Evaluation of healing outcomes combining a novel polymer formulation with autologous skin cell suspension to treat deep partial and full thickness wounds in a porcine model: a pilot study
Open accessAbstract
Autologous skin cell suspensions (ASCS) can treat burns of varying depths with the advantage of reduced donor site wound burden. The current standard primary dressing for ASCS is a nonabsorbant, non-adherent, perforated film (control) which has limited conformability over heterogeneous wound beds and allows for run-off of the ASCS. To address these concerns, a novel spray-on polymer formulation was tested as a potential primary dressing in porcine deep partial thickness (DPT) and full thickness (FT) wounds. It was hypothesized that the polymer would perform as well as control dressing when evaluating wound healing and scarring. DPT or FT wounds were treated with either a spray-on poly(lactic-co-glycolic acid) (PLGA) and poly(lactide-co-caprolactone) (PLCL) formulation or control ASCS dressings. Throughout the experimental time course (to day 50), we found no significant differences between polymer and control wounds in % re-epithelialization, graft-loss, epidermal or dermal thickness, or % dermal cellularity in either model. Pigmentation, erythema, elasticity, and trans-epidermal water loss (TEWL), were not significantly altered between the treatment groups, but differences between healing wounds/scars and un-injured skin were observed. No cytotoxic effect was observed in ASCS incubated with the PLGA and PLCL polymers. These data suggest that the novel spray-on polymer is a viable option as a primary dressing, with improved ease of application and conformation to irregular wounds. Polymer formulation and application technique should be a subject of future research.
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Fibers fabricated via solution blow spinning exhibit minimal solvent residues, have diameters ranging from the nanoscale to the microscale, and PLCL materials can be fabricated into various scaffolds via solution blow spinning for diverse biomedical fields.
“The results showed that fibers fabricated via the SBS method exhibit minimal solvent residues, the fiber scaffolds had diameters ranging from the nanoscale to the microscale, and PLCL materials could be fabricated into various scaffolds via the SBS method for applications in diverse biomedical fields [84–87].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [87]
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- Authors
- Carney, B. C., Oliver, M. A., Erdi, M., Kirkpatrick, L. D., Tranchina, S. P., Rozyyev, S., Keyloun, J. W., Saruwatari, M. S., Daristotle, J. L., Moffatt, L. T., Kofinas, P., Sandler, A. D., & Shupp, J. W.
- Affiliations
- Georgetown University (US)
- MedStar Health Research Institute (US)
- University of Maryland, College Park (US)
- Children's National (US)
- MedStar Washington Hospital Center (US)
- Koch Institute for Integrative Cancer Research At MIT (US)
- Corresponding author
- Shupp, Jeffrey W.
- ORCID
- Venue
- Burns
- Publisher
- Elsevier BV
- Published
- 2022-12
- Volume
- 48
- Issue
- 8
- Pages
- 1950-1965
- ISSN
- 0305-4179 (ISSN-L)
- PMC
- PMC9300815
- OpenAlex
- W4207083894
- Cited by
- 15
- References
- 30
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 2 · 2023: 4
- FWCI
- 1.19
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Burns (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM141132)
- National Institute of Biomedical Imaging and Bioengineering (R01EB019963)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Epidemiology › Burn Injury Management and Outcomes
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- autologous skin cell suspension, burn wound healing, PLGA, PLCL, full-thickness wounds, re-epithelialization, scar formation, porcine model, transepidermal water loss
- MeSH terms
- Animals, Burns, Cicatrix, Pilot Projects, Polymers, Swine, Wound Healing, Skin Transplantation
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