Cell line IDG-SW3 replicates osteoblast-to-late-osteocyte differentiation in vitro and accelerates bone formation in vivo
Open accessAbstract
Osteocytes are the most abundant cells in bone yet are the most challenging to study because they are embedded in a mineralized matrix. We generated a clonal cell line called IDG-SW3 (for Immortomouse/Dmp1-GFP-SW3) from long-bone chips from mice carrying a Dmp1 promoter driving GFP crossed with the Immortomouse, which expresses a thermolabile SV40 large T antigen regulated by interferon γ (IFN-γ). Cells from these mice can be expanded at 33 °C in the presence of IFN-γ and then allowed to resume their original phenotype at 37 °C in the absence of IFN-γ. IDG-SW3 cells are Dmp1-GFP− and T antigen+ under immortalizing conditions but Dmp1-GFP+ and T antigen− under osteogenic conditions. Like osteoblasts, they express alkaline phosphatase and produce and mineralize a type 1 collagen matrix containing calcospherulites. Like early osteocytes, they express E11/gp38, Dmp1, MEPE, and Phex. Like late osteocytes, they develop a dendritic morphology and express SOST/sclerostin and fibroblast growth factor 23 (FGF-23), regulated by parathyroid hormone (PTH) and 1,25-dihydroxyvitamin D3. When cultured on 3D matrices, they express Dmp1-GFP and sclerostin. When the 3D cultures are implanted in calvarial defects in vivo, they accelerate bone healing. This cell line should prove useful for studying osteoblast-to-osteocyte transition, mechanisms for biomineralization, osteocyte function, and regulation of SOST/sclerostin and FGF-23. © 2011 American Society for Bone and Mineral Research
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In the IDG-SW3 dataset, days 3, 14 and 35 represent osteoblast, early osteocyte and mature osteocyte stages, respectively.
“Data from days 3, 14 and 35 represent osteoblast, early osteocyte and mature osteocyte stages, respectively86.”
From Osteocyte transcriptome mapping identifies a molecular landscape controlling skeletal homeostasis and susceptibility to skeletal disease (2021) · cites this paper as [86]
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Rutkowski, J. M., Pastor, J. V., Sun, K., Park, S. K., Bobulescu, I. A., Chen, C. T., Moe, O. W., & Scherer, P. E. (2016) · Kidney International · doi:10.1016/j.kint.2016.09.016 · Cited by 57 · see citing works
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- Authors
- Woo, S. M., Rosser, J., Dusevich, V., Kalajzic, I., & Bonewald, L. F.
- Affiliations
- University of Missouri–Kansas City (US)
- Department of Reconstructive Sciences, University of Connecticut Health Center, School of Dental Medicine, Farmington, CT, USA
- Corresponding author
- Bonewald, Lynda F
- ORCID
- Bonewald, Lynda F
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-07-06
- Volume
- 26
- Issue
- 11
- Pages
- 2634-2646
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- PMC
- PMC3192242
- OpenAlex
- W1985564086
- Semantic Scholar
- 5073feae7cce21d7166d2f0cfd327c76e4bcb852
- Cited by
- 243
- References
- 39
- Citations by year
- 2026: 9 · 2025: 14 · 2024: 16 · 2023: 17 · 2022: 18 · 2021: 24 · 2020: 22 · 2019: 26 · 2018: 18 · 2017: 14 · 2016: 15 · 2015: 19 · 2014: 14 · 2013: 7 · 2012: 9
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Bone and Mineral Research (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- American Association of Endodontists Foundation
- National Institutes of Health
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R03AR053275, P01AR046798)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- osteocytes, SV40 large T antigen, interferon-gamma, sclerostin, fibroblast growth factor 23, parathyroid hormone, 1,25-dihydroxyvitamin D3, bone regeneration, calvarial defect, biomineralization, type I collagen, osteocyte function
- MeSH terms
- Fibroblast Growth Factor-23, Animals, Antigens, Polyomavirus Transforming, Bone and Bones, Calcification, Physiologic, Calcium, Cell Differentiation, Cell Line, Cell Movement, Extracellular Space, Gene Expression Regulation, Microspheres, Osteocytes, Osteogenesis, Time Factors, Transcription Factors, Wound Healing, Biomarkers, Gene Expression Profiling, Fibrillar Collagens, Cell Proliferation, Green Fluorescent Proteins, Mice, Rats
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