Enhancement of Neuroprotective Effects of Spirulina platensis Extract from a High-Pressure Homogenization Process
Open accessAbstract
This study is the first to show that a simple high-pressure homogenization process (HP) could extract very heat-sensitive chlorophyll from a marine alga, Spirulina platensis. Up to 9.85 mg/g was produced at an optimal input pressure of 650 bar with a shear stress of approximately 20,000 (1/s) compared to 5.27 mg/g from conventional 70% ethanol extraction (EE) at 40 °C for 24 h. However, a higher input pressure did not increase the extraction yield and in fact decreased it, possibly due to the destruction of chlorophyll a at a high shear stress of approximately 50,000 (1/s) under 1370 bar of input pressure. Chlorophyll a and other components of the extract had additive effects, showing higher antioxidant activities of ca. 56% of DPPH radical scavenging activities than 10 μg/mL chlorophyll a alone, which corresponded to the same chlorophyll a content that existed in the extract. It was also confirmed that the high antioxidant activities of the HP extract reduced the production of reactive oxygen species (ROS) from glutamate-induced HT22 cells, which resulted in increased neuroprotective activity compared to EE and even chlorophyll a alone, in addition to effectively reducing the release of Ca2+ from nerve cells.
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C-PC supports neuro- and hepatoprotective pathways.
“supports neuro-and hepatoprotective pathways [59][60][61],”
From Valorization of Microalgae as Multifunctional Protein Sources in Plant-Based Systems: Structural, Functional, and Processing Challenges (2026) · cites this paper as [61]
The Introduction identifies antioxidant and anti-inflammatory effects of C-PC, and the Discussion links antioxidant activity to neuroprotection by the Spirulina extract. The paper does not establish that C-PC itself provides the observed neuroprotection, and our copy does not address hepatoprotective pathways. (LLM, conf=0.76)
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- Authors
- Choi, W. Y., & Lee, H. Y.
- Affiliations
- Kangwon National University (KR)
- Seowon University (KR)
- Corresponding author
- Lee, Hyeon Yong
- ORCID
- Lee, Hyeon Yong
- Venue
- Applied Sciences
- Publisher
- MDPI AG
- Published
- 2018-04-19
- Volume
- 8
- Issue
- 4
- Pages
- 634
- ISSN
- 2076-3417 (ISSN-L)
- OpenAlex
- W2801330941
- Cited by
- 15
- References
- 28
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Applied Sciences (publishedVersion, cc-by)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- Spirulina platensis, high-pressure homogenization, neuroprotection, antioxidant activity, reactive oxygen species, HT22 cells, glutamate excitotoxicity, calcium release, marine algae extracts
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