Cited by 187Algal biology and biofuel productionAquatic Ecosystems and Phytoplankton DynamicsMicrobial Metabolism and Applications

Chlorophyll production from Spirulina platensis: cultivation with urea addition by fed-batch process

Rangel-Yagui, Carlota de Oliveira, Danesi, Eliane Dalva Godoy, de Carvalho, João Carlos Monteiro, Sato, Sunao·2004·Bioresource Technology

Abstract

The cyanobacterium Spirulina platensis is an attractive alternative source of the pigment chlorophyll, which is used as a natural color in food, cosmetic, and pharmaceutical products. In this work, the influence of the light intensity and urea supplementation as a nitrogen source using fed-batch cultivation for S. platensis growth and chlorophyll content was examined. Cultivations were carried out in 5 l open tanks, at 30+/-1 degrees C. Response surface methodology was utilized for analysis of the results, and models were obtained for biomass productivity, nitrogen-cell conversion factor and chlorophyll productivity. The best cellular growth was observed with 500 mg/l of urea at a light intensity of 5600 lx, whereas the highest concentration of chlorophyll in the biomass was observed with 500 mg/l of urea at a light intensity of 1400 lx. Overall, the best chlorophyll productivity was observed with 500 mg/l of urea at a light intensity of 3500 lx, providing the optimal balance between the cellular growth and the biomass chlorophyll content.

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Crossref · 10.1016/j.biortech.2003.09.002

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  • Aphanizomenon flos-aquae, Chlorella sp., Dunaliella salina, Dunaliella tertiolecta and Spirulina plantensis are some of the microalgae species widely used as a human food source because they are rich in protein content and have high nutritive value.

    “Aphanizomenon flos-aquae, Chlorella sp., Dunaliella salina (D. salina), Dunaliella tertiolecta (D. tertiolecta) and Spirulina plantensis (S. plantensis) are some of the microalgae species widely used as a human food source because they are rich in protein content and have high nutritive value (Soletto et al., 2005, Rangel-Yagui et al., 2004).”

    From Microalgae Metabolites: A Rich Source for Food and Medicine (2019) · cites this paper as Rangel-Yagui et al., 2004

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Authors
Rangel-Yagui, C. D. O., Danesi, E. D. G., de Carvalho, J. C. M., & Sato, S.
Affiliations
Universidade de São Paulo (BR)
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2004-04
Volume
92
Issue
2
Pages
133-141
ISSN
0960-8524 (ISSN-L)
OpenAlex
W1977884612
Cited by
187
References
33
Citations by year
2026: 6 · 2025: 7 · 2024: 13 · 2023: 7 · 2022: 10 · 2021: 15 · 2020: 14 · 2019: 5 · 2018: 6 · 2017: 5 · 2016: 12 · 2015: 7 · 2014: 10 · 2013: 12 · 2012: 13
FWCI
2.28
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
Keywords
Spirulina platensis, chlorophyll biosynthesis, urea supplementation, nitrogen source, fed-batch fermentation, light intensity, cyanobacteria, biomass productivity, natural colorants
MeSH terms
Cyanobacteria, Analysis of Variance, Chlorophyll, Light, Models, Biological, Nitrogen, Time Factors, Urea, Biomass, Bioreactors

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