Chlorophyll production from Spirulina platensis: cultivation with urea addition by fed-batch process
Abstract
The cyanobacterium Spirulina platensis is an attractive alternative source of the pigment chlorophyll, which is used as a natural color in food, cosmetic, and pharmaceutical products. In this work, the influence of the light intensity and urea supplementation as a nitrogen source using fed-batch cultivation for S. platensis growth and chlorophyll content was examined. Cultivations were carried out in 5 l open tanks, at 30+/-1 degrees C. Response surface methodology was utilized for analysis of the results, and models were obtained for biomass productivity, nitrogen-cell conversion factor and chlorophyll productivity. The best cellular growth was observed with 500 mg/l of urea at a light intensity of 5600 lx, whereas the highest concentration of chlorophyll in the biomass was observed with 500 mg/l of urea at a light intensity of 1400 lx. Overall, the best chlorophyll productivity was observed with 500 mg/l of urea at a light intensity of 3500 lx, providing the optimal balance between the cellular growth and the biomass chlorophyll content.
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Aphanizomenon flos-aquae, Chlorella sp., Dunaliella salina, Dunaliella tertiolecta and Spirulina plantensis are some of the microalgae species widely used as a human food source because they are rich in protein content and have high nutritive value.
“Aphanizomenon flos-aquae, Chlorella sp., Dunaliella salina (D. salina), Dunaliella tertiolecta (D. tertiolecta) and Spirulina plantensis (S. plantensis) are some of the microalgae species widely used as a human food source because they are rich in protein content and have high nutritive value (Soletto et al., 2005, Rangel-Yagui et al., 2004).”
From Microalgae Metabolites: A Rich Source for Food and Medicine (2019) · cites this paper as Rangel-Yagui et al., 2004
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- Authors
- Rangel-Yagui, C. D. O., Danesi, E. D. G., de Carvalho, J. C. M., & Sato, S.
- Affiliations
- Universidade de São Paulo (BR)
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2004-04
- Volume
- 92
- Issue
- 2
- Pages
- 133-141
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W1977884612
- Cited by
- 187
- References
- 33
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 13 · 2023: 7 · 2022: 10 · 2021: 15 · 2020: 14 · 2019: 5 · 2018: 6 · 2017: 5 · 2016: 12 · 2015: 7 · 2014: 10 · 2013: 12 · 2012: 13
- FWCI
- 2.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
- Keywords
- Spirulina platensis, chlorophyll biosynthesis, urea supplementation, nitrogen source, fed-batch fermentation, light intensity, cyanobacteria, biomass productivity, natural colorants
- MeSH terms
- Cyanobacteria, Analysis of Variance, Chlorophyll, Light, Models, Biological, Nitrogen, Time Factors, Urea, Biomass, Bioreactors
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