Multiple-response optimization of the acidic treatment of the brown alga Ecklonia radiata for the sequential extraction of fucoidan and alginate
Abstract
The aim of this study was to optimize the acidic treatment of the brown alga Ecklonia radiata in order to extract fucoidan and facilitate the efficient sequential extraction of alginates. Response surface methodology was used to determine the effects of the temperature, pH, and duration of the acidic treatment on fucoidan yield, alginate extractability, and the molecular weight of sequentially extracted alginates. Desirability functions were then used to predict the best overall combinations of responses. The most desirable compromise allowed for the recovery of a fucoidan-rich fraction with a yield of 3.75% (w/w of alga) and the sequential extraction of alginates having an average molecular weight of 730kDa at a yield of 44% (w/w of alga), with low cross-contamination between the products. The optimized acidic treatment could form the basis of an industrial biorefinery process for the production of both fucoidan and alginate.
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Box-Behnken design was used to optimize pH, temperature and time of HCl treatment of defatted Ecklonia radiata to obtain high yield of fucoidan and alginates and low alginate degradation.
“Recently, Box-Behnken design was used to optimize the pH, temperature and time of application of HCl treatment to a defatted biomass of Ecklonia radiata to obtain a high yield of both fucoidan and alginates and low degradation of alginates, measured by their molecular weight characteristics of each extract obtained”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Lorbeer et al., 2015
Studies optimizing macroalgal polysaccharide extraction mainly used Box-Behnken and central composite designs to analyse at least three factors.
“Studies on the optimization of one or more steps of the extraction processes of polysaccharides from macroalgae mainly used Box-Behnken and central composite designs to analyse at least 3 or more factors affecting the extraction process”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Lorbeer et al., 2015
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- Authors
- Lorbeer, A. J., Lahnstein, J., Bulone, V., Nguyen, T., & Zhang, W.
- Affiliations
- Flinders University (AU)
- The University of Adelaide (AU)
- Corresponding author
- Zhang, Wei
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 197
- Pages
- 302-309
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W1641289213
- Cited by
- 116
- References
- 30
- Citations by year
- 2026: 12 · 2025: 13 · 2024: 22 · 2023: 15 · 2022: 9 · 2021: 7 · 2020: 10 · 2019: 8 · 2018: 7 · 2017: 6 · 2016: 6
- FWCI
- 9.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
- Funders
- Premier’s Research and Industry Fund of the South Australian Government
- Qingdao Gather Great Ocean Seaweed Industry Co., Ltd
- Australian Kelp Products Pty Ltd
- Flinders University
- Australian Research Council
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Keywords
- Ecklonia radiata, fucoidan, alginate, acid treatment, sequential extraction, brown algae, response surface methodology, desirability function, molecular weight, biorefinery, seaweed polysaccharides
- MeSH terms
- Phaeophyceae, Alginates, Chemical Fractionation, Hexuronic Acids, Molecular Weight, Polysaccharides, Glucuronic Acid
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