Evaluation of Cell-Disruption Effects of Pulsed-Electric-Field Treatment of Synechocystis PCC 6803
Abstract
In order to use Synechocystis PCC 6803 as feedstock of nonpetroleum-based diesel fuel, pulsed electric field (PEF) technology was used for cell disruption prior to extraction of intracellular lipids. Severe cell disruption was evident after PEF treatment, especially with treatment intensity (TI) > 35 kWh/m(3). Temperature increase during the treatment brought about most of the destruction of autofluorescence compounds, as well as a fraction of inactivation and the destruction of the plasma and thylakoid membranes. However, the forces associated with the pulsing electric field caused significant damage to the plasma membrane, cell wall, and thylakoid membrane, and it even led to complete disruption of some cells into fragments, which resulted in biomass loss. Treatment by PEF enhanced the potential for the low-toxicity solvent isopropanol to access lipid molecules during subsequent solvent extraction, leading to lower usage of isopropanol for the same extraction efficiency. Thus, PEF shows promise for lowering the costs and environmental effects of the lipid-extraction step.
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Mechanical methods do not contaminate the environment but impose higher costs due to energy consumption.
“They do not contaminate the environment, but they do impose higher costs, due to the consumption of energy [116, 117].”
From The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products (2018) · cites this paper as [117]
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- Authors
- Sheng, J., Vannela, R., & Rittmann, B. E.
- Affiliations
- Arizona State University (US)
- Corresponding author
- Sheng, Jie
- ORCID
- Rittmann, Bruce E.
- Venue
- Environmental Science & Technology
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-03-23
- Volume
- 45
- Issue
- 8
- Pages
- 3795-3802
- ISSN
- 0013-936X (ISSN-L)
- 1520-5851
- OpenAlex
- W2027555951
- Cited by
- 144
- References
- 38
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 6 · 2023: 6 · 2022: 7 · 2021: 10 · 2020: 11 · 2019: 17 · 2018: 15 · 2017: 14 · 2016: 11 · 2015: 20 · 2014: 10 · 2013: 8 · 2012: 4
- FWCI
- 6.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Environmental Science & Technology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Plasma Applications and Diagnostics
- Keywords
- pulsed electric field, cell disruption, Synechocystis sp. PCC 6803, microalgae, cell membrane damage, thylakoid membranes, treatment intensity, biomass loss
- MeSH terms
- Cell Membrane, Disinfection, Electricity, Chemical Fractionation, Lipids, Solvents, 2-Propanol, Synechocystis, Microbial Viability, Biofuels
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