Outdoor pilot-scale production of Nannochloropsis gaditana: Influence of culture parameters and lipid production rates in tubular photobioreactors
Abstract
This work studied outdoor pilot scale production of Nannochloropsis gaditana in tubular photobioreactors. The growth and biomass composition of the strain were studied under different culture strategies: continuous-mode (varying nutrient supply and dilution rate) and two-stage cultures aiming lipid enhancement. Besides, parameters such as irradiance, specific nitrate input and dilution rate were used to obtain models predicting growth, lipid and fatty acids production rates. The range of optimum dilution rate was 0.31-0.351/day with maximum biomass, lipid and fatty acids productivities of 590, 110 and 66.8 mg/l day, respectively. Nitrate limitation led to an increase in lipid and fatty acids contents (from 20.5% to 38.0% and from 16.9% to 23.5%, respectively). Two-stage culture strategy provided similar fatty acids productivities (56.4 mg/l day) but the neutral lipids content was doubled.
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In the PBR system, an optimum biomass productivity of 0.59 g/L was found in a dilution range of 0.31-0.35 g/L, with irradiance of 475 μE/m2.s.
“Optimum biomass productivity of 0.59 g/L was found in a dilution range of 0.31–0.35 g/L, with irradiance of 475 µE/m2.s.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
The models were considered a powerful tool for the improvement and scale-up of microalgae production for biofuels.
“The models were considered a powerful tool for the improvement and scale-up of microalgae production for biofuels.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
These parameters were applied to develop models used in describing Nannochloropsis gaditana biomass productivity and lipid yield.
“These parameters were applied to develop models used in describing Nannochloropsis gaditana biomass productivity and lipid yield.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
Parameters considered to affect lipid productivity were dilution rate of nutrient supply (N and P), irradiance as a measure of light availability and temperature.
“Parameters considered to affect lipid productivity were dilution rate of nutrient supply (N and P), irradiance as a measure of light availability and temperature.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
FA and lipid contents decreased with dilution rate increase, indicating no accumulation enhancement occurred because maximal biomass productivity was the same as maximum for FA and lipid compounds.
“FA and lipid contents decreased with dilution rate increase, indicating no accumulation enhancement occurred because maximal biomass productivity was the same as maximum for FA and lipid compounds.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
San Pedro et al. investigated the cultivation of the microalgae Nannochloropsis gaditana using open raceway ponds, photobioreactors and flat-panel bioreactors in outdoor pilot scale studies.
“San Pedro et al. [81,82,83] investigated the cultivation of the microalgae Nannochloropsis gaditana using ORP, PBR and flat-panel bioreactor (FPB) in outdoor pilot scale studies.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
An increase in protein content was observed with higher dilution rates, indicating nitrogen conversion to proteins.
“An increase in protein content was observed with higher dilution rates, indicating nitrogen conversion to proteins.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
From the three cultivation systems it was possible to optimize growth conditions in terms of the culture medium, temperature and dilution rate with Nannochloropsis gaditana using the developed models.
“From the three cultivation systems it was possible to optimize growth conditions in terms of the culture medium, temperature and dilution rate with Nannochloropsis gaditana using the developed models.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
The studies allowed the establishment of the influence of culture conditions and reactor design and orientation on Nannochloropsis gaditana productivity.
“These studies allowed the establishment of the influence of culture conditions and reactor design and orientation on Nannochloropsis gaditana productivity.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
The importance of the models lied in their reliability and ease of use for calculating large-scale biofuel requirements when considering the productivity of microalgae, especially in terms of lipid production.
“The importance of the models lied in their reliability and ease of use for calculating large-scale biofuel requirements when considering the productivity of microalgae, especially in terms of lipid production.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [82]
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- Authors
- San Pedro, A., González-López, C. V., Acién, F. G., & Molina-Grima, E.
- Affiliations
- University of Almería (ES)
- ORCID
- Acién, F.G.
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 169
- Pages
- 667-676
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2004787344
- Cited by
- 110
- References
- 35
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 7 · 2023: 6 · 2022: 12 · 2021: 8 · 2020: 16 · 2019: 15 · 2018: 10 · 2017: 9 · 2016: 11 · 2015: 9
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- ENDESA S.A.
- Ministerio de Economía y Competitividad (Spain)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- Nannochloropsis gaditana, microalgae cultivation, tubular photobioreactor, lipid production, fatty acid production, dilution rate, nitrate limitation, irradiance, two-stage culture, continuous culture, growth models, microalgal biomass
- MeSH terms
- Biotechnology, Fatty Acids, Lipids, Pilot Projects, Biomass, Cell Culture Techniques, Stramenopiles, Photobioreactors
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