A novel two-phase bioprocess for the production of Arthrospira (Spirulina) Maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions
Abstract
A two-phase outdoor cultivation bioprocess for Arthrospira maxima LJGR1 combined with phycocyanin induction in concentrated cultures under controlled conditions was evaluated using a modified low-cost Zarrouk medium. Growth was monitored during 4 cycles in 2018 and 4 cycles in 2019. Biomass was harvested and concentrated using membrane technology at the end of each cycle for further phycocyanin induction using blue LED light (controlled conditions, 24 h). The highest biomass productivity was observed during spring and summer cycles (13.63-18.97 gDWm-2 d-1); during mid-fall and mid-end fall, a decrease was observed (9.93-7.76 gDWm-2 d-1). Under favorable growth conditions, phycocyanin induction was successful. However, during cycles with unfavorable growth condition, phycocyanin induction was not observed. Reactive-grade phycocyanin (3.72 ± 0.14) was recovered and purified using microfiltration and ultrafiltration technologies.
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Garcia-Lopez et al. found complicated reactions of Arthrospira cultures under external circumstances, owing to a variety of variables impacting phycocyanin production metabolism.
“Similarly, Garcia-Lopez et al. [43] found complicated reactions of Arthrospira cultures under external circumstances, owing to a variety of variables impacting phycocyanin production metabolism [43].”
From Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content (2022) · cites this paper as [43]
Phycocyanin levels are influenced by a variety of environmental conditions, including light intensity and quality, changes in the light route, and reactor temperatures, due to the diverse contributions of ecological conditions in outdoor cultures.
“Phycocyanin levels are influenced by a variety of environmental conditions, including light intensity and quality, changes in the light route, and reactor temperatures, due to the diverse contributions of ecological conditions in outdoor cultures.”
From Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content (2022) · cites this paper as [43]
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- Authors
- García-López, D. A., Olguín, E. J., González-Portela, R. E., Sánchez-Galván, G., De Philippis, R., Lovitt, R. W., Llewellyn, C. A., Fuentes-Grünewald, C., & Parra Saldívar, R.
- Affiliations
- Instituto de Ecología (MX)
- Swansea University (GB)
- Corresponding author
- Olguín, E.J.
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2020-02
- Volume
- 298
- Pages
- 122548
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2994443626
- Semantic Scholar
- e70d29738e61a2e475b39425bf2680f731e7a5e0
- Cited by
- 69
- References
- 72
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 9 · 2023: 22 · 2022: 8 · 2021: 10 · 2020: 8
- FWCI
- 1.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Florence Research (University of Florence) (submittedVersion)
- Funders
- U. K. Newton Fund and the National Council for Science and Technology of Mexico (0268792)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- Arthrospira maxima, Spirulina, phycocyanin production, two-phase cultivation, open-field cultivation, seasonal variation, blue LED light, Zarrouk medium, microfiltration, ultrafiltration, membrane separation, biomass productivity, cyanobacteria
- MeSH terms
- Light, Phycocyanin, Biomass, Spirulina
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