Development and effectiveness of pseudotyped SARS-CoV-2 system as determined by neutralizing efficiency and entry inhibition test in vitro
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With the development of the COVID-19 epidemic, there is an urgent need to establish a system for determining the effectiveness and neutralizing activity of vaccine candidates in biosafety level 2 (BSL-2) facilities. Previously, researchers had developed a pseudotyped virus system for SARS-CoV and MERS-CoV, based on HIV-1 core, bearing virus spike protein. During the development of a pseudotyped SARS-CoV-2 system, a eukaryotic expression plasmid expressing SARS-CoV-2 spike (S) protein was constructed and then co-transfected with HIV-1 based plasmid which containing the firefly luciferase reporter gene, into HEK293T cells to prepare the pseudotyped SARS-CoV-2 virus (ppSARS-2). We have successfully established the pseudotyped SARS-CoV-2 system for neutralization and entry inhibition assays. Huh7.5 cell line was found to be the most susceptible to our pseudotyped virus model. Different levels of neutralizing antibodies were detected in convalescent serum samples of COVID-19 patients using ppSARS-2. The recombinant, soluble, angiotensin-converting enzyme 2 protein was found to inhibit the entry of ppSARS-2 in Huh7.5 cells effectively. Furthermore, the neutralization results for ppSARS-2 were consistent with those of live SARS-CoV-2 and determined using the serum samples from convalescent patients. In conclusion, we have developed an easily accessible and reliable tool for studying the neutralizing efficiency of antibodies against SARS-CoV-2 and the entry process of the virus in a BSL-2 laboratory.
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Several studies have shown that authentic virus and pseudovirus neutralization correlate quite well.
“Several studies have now shown that authentic virus and pseudovirus neutralization correlate quite well (16, 86, 87).”
From mRNA vaccination boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection (2021) · cites this paper as [87]
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- Authors
- Yang, R., Huang, B., A, R., Li, W., Wang, W., Deng, Y., & Tan, W.
- Affiliations
- Chinese Center For Disease Control and Prevention (CN)
- National Institute of Biological Sciences, Beijing (CN)
- Corresponding author
- Deng, Yao
- Tan, Wenjie
- Venue
- Biosafety and Health
- Publisher
- Elsevier BV
- Published
- 2020-12
- Volume
- 2
- Issue
- 4
- Pages
- 226-231
- ISSN
- 2590-0536 (ISSN-L)
- PMC
- PMC7442068
- OpenAlex
- W3080470999
- Cited by
- 70
- References
- 25
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 6 · 2023: 14 · 2022: 18 · 2021: 21 · 2020: 5
- FWCI
- 1.90
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Biosafety and Health (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Key Research and Development Program of China (2016YFD0500301, 2020YFC0842100)
- Chinese Center for Disease Control and Prevention
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
- Keywords
- SARS-CoV-2, pseudovirus, neutralizing antibodies, spike protein, Huh7.5 cells, ACE2, vaccine candidate evaluation, neutralization test
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