Integrated bioethanol and protein production from brown seaweed Laminaria digitata
Abstract
A wild-growing glucose-rich (i.e. 56.7% glucose content) brown seaweed species Laminaria digitata, collected from the North Coast of Denmark in August 2012, was used as the feedstock for an integrated bioethanol and protein production. Glutamic acid and aspartic acid are the two most abundant amino acids in the algal protein, both with proportional content of 10% in crude protein. Only minor pretreatment of milling was used on the biomass to facilitate the subsequent enzymatic hydrolysis and fermentation. The Separate Hydrolysis and Fermentation (SHF) resulted in obviously higher ethanol yield than the Simultaneous Saccharification and Fermentation (SSF). High conversion rate at maximum of 84.1% glucose recovery by enzymatic hydrolysis and overall ethanol yield at maximum of 77.7% theoretical were achieved. Protein content in the solid residues after fermentation was enriched by 2.7 fold, with similar distributions of amino acids, due to the hydrolysis of polymers in the seaweed cell wall matrix.
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Our results are similar to Hou et al. (2015), who produced 14.7 and 20.7 g/L ethanol (50.5 and 70.6% conversion yield) using SSF and SHF on Laminaria digitata with S. cerevisiae.
“Our results are similar to Hou et al. (2015), who worked under two fermentation strategies: SSF and SHF on Laminaria digitata as substrate, using a commercial yeast S. cerevisiae , they produced 14.7 and 20.7 g/L ethanol (corresponding to 50.5 and 70.6 % of conversion yield) using SSF and SHF, respectively.”
From High-pressure technology for Sargassum spp biomass pretreatment and fractionation in the third generation of bioethanol production (2021) · cites this paper as Hou et al. (2015)
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- Authors
- Hou, X., Hansen, J. H., & Bjerre, A.-B.
- Affiliations
- Danish Technological Institute (DK)
- Corresponding author
- Hou, Xiaoru
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 197
- Pages
- 310-317
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W1482806932
- Cited by
- 105
- References
- 34
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 12 · 2023: 7 · 2022: 14 · 2021: 19 · 2020: 14 · 2019: 7 · 2018: 7 · 2017: 11 · 2016: 6 · 2015: 1
- FWCI
- 4.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Danish Council for Strategic Research
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- Laminaria digitata, brown algae, bioethanol production, algal proteins, separate hydrolysis and fermentation, simultaneous saccharification and fermentation, enzymatic hydrolysis, glutamate, aspartic acid, seaweed cell wall, ethanol yield, protein enrichment
- MeSH terms
- Ethanol, Denmark, Fermentation, Glucose, Hydrolysis, Laminaria, Seaweed, Biomass, Algal Proteins
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