Fractionating recalcitrant lignocellulose at modest reaction conditions
Abstract
Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre‐treatment technologies suffer from low sugar yields, and/or severe reaction conditions, and/or high cellulase use, narrow substrate applicability, and high capital investment, etc. A new lignocellulose pre‐treatment featuring modest reaction conditions (50°C and atmospheric pressure) was demonstrated to fractionate lignocellulose to amorphous cellulose, hemicellulose, lignin, and acetic acid by using a non‐volatile cellulose solvent (concentrated phosphoric acid), a highly volatile organic solvent (acetone), and water. The highest sugar yields after enzymatic hydrolysis were attributed to no sugar degradation during the fractionation and the highest enzymatic cellulose digestibility (∼97% in 24 h) during the hydrolysis step at the enzyme loading of 15 filter paper units of cellulase and 60 IU of beta‐glucosidase per gram of glucan. Isolation of high‐value lignocellulose components (lignin, acetic acid, and hemicellulose) would greatly increase potential revenues of a lignocellulose biorefinery. Biotechnol. Bioeng. 2007;97: 214–223. © 2007 Wiley Periodicals, Inc.
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The Lyocell process is a true dissolution process using NMO and an improvement in terms of health and environmental impact.
“The Lyocell process is a true dissolution process using NMO and an improvement in terms of health and environmental impact.66”
From Deconstruction of lignocellulosic biomass with ionic liquids (2013) · cites this paper as [66]
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- Authors
- Zhang, Y. P., Ding, S., Mielenz, J. R., Cui, J., Elander, R. T., Laser, M., Himmel, M. E., McMillan, J. R., & Lynd, L. R.
- Affiliations
- Virginia Tech (US)
- National Laboratory of the Rockies (US)
- Oak Ridge National Laboratory (US)
- Dartmouth College (US)
- Corresponding author
- Zhang, Yi‐Heng Percival
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2007-02-22
- Volume
- 97
- Issue
- 2
- Pages
- 214-223
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2132982284
- Cited by
- 589
- References
- 56
- Citations by year
- 2026: 9 · 2025: 7 · 2024: 15 · 2023: 17 · 2022: 21 · 2021: 32 · 2020: 19 · 2019: 26 · 2018: 36 · 2017: 41 · 2016: 30 · 2015: 28 · 2014: 40 · 2013: 59 · 2012: 51
- FWCI
- 24.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Engineering › Biomedical Engineering › Catalysis for Biomass Conversion
- Physical Sciences › Engineering › Biomedical Engineering › Lignin and Wood Chemistry
- Keywords
- lignocellulose fractionation, lignocellulosic biomass pretreatment, phosphoric acid pretreatment, acetone, enzymatic hydrolysis, cellulase, β-glucosidase, sugar yield, lignin, hemicellulose, acetic acid, lignocellulosic biorefinery, low-temperature pretreatment
- MeSH terms
- Cellulose, Chemical Fractionation, Hydrolysis, Lignin, Solvents
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