Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Open accessAbstract
Although osteocytes have historically been viewed as quiescent cells, it is now clear that they are highly active cells in bone and play key regulatory roles in diverse skeletal functions, including mechanotransduction, phosphate homeostasis and regulation of osteoblast and osteoclast activity. Three dimensional imaging of embedded osteocytes and their dendritic connections within intact bone specimens can be quite challenging and many of the currently available methods are actually imaging the lacunocanalicular network rather than the osteocytes themselves. With the explosion of interest in the field of osteocyte biology, there is an increased need for reliable ways to image these cells in live and fixed bone specimens. Here we report the development of reproducible methods for 2D and 3D imaging of osteocytes in situ using multiplexed imaging approaches in which the osteocyte cell membrane, nucleus, cytoskeleton and extracellular matrix can be imaged simultaneously in various combinations. We also present a new transgenic mouse line expressing a membrane targeted-GFP variant selectively in osteocytes as a novel tool for in situ imaging of osteocytes and their dendrites in fixed or living bone specimens. These methods have been multiplexed with a novel method for labeling of the lacunocanalicular network using fixable dextran, which enables aspects of the osteocyte cell structure and lacunocanalicular system to be simultaneously imaged. The application of these comprehensive approaches for imaging of osteocytes in situ should advance research into osteocyte biology and function in health and disease.
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In osteocytes, EVs can be observed in proximity of the osteocytic network.
“In osteocytes, EVs can be observed in proximity of the osteocytic network.37”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [37]
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- Authors
- Kamel-ElSayed, S. A., Tiede-Lewis, L. M., Lu, Y., Veno, P. A., & Dallas, S. L.
- Affiliations
- Oakland University (US)
- University of Missouri–Kansas City (US)
- Corresponding author
- Dallas, Sarah L.
- Venue
- Bone
- Publisher
- Elsevier BV
- Published
- 2015-07
- Volume
- 76
- Pages
- 129-140
- ISSN
- 1873-2763 (ISSN-L)
- 8756-3282
- PMC
- PMC4591054
- OpenAlex
- W2047035939
- Cited by
- 87
- References
- 41
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 5 · 2023: 8 · 2022: 10 · 2021: 4 · 2020: 12 · 2019: 11 · 2018: 7 · 2017: 8 · 2016: 9 · 2015: 1
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Update notices
- erratum (2016-02-01)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Bone (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH (R21-AR054449, RO1-AR051517, S10RR027668, PO1-AR46798)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- osteocytes, multiplexed imaging, 3D imaging, transgenic mice, cytoskeleton, extracellular matrix, mechanotransduction, phosphate homeostasis
- MeSH terms
- Animals, Diagnostic Imaging, Mice, Inbred C57BL, Mice, Transgenic, Osteocytes, Extracellular Matrix Proteins, Green Fluorescent Proteins, Mice
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