, H., Ding, R., Wang, H., Cui, W., & Weitz, D. (2016) · Advanced Functional Materials · doi:10.1002/adfm.201504943 · Cited by 426 · see citing works
Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications
Choi, J. R., Yong, K. W., Choi, J. Y., & Cowie, A. C. (2019) · BioTechniques · doi:10.2144/btn-2018-0083 · Cited by 403 · see citing works
3D bioprinted silk fibroin hydrogels for tissue engineering
Kim, S. H., Hong, H., Ajiteru, O., Sultan, M. T., Lee, Y. J., Lee, J. S., Lee, O. J., Lee, H., Park, H. S., Choi, K. Y., et al. (2021) · Nature Protocols · doi:10.1038/s41596-021-00622-1 · Cited by 294 · see citing works
Liu, Y., & Chan-Park, M. B. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2009.10.040 · Cited by 285 · see citing works
Gelatin methacryloyl hydrogel, from standardization, performance, to biomedical application
He, J., Sun, Y., Gao, Q., He, C., Yao, K., Wang, T., Xie, M., Yu, K., Nie, J., Chen, Y., et al. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300395 · Cited by 275 · see citing works
Gelatine Methacrylamide-Based Hydrogels: An Alternative Three-Dimensional Cancer Cell Culture System
Kaemmerer, E., Melchels, F. P. W., Holzapfel, B. M., Meckel, T., Hutmacher, D. W., & Loessner, D. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.02.035 · Cited by 219 · see citing works
Efficient myotube formation in 3D bioprinted tissue construct by biochemical and topographical cues
Kim, W., Lee, H., Lee, J., Atala, A., Yoo, J. J., Lee, S. J., & Kim, G. H. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2019.119632 · Cited by 185 · see citing works
A multilayered microfluidic blood vessel-like structure
Hasan, A., Paul, A., Memic, A., & Khademhosseini, A. (2015) · Biomedical Microdevices · doi:10.1007/s10544-015-9993-2 · Cited by 139 · see citing works
Wang, T., Lai, J. H., Han, L.-H., Tong, X., & Yang, F. (2014) · Tissue Engineering Part A · doi:10.1089/ten.tea.2013.0531 · Cited by 70 · see citing works
Lee, B. H., Shirahama, H., Kim, M. H., Lee, J. H., Cho, N.-J., & Tan, L. P. (2017) · NPG Asia Materials · doi:10.1038/am.2017.126 · Cited by 52 · see citing works
Ghazali, H. S., Askari, E., Seyfoori, A., & Naghib, S. M. (2023) · Scientific Reports · doi:10.1038/s41598-023-35865-3 · Cited by 40 · see citing works
Variation in hydrogel formation and network structure for telo-, atelo- and methacrylated collagens
Maher, M. K., White, J. F., Glattauer, V., Yue, Z., Hughes, T. C., Ramshaw, J. A. M., & Wallace, G. G. (2022) · Polymers · doi:10.3390/polym14091775 · Cited by 17 · see citing works
Antunes, B. S. L., Pedrini, F., Komatsu, D., Asami, J., Dicko, C., & Duek, E. A. R. (2026) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2026.154129
- Authors
- Van Den Bulcke, A. I., Bogdanov, B., De Rooze, N., Schacht, E. H., Cornelissen, M., & Berghmans, H.
- Affiliations
- Department of Organic Chemistry, Polymer Materials Research Group, Institute of Biomedical Technologies (IBITECH), University of Ghent, Krijgslaan 281, S4-bis, B-9000 Ghent, Belgium; Department of Anatomy, Embryology and Histology, Faculty of Medicines, University of Ghent, Godshuizenlaan 4, B-9000 Ghent, Belgium; and Laboratory for Polymer Research, Department Chemistry, University of Leuven, Celestijnenlaan 200F, B-3001 Heverlee, Belgium
- Ghent University (BE)
- Corresponding author
- Schacht, Etienne H.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-02-10
- Volume
- 1
- Issue
- 1
- Pages
- 31-38
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2077443558
- Cited by
- 1,536
- References
- 21
- Citations by year
- 2026: 110 · 2025: 149 · 2024: 173 · 2023: 179 · 2022: 145 · 2021: 125 · 2020: 134 · 2019: 91 · 2018: 80 · 2017: 61 · 2016: 69 · 2015: 51 · 2014: 34 · 2013: 33 · 2012: 21
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomacromolecules (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Funders
- Fonds Wetenschappelijk Onderzoek
- Vlaamse regering
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Keywords
- gelatin hydrogel, viscoelasticity, photopolymerization, crosslinking, physical crosslinking, storage modulus, degree of substitution, UV irradiation, hydrogel films
- MeSH terms
- Acrylamides, Chemistry, Physical, Cross-Linking Reagents, Gelatin, Rheology, Spectrophotometry, Ultraviolet, Thermodynamics, Hydrogels, Chemical Phenomena
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