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Lipid analysis in Haematococcus pluvialis to assess its potential use as a biodiesel feedstock
Open accessAbstract
The lipid content and composition of Haematococcuspluvialis exposed to stress conditions were analyzed to assess the potential of this microalga as a biodiesel feedstock. The total lipid content of control cells was 15.61% dw, whereas that of cells exposed to continuous high light intensity with nitrogen-sufficient medium (A-stress condition) or under continuous high light intensity with nitrogen-deprivation medium (B-stress condition) was 34.85% dw and 32.99% dw, respectively. The fatty acid profile was similar under all conditions and indicated that the main components were palmitic, stearic, oleic, linoleic, linolenic and linolelaidic acids. The neutral lipid fraction increased about 2-fold under both stress conditions. The percentage of saturated fatty acids in the neutral lipid fraction was 30.36% and 29.62% in cultures grown under A-stress and B-stress, respectively, and 27.81% under control conditions. The monounsaturated fatty acid content was not significantly different in control and A-stress cultures (20.07% and 19.91%, respectively), but was 18.96% under B-stress. The content of polyunsaturated fatty acids was 47.23% under B-stress and 43.15% under A-stress. Growth-rate was higher under A-stress compared to B-stress. This is the first study of H.pluvialis that provides a detailed characterization of its lipid content in relation to bioenergy. The results indicate the potential of this microalga as a biodiesel feedstock; however, culture conditions still have to be improved in order to achieve an adequate energy balance in mass culture.
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“Green algae (Chlorophyceae) have the largest group of oleaginous microalgae, with high lipid abundance, and are good potential biodiesel feedstocks [37, 44, 45].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [44]
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Cites
Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524
Myers, R. H., & Montgomery, D. C. (1996) · IIE Transactions · doi:10.1080/15458830.1996.11770760 · Cited by 6,141
Microalgae for biodiesel production and other applications: A review
Mata, T. M., Martins, A. A., & Caetano, N. S. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2009.07.020 · Cited by 5,685
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745
Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G., & Tredici, M. R. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22033 · Cited by 2,879
Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters
Knothe, G. (2004) · Fuel Processing Technology · doi:10.1016/j.fuproc.2004.11.002 · Cited by 2,386
Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production
Schenk, P. M., Thomas‐Hall, S. R., Stephens, E., Marx, U. C., Mussgnug, J. H., Posten, C. H., Kruse, O., & Hankamer, B. D. (2008) · BioEnergy Research · doi:10.1007/s12155-008-9008-8 · Cited by 2,335
Sheehan, J., Dunahay, T., Benemann, J., & Roessler, P. (1998) · doi:10.2172/15003040 · Cited by 1,753
Official methods and recommended practices of the AOCS
Firestone, D. (2009) · Cited by 1,635
Handbook of phycological methods, culture methods and growth measurements
den Hartog, C. (1974) · Aquaculture · doi:10.1016/0044-8486(74)90057-x · Cited by 1,451
Lipid productivity as a key characteristic for choosing algal species for biodiesel production
Griffiths, M. J., & Harrison, S. T. L. (2009) · Journal of Applied Phycology · doi:10.1007/s10811-008-9392-7 · Cited by 1,421
Andersen, R. A., & Phycological Society of America (2005) · Choice Reviews Online · doi:10.5860/choice.43-0925 · Cited by 1,293
Microalgae as a raw material for biofuels production
Gouveia, L., & Oliveira, A. C. (2009) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-008-0495-6 · Cited by 1,284
Xu, H., Miao, X., & Wu, Q. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2006.05.002 · Cited by 1,141
Lipids and lipid metabolism in eukaryotic algae
Guschina, I. A., & Harwood, J. L. (2006) · Progress in Lipid Research · doi:10.1016/j.plipres.2006.01.001 · Cited by 1,110
Increase in Chlorella strains calorific values when grown in low nitrogen medium
Illman, A. M., Scragg, A. H., & Shales, S. W. (2000) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(00)00266-0 · Cited by 1,008
Handbook of microalgal culture: biotechnology and applied phycology.
RICHMOND, A. E. (2004) · Cited by 864
Handbook of Phycological methods - Culture methods and Growth measurements
Stein, J. R. (1973) · Cited by 852
Preparation and characterization of bio-diesels from various bio-oils
Lang, X. (2001) · Bioresource Technology · doi:10.1016/s0960-8524(01)00051-7 · Cited by 725
Biodiesel production—current state of the art and challenges
Vasudevan, P. T., & Briggs, M. (2008) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-008-0312-2 · Cited by 725
Botryococcus braunii : A Renewable Source of Hydrocarbons and Other Chemicals
Banerjee, A., Sharma, R., Yusuf Chisti, B., & Banerjee, U. C. (2002) · Critical Reviews in Biotechnology · doi:10.1080/07388550290789513 · Cited by 714
Plant triacylglycerols as feedstocks for the production of biofuels
Durrett, T. P., Benning, C., & Ohlrogge, J. B. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03442.x · Cited by 707
CO2 Mitigation and Renewable Oil from Photosynthetic Microbes: A New Appraisal
Huntley, M. E., & Redalje, D. G. (2007) · Mitigation and Adaptation Strategies for Global Change · doi:10.1007/s11027-006-7304-1 · Cited by 633
Carotenogenesis in the green alga Haematococcus pluvialis : Cellular physiology and stress response
Boussiba, S. (2000) · Physiologia Plantarum · doi:10.1034/j.1399-3054.2000.108002111.x · Cited by 591
Microalgae for aquaculture: Opportunities and constraints
Borowitzka, M. A. (1997) · Journal of Applied Phycology · doi:10.1023/a:1007921728300 · Cited by 478
Structure indices in FA chemistry. How relevant is the iodine value?
Knothe, G. (2002) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-002-0569-4 · Cited by 447
CHEMICAL PROFILE OF SELECTED SPECIES OF MICROALGAE WITH EMPHASIS ON LIPIDS1
Ben‐Amotz, A., Tornabene, T. G., & Thomas, W. H. (1985) · Journal of Phycology · doi:10.1111/j.0022-3646.1985.00072.x · Cited by 420
Roessler, P. G. (1990) · Journal of Phycology · doi:10.1111/j.0022-3646.1990.00393.x · Cited by 393
Olaizola, M. (2000) · Journal of Applied Phycology · doi:10.1023/a:1008159127672 · Cited by 359
Diesel fuel derived from vegetable oils, VI: Specifications and quality control of biodiesel
Mittelbach, M. (1996) · Bioresource Technology · doi:10.1016/0960-8524(95)00172-7 · Cited by 296
Belarbi, E. H., Molina, E., & Chisti, Y. (2000) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(99)00191-x · Cited by 281
Fidalgo, P., Cid, Á., Torres, E., Sukenik, A., & Herrero, C. (1998) · Aquaculture · doi:10.1016/s0044-8486(98)00278-6 · Cited by 273
Zhekisheva, M., Boussiba, S., Khozin‐Goldberg, I., Zarka, A., & Cohen, Z. (2002) · Journal of Phycology · doi:10.1046/j.1529-8817.2002.01107.x · Cited by 267
Rabbani, S., Beyer, P., Lintig, J. V., Hugueney, P., & Kleinig, H. (1998) · PLANT PHYSIOLOGY · doi:10.1104/pp.116.4.1239 · Cited by 263
Fluorescent measurement of microalgal neutral lipids
Elsey, D., Jameson, D., Raleigh, B., & Cooney, M. J. (2007) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2006.11.008 · Cited by 262
Boussiba, S., Bing, W., Yuan, J.-P., Zarka, A., & Chen, F. (1999) · Biotechnology Letters · doi:10.1023/a:1005507514694 · Cited by 247
Lipid composition of the red alga exposed to different levels of photon irradiance
Khotimchenko, S. V., & YAKOVLEVA, I. M. (2004) · Phytochemistry · doi:10.1016/j.phytochem.2004.10.024 · Cited by 245
Zittelli, G. C., Rodolfi, L., Biondi, N., & Tredici, M. R. (2006) · Aquaculture · doi:10.1016/j.aquaculture.2006.08.011 · Cited by 233
Borowitzka, M. A., Huisman, J. M., & Osborn, A. (1991) · Journal of Applied Phycology · doi:10.1007/bf02392882 · Cited by 226
Culturing Microalgae in Outdoor Ponds
Borowitzka, M. A. (2005) · Algal Culturing Techniques · doi:10.1016/b978-012088426-1/50015-9 · Cited by 226
Cohen, Z. (2002) · CRC Press eBooks · doi:10.1201/9781482295306 · Cited by 217
Neto, P. R. D. C., Rossi, L. F. S., Zagonel, G. F., & Ramos, L. P. (2000) · Química Nova · doi:10.1590/s0100-40422000000400017 · Cited by 205
TOTAL LIPID PRODUCTION OF THE GREEN ALGA NANNOCHLOROPSIS SP. QII UNDER DIFFERENT NITROGEN REGIMES1
Suen, Y., Hubbard, J. S., Holzer, G., & Tornabene, T. G. (1987) · Journal of Phycology · doi:10.1111/j.1529-8817.1987.tb04137.x · Cited by 180
Grünewald, K., Hirschberg, J., & Hagen, C. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m006400200 · Cited by 175
Effects of light intensity, CO2 and nitrogen supply on lipid class composition of Dunaliella viridis
Gordillo, F. J. L., Goutx, M., Figueroa, F. L., & Niell, F. X. (1998) · Journal of Applied Phycology · doi:10.1023/a:1008067022973 · Cited by 170
Extraction of pigments and fatty acids from the green alga Scenedesmus obliquus (Chlorophyceae)
Wiltshire, K. H., Boersma, M., Möller, A., & Buhtz, H. (2000) · Aquatic Ecology · doi:10.1023/a:1009911418606 · Cited by 169
Oxidative stability of biodiesel from soybean oil fatty acid ethyl esters
Ferrari, R. A., Oliveira, V. D. S., & Scabio, A. (2005) · Scientia Agricola · doi:10.1590/s0103-90162005000300014 · Cited by 168
López, M. C. G.-M., Río, E. D., López, J. L. C., Acién, F. G., Fernández-Sevilla, J. M., Rivas, J., Guerrero, M. G., & Grima, E. M. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2005.11.010 · Cited by 154
Kyriakidis, N. B., & Katsiloulis, T. (2000) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-000-0193-3 · Cited by 149
Siron, R., Giusti, G., & Berland, B. R. (1989) · Marine Ecology Progress Series · doi:10.3354/meps055095 · Cited by 144
Reassessment of lipid composition of the diatom, Skeletonema costatum
Bergé, J. P. (1995) · Phytochemistry · doi:10.1016/0031-9422(94)00156-n · Cited by 142
Yuan, J., & Chen, F. (2000) · Food Chemistry · doi:10.1016/s0308-8146(99)00219-8 · Cited by 137
Cerón, M. C., García‐Malea, M. C., Rivas, J., Acién, F. G., Fernández-Sevilla, J. M., Río, E. D., Guerrero, M. G., & Molina, E. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0743-5 · Cited by 134
Zhekisheva, M., Zarka, A., Khozin‐Goldberg, I., Cohen, Z., & Boussiba, S. (2005) · Journal of Phycology · doi:10.1111/j.0022-3646.2005.05015.x · Cited by 127
Cifuentes, A., GONZÁLEZ, M. A., VARGAS, S., Hoeneisen, M., & González, N. (2003) · Biological Research · doi:10.4067/s0716-97602003000300006 · Cited by 115
EFFECT OF TEMPERATURE AND IRRADIANCE ON GROWTH OF HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE)1
Fan, L., Vonshak, A., & Boussiba, S. (1994) · Journal of Phycology · doi:10.1111/j.0022-3646.1994.00829.x · Cited by 104
Damiani, M. C., Leonardi, P. I., Pieroni, O., & Cáceres, E. J. (2006) · Phycologia · doi:10.2216/05-27.1 · Cited by 97
Self-citationOleic acid is the main fatty acid related with carotenogenesis in Dunaliella salina
Mendoza, H. M., Martel, A., del Río, M. J., & Reina, G. G. (1999) · Springer eBooks · doi:10.1007/978-94-011-4449-0_65 · Cited by 82
Ultrastructural study and lipid formation of Isochrysis sp. CCMP1324
Liu, C., & Lin, L. (2001) · Botanical studies · Cited by 78
García‐Malea, M. C., Brindley, C., Río, E. D., Acién, F. G., Fernández-Sevilla, J. M., & Molina, E. (2005) · Biochemical Engineering Journal · doi:10.1016/j.bej.2005.04.007 · Cited by 76
Cysewski, G. R., & Lorenz, R. T. (2003) · doi:10.1002/9780470995280.ch14 · Cited by 66
Harker, M., Tsavalos, A. J., & Young, A. J. (1995) · Journal of Applied Phycology · doi:10.1007/bf00003797 · Cited by 65
A two-stage solar photobioreactor for cultivation of microalgae based on solar concentrators
Masojídek, J., Sergejevová, M., Rottnerová, K., Jirka, V., Korečko, J., Kopecký, J., Zaťková, I., Torzillo, G., & Štys, D. (2008) · Journal of Applied Phycology · doi:10.1007/s10811-008-9324-6 · Cited by 48
Zhukova, N. V., & Titlyanov, E. A. (2006) · Botanica Marina · doi:10.1515/bot.2006.041 · Cited by 34
Carotenogenesis in the green alga Haematococcus pluvialis: Cellular physiology and stress response
Boussiba, S. (2000) · Physiologia Plantarum · doi:10.1034/j.1399-3054.2000.108002111x./ · Cited by 31
Norman, H. A., & Thompson, G. A. (1985) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(85)90490-4 · Cited by 27
Problems in developing the biotechnology of algal biomass production
Vonshak, A., & RICHMOND, A. E. (1985) · Springer eBooks · doi:10.1007/978-94-009-5111-2_10 · Cited by 23
Omega-3 fatty acid composition and biological activity of three microalgae species
Rosa, A., Deidda, D., Serra, A., Deiana, M., Dessì, M., & Pompei, R. (2005) · UNICA IRIS Institutional Research Information System (University of Cagliari) · Cited by 22
Problems in developing the biotechnology of algal biomass production
Vonshak, A., & RICHMOND, A. E. (1985) · Plant and Soil · doi:10.1007/bf02182239 · Cited by 20
Oxidative Stability of Biodiesel
(2005) · AOCS Publishing eBooks · doi:10.1201/9781439822357.ch6.4 · Cited by 17
Effects of X-Rays on the Physico-Chemical Properties of Soybean Oil.
Agba, E. H., & Ibanga, E. J. (2008) · Cited by 1
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- Hu, 2006, Biodiesel from algae: lessons learned over the past 60 years and future perspectives, J. Phycol., 42, 12
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Shah, M. M. R., Liang, Y., Cheng, J. J., & Daroch, M. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.00531 · Cited by 940
Markou, G., & Nerantzis, E. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.07.011 · Cited by 815
Renewable fuels from algae: An answer to debatable land based fuels
Singh, A., Nigam, P. S. N., & Murphy, J. D. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.032 · Cited by 661
Maltsev, Y., Maltseva, K., Kulikovskiy, M., & Maltseva, S. (2021) · Biology · doi:10.3390/biology10101060 · Cited by 511
Metadata
- Authors
- Damiani, M. C., Popovich, C. A., Constenla, D., & Leonardi, P. I.
- Affiliations
- Universidad Nacional del Sur (AR)
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Corresponding author
- Leonardi, Patricia I.
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2010-06
- Volume
- 101
- Issue
- 11
- Pages
- 3801-3807
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2043948111
- Cited by
- 301
- References
- 72
- Citations by year
- 2026: 10 · 2025: 20 · 2024: 13 · 2023: 18 · 2022: 25 · 2021: 28 · 2020: 27 · 2019: 16 · 2018: 12 · 2017: 17 · 2016: 22 · 2015: 17 · 2014: 22 · 2013: 18 · 2012: 20
- FWCI
- 8.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- CONICET Digital (CONICET) (submittedVersion, cc-by-nc-sa)
- Funders
- Consejo Nacional de Investigaciones Científicas y Técnicas (PIP 112, PIP 112-200801-00234)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- Haematococcus pluvialis, biodiesel feedstock, microalgal lipid content, fatty acid composition, nitrogen starvation, high light stress, neutral lipids, polyunsaturated fatty acids, microalgae growth rate
- MeSH terms
- Chlorophyta, Chromatography, Gas, Fatty Acids, Lipids, Microscopy, Electron, Transmission, Biofuels
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