The GluN2B subunit of N-methy-D-asparate receptor regulates the radial migration of cortical neurons in vivo
Abstract
The formation of layered structure of the mammalian neocortex requires a fine organized migration of post-mitotic neurons during early development. However, whether the radial migration is regulated by NMDA receptor and specific subunits remains contradictory and unknown. Here, we reported that in the developing rat cortex, migration of presumptive layer II/III neurons to their deserved destination was regulated by NMDA receptors with GluN2B but not GluN2A subunit. Using in utero electroporation of small interference RNA (siRNA) of distinct NMDA receptor subunits, we found that knockdown GluN1 and GluN2B subunits dramatically delayed the neuronal migration to proper layer II/III, while improperly stayed at lower layers or even the germinal regions, without changing the cell fate. In contrast, knockdown of GluN2A subunit did not impair the neuronal migration. Additionally, the ecotopic neurons by GluN2B RNAi developed to well dendritic differentiation, while the ones by GluN1 RNAi still kept morphology of migrating neurons. Therefore, GluN2B subunit of NMDA receptor plays an essential role in regulating proper neuronal migration and cortical lamination.
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In utero knockdown of Grin2b delays cortical neuron migration and increases dendritic length and branching.
“In utero knockdown of Grin2b results in delayed migration of cortical neurons, together with increased dendritic length and branching [179].”
From Autism spectrum disorder: neuropathology and animal models (2017) · cites this paper as [179]
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- Authors
- Jiang, H., Jiang, W., Zou, J., Wang, B., Yu, M., Pan, Y., Lin, Y., Mao, Y., & Wang, Y.
- Affiliations
- Tongji University (CN)
- Shanghai Jiao Tong University (CN)
- Pennsylvania State University (US)
- Corresponding author
- Wang, Yonggang
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2015-06
- Volume
- 1610
- Pages
- 20-32
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W1965333604
- Cited by
- 27
- References
- 62
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 2 · 2022: 5 · 2021: 1 · 2020: 3 · 2019: 4 · 2018: 4 · 2017: 2
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- GluN2B, NMDA receptor, radial neuronal migration, cortical lamination, GluN1, GluN2A, neocortex development, siRNA knockdown, dendritic differentiation, in utero electroporation
- MeSH terms
- Animals, Animals, Newborn, Cell Movement, Cell Survival, Cells, Cultured, Cerebral Cortex, Neurons, Receptors, N-Methyl-D-Aspartate, Rats, Sprague-Dawley, Electroporation, RNA Interference, Neurogenesis, Gene Knockdown Techniques, Solute Carrier Family 12, Member 2
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