Cited by 156Biofuel production and bioconversionCatalysis for Biomass ConversionAnaerobic Digestion and Biogas Production

Enhanced enzymatic hydrolysis of sugarcane bagasse by N-methylmorpholine-N-oxide pretreatment

Kuo, Chia-Hung, Lee, Cheng-Kang·2009·Bioresource Technology

Abstract

The cellulose dissolution solvent used in Lyocell process for cellulose fiber preparation, N-methylmorpholine-N-oxide (NMMO) monohydrate, was demonstrated to be an effective agent for sugarcane bagasse pretreatment. Bagasse of 20wt% was readily dissolved in NMMO monohydrate at 130 degrees C within 1h. After dissolution, bagasse could be regenerated by rapid precipitation with water as a porous and amorphous mixture of its original components. The regenerated bagasse exhibited a significant enhancement on enzymatic hydrolysis kinetic. Not only the reducing sugars releasing rate but also hydrolysis yield was enhanced at least twofold as compared with that of untreated bagasse. The cellulose fraction of regenerated bagasse was nearly hydrolyzed to glucose after 72h hydrolysis with Cellulase AP3. The recycled NMMO demonstrated the same performance as the fresh one on bagasse pretreatment for hydrolysis enhancement. The regenerated bagasse was directly used in simultaneous saccharification and fermentation (SSF) for ethanol production by Zymomonas mobilis. No negative effect on ethanol fermentation was observed and ethanol yield approximately 0.15 g ethanol/g baggasse was achieved.

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Crossref · 10.1016/j.biortech.2008.07.001

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23 of 23 references verified0 citation errors · 0 not checkable

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  • The Viscose process uses toxic and highly flammable CS2 and produces large amounts of waste products, which has fuelled the search for a more benign replacement.

    “This process uses toxic and highly flammable CS 2 and produces large amounts of waste products, which has fuelled the search for a more benign replacement.”

    From Deconstruction of lignocellulosic biomass with ionic liquids (2013) · cites this paper as [65]

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Authors
Kuo, C.-H., & Lee, C.-K.
Affiliations
National Taiwan University of Science and Technology (TW)
Corresponding author
Lee, Cheng-Kang
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2009-01
Volume
100
Issue
2
Pages
866-871
ISSN
0960-8524 (ISSN-L)
OpenAlex
W2054175123
Cited by
156
References
23
Citations by year
2025: 3 · 2024: 2 · 2023: 9 · 2022: 7 · 2021: 6 · 2020: 3 · 2019: 4 · 2018: 11 · 2017: 8 · 2016: 15 · 2015: 9 · 2014: 9 · 2013: 17 · 2012: 14
FWCI
8.63
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Physical Sciences › Engineering › Biomedical Engineering › Catalysis for Biomass Conversion
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Keywords
sugarcane bagasse, N-methylmorpholine N-oxide, enzymatic hydrolysis, cellulose dissolution, reducing sugar release, hydrolysis yield, simultaneous saccharification and fermentation, ethanol production, Zymomonas mobilis
MeSH terms
Cellulose, Cyclic N-Oxides, Enzyme Activation, Hydrolysis, Morpholines, Substrate Specificity, Saccharum

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