Exosomes derived from human umbilical cord mesenchymal stromal cells deliver exogenous miR-145-5p to inhibit pancreatic ductal adenocarcinoma progression
Abstract
The roles of miRNAs in the development of cancer have made them promising tools for novel therapeutic approaches. However, the successful delivery of miRNAs to cancer cells has been hampered by difficulties in developing an effective and sustainable delivery mechanism. Exosomes are small endogenous membrane vesicles that mediate communication between cells by delivering genetic materials. Thus, given their intrinsic properties, exosomes have been a focus for use as biological delivery vehicles for miRNAs transfer. Whether exosomes can effectively deliver exogenous miRNAs to pancreatic ductal adenocarcinoma (PDAC) cells has not been thoroughly investigated. Here, we used exosomes from human umbilical cord mesenchymal stromal cells (hucMSCs) to deliver exogenous miR-145-5p, which inhibited PDAC cell proliferation and invasion and increased apoptosis and cell cycle arrest, concomitant with decreased Smad3 expression in vitro. Using a mouse model, we also demonstrated that overexpressing miR-145-5p significantly reduced the growth of xenograft tumors in vivo. Our findings provide novel insights that exosomes might be an attractive therapeutic vehicle for the clinical administration of miRNAs in patients with PDAC.
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Exosomes from MSCs with higher miR-1455p display a strong effect on inhibition of pancreatic cancer cells in vitro and in vivo.
“In addition, exosomes from MSCs had a higher amount of miR-1455p were displaying a strong effect on the inhibition of pancreatic cancer cells in vitro and in vivo [52].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [52]
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- Authors
- Ding, Y., Cao, F., Sun, H., Wang, Y., Liu, S., Wu, Y., Cui, Q., Mei, W., & Li, F.
- Affiliations
- Capital Medical University (CN)
- Corresponding author
- Li, Fei
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 2019-02
- Volume
- 442
- Pages
- 351-361
- ISSN
- 0304-3835 (ISSN-L)
- OpenAlex
- W2900319331
- Cited by
- 177
- References
- 40
- Citations by year
- 2026: 9 · 2025: 24 · 2024: 19 · 2023: 33 · 2022: 33 · 2021: 30 · 2020: 18 · 2019: 11
- FWCI
- 5.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cancer Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation, China (81272756)
- Beijing Natural Science Foundation, China (7162076)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- exosomes, miR-145, pancreatic ductal adenocarcinoma, mesenchymal stem cells, Smad3, tumor cell proliferation and invasion, apoptosis, cell cycle arrest, xenograft tumor growth, miRNA-based therapy
- MeSH terms
- Animals, Cell Movement, Humans, Male, Mice, Inbred BALB C, Mice, Nude, Neoplasm Invasiveness, Pancreatic Neoplasms, Umbilical Cord, Antigens, CD, Cadherins, Apoptosis, Carcinoma, Pancreatic Ductal, Xenograft Model Antitumor Assays, MicroRNAs, Cell Line, Tumor, Tumor Burden, Cell Proliferation, Apoptosis Regulatory Proteins, Smad3 Protein, Exosomes, Cell Cycle Checkpoints, Mesenchymal Stem Cells
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