Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata)

Wu, Hao; Zhu, Junxiang; Diao, Wenchao; Wang, Chengrong

doi:10.1016/j.carbpol.2014.07.025

Cited by 169Polysaccharides Composition and ApplicationsPolysaccharides and Plant Cell WallsAgricultural and Biological Research

Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata)

Wu, Hao, Zhu, Junxiang, Diao, Wenchao, Wang, Chengrong·2014·Carbohydrate Polymers

DOI 10.1016/j.carbpol.2014.07.025

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Abstract

An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita moschata polysaccharides (CMCP) was established and the CMCP antioxidant activities were studied. The UAEE operating parameters (extraction temperature, ultrasonic power, pH, and liquid-to-material ratio) were optimized using the central composite design (CCD) and the mass transfer kinetic study in UAEE procedure was used to select the optimal extraction time. Enzymolysis and ultrasonication that were simultaneously conducted was selected as the UAEE synergistic model and the optimum extraction conditions with a maximum polysaccharide yield of 4.33 ± 0.15% were as follows: extraction temperature, 51.5 °C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; and extraction time, 20 min. Evaluation of the antioxidant activity in vitro suggested that CMCP has good potential as a natural antioxidant used in the food or medicine industry because of their high reducing power and positive radical scavenging activity for DPPH radical.

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Wu et al. [133] found that compared with EAE alone and UAE alone, ultrasonic and enzyme action together can increase extraction efficiency of polysaccharides.
“Compared with EAE alone and UAE alone, ultrasonic and enzyme action together can increase the extraction efficiency of polysaccharides.”
From A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies (2023) · cites this paper as [133]

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Seaweed-derived phenolic compounds as diverse bioactive molecules: A review on identification, application, extraction and purification strategies
Sadeghi, A., Rajabiyan, A., Nabizade, N., Nezhad, N. M., & Ahmady, A. Z. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.131147 · Cited by 108 · see citing works
Bioactive Carbohydrates and Peptides in Foods: An Overview of Sources, Downstream Processing Steps and Associated Bioactivities
Hayes, M., & Tiwari, B. K. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms160922485 · Cited by 101 · see citing works
“Application of ultrasound along with enzymes has a potential to enhance extraction yields of polysachararides from various plant matrices including wheat bran [108,109].”
Background
Ultrasound-assisted extraction of R-phycoerythrin from Grateloupia turuturu with and without enzyme addition
Guillard, C. L., Dumay, J., Donnay‐Moreno, C., Bruzac, S., Ragon, J.-Y., Fleurence, J., & Bergé, J.-P. (2015) · Algal Research · doi:10.1016/j.algal.2015.11.002 · Cited by 97 · see citing works
Response Surface Methodology Optimization of Ultrasonic-Assisted Extraction of Acer Truncatum Leaves for Maximal Phenolic Yield and Antioxidant Activity
Yang, L., Yin, P., Fan, H., Xue, Q., Li, K., Li, X., Sun, L., & Liu, Y. (2017) · Molecules · doi:10.3390/molecules22020232 · Cited by 93 · see citing works
“For these reasons, UAE from plant materials has been widely used lately to facilitate extractions of phenols from Chrysanthemum morifolium flower heads [10], Olea europaea fruits [11], Morus nigra must [12], and Curcuma longa rhizomes [13], lipids from rice [14], polysaccharides from pumpkin [15] and mycelial fermenta…”
Background
Ultrasound-Assisted Aqueous Enzymatic Extraction of Oil from Pomegranate Seeds
Goula, A. M., Papatheodorou, A., Karasavva, S., & Kaderides, K. (2016) · Waste and Biomass Valorization · doi:10.1007/s12649-016-9740-9 · Cited by 92 · see citing works
“However, it is very difficult to select the best enzyme for a given oilseed comparing the results reported in different studies, since these studies employ different extraction conditions, such as temperature, time, pH, and particle size [36].”
Background
Study on the structure, antioxidant activity and degradation pattern of polysaccharides isolated from lotus seedpod
Yuan, S., Wang, J., Li, X., Zhu, X., Zhang, Z., & Li, D. (2023) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2023.121065 · Cited by 90 · see citing works
Extraction, purification, structure, modification, and biological activity of traditional Chinese medicine polysaccharides: A review
Xue, H., Li, P., Bian, J., Gao, Y., Sang, Y., & Tan, J. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.1005181 · Cited by 86 · see citing works
Kinetic models for conventional and ultrasound assistant extraction of polyphenols from defatted fresh and distilled grape marc and its main components skins and seeds
Natolino, A., & Porto, C. D. (2020) · Process Safety and Environmental Protection · doi:10.1016/j.cherd.2020.01.009 · Cited by 79 · see citing works
Structural characterization of Lanzhou lily (Lilium davidii var. unicolor) polysaccharides and determination of their associated antioxidant activity
Li, W., Wang, Y., Wei, H., Zhang, Y., Guo, Z., Qiu, Y., Wen, L., & Xie, Z. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10613 · Cited by 74 · see citing works
Experimental and theoretical evaluation on the antioxidant activity of a copper( ii ) complex based on lidocaine and ibuprofen amide-phenanthroline agents
Tabrizi, L., Dao, D. Q., & Vu, T. A. (2019) · RSC Advances · doi:10.1039/c8ra09763a · Cited by 71 · see citing works
Protective effects of polysaccharides from Psidium guajava leaves against oxidative stresses
Kim, S.-Y., Kim, E., Kim, Y.-S., Yu, S.-K., Choi, C., Lee, J., Kim, Y.-T., Nah, J., & Jeon, Y. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.05.111 · Cited by 69 · see citing works
“Thus, the mechanism of hydroxyl radical scavenging is related to transition of metal ions, and removal of hydroxyl radicals is important for protection of living systems [47].”
Background
Advances in green processing of seed oils using ultrasound‐assisted extraction: A review
Mushtaq, A., Roobab, U., Denoya, G. I., Inam‐Ur‐Raheem, M., Gullón, B., Lorenzo, J. M., Barba, F. J., Zeng, X., Wali, A., & Aadil, R. M. (2020) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.14740 · Cited by 68 · see citing works
Pharmacological and biomedical uses of extracts of pumpkin and its relatives and applications in the food industry: a review
Sharma, P., Kaur, G., Kehinde, B. A., Chhikara, N., Panghal, A., & Kaur, H. (2020) · International Journal of Vegetable Science · doi:10.1080/19315260.2019.1606130 · Cited by 66 · see citing works
Optimization of ultrasonic-assisted enzymatic extraction of polysaccharides from thick-shell mussel (Mytilus coruscus) and their antioxidant activities
Chen, W., Jia, Z., Zhu, J., Zou, Y., Huang, G., & Hong, Y. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.08.136 · Cited by 64 · see citing works
Holistic review of polysaccharides isolated from pumpkin: Preparation methods, structures and bioactivities
Li, F., Zhao, J., Wei, Y., Jiao, X., & Li, Q. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.10.037 · Cited by 63 · see citing works
Ultrasound enhanced laccase applications
Gonçalves, I., Silva, C., & Cavaco-Paulo, A. M. (2014) · Green Chemistry · doi:10.1039/c4gc02221a · Cited by 63 · see citing works
A new kinetic model of ultrasound-assisted extraction of polysaccharides from Chinese chive
Zhang, W.-N., Zhang, H., Lu, C.-Q., Luo, J., & Zha, X. (2016) · Food Chemistry · doi:10.1016/j.foodchem.2016.05.144 · Cited by 63 · see citing works
Extraction optimization, characterization and antioxidant activity of polysaccharide from Gentiana scabra bge
Cheng, Z., Zhang, Y., Song, H., Zhou, H., Zhong, F., Hu, H., & Feng, Y. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.08.059 · Cited by 61 · see citing works
Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids from Sophora flavescens Using Response Surface Methodology
Zhou, J. W., Zhang, L., Li, Q., Jin, W., Chen, W., Han, J., & Zhang, Y. (2018) · Molecules · doi:10.3390/molecules24010112 · Cited by 61 · see citing works
“It indicates that a liquid-to-material ratio that is too low or too high may not favor the movement of the plant cells and the flavonoids to the solvents under ultrasonic treatment [15].”
Background
Optimization of Ultrasonic-Assisted Enzymatic Extraction Conditions for Improving Total Phenolic Content, Antioxidant and Antitumor Activities In Vitro from Trapa quadrispinosa Roxb. Residues
Li, F., Mao, Y.-D., Wang, Y., Raza, A., Qiu, L., & Xu, X. (2017) · Molecules · doi:10.3390/molecules22030396 · Cited by 59 · see citing works
“Furthermore, high temperature could decrease number of cavitation bubbles and weaken the impact of cavity collapse on homogenized samples [38].”
Background
Research on structure and antioxidant activity of polysaccharides from Ginkgo biloba leaves
Wang, F., Ye, S., Ding, Y., Ma, Z., Zhao, Q., Zang, M., & Li, Y. (2021) · Journal of Molecular Structure · doi:10.1016/j.molstruc.2021.132185 · Cited by 56 · see citing works
Effects and mechanisms of ultrasound- and alkali-assisted enzymolysis on production of water-soluble yeast β-glucan
Zheng, Z., Huang, Q., Luo, X., Xiao, Y., Cai, W., & Ma, H. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.11.035 · Cited by 55 · see citing works
Structural characterization and antioxidant activity of polysaccharides extracted from Chinese yam by a cellulase-assisted method
Liu, X., Gu, L.-B., Zhang, G.-J., Liu, H., Zhang, Y., & Zhang, K.-P. (2022) · Process Biochemistry · doi:10.1016/j.procbio.2022.06.023 · Cited by 53 · see citing works
Ultrasonic-assisted enzymatic extraction of a water soluble polysaccharide from dragon fruit peel and its antioxidant activity
Qian, S., Fang, X., Dan, D., Diao, E., & Lu, Z. (2018) · RSC Advances · doi:10.1039/c8ra06449k · Cited by 52 · see citing works
Optimization of PEG-based extraction of polysaccharides from Dendrobium nobile Lindl. and bioactivity study
Zhang, Y., Wang, H., Wang, P., Ma, C., He, G., & Rahman, M. R. T. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.07.034 · Cited by 51 · see citing works
Soft liquefaction of the red seaweed Grateloupia turuturu Yamada by ultrasound-assisted enzymatic hydrolysis process
Guillard, C. L., Bergé, J.-P., Donnay‐Moreno, C., Bruzac, S., Ragon, J.-Y., Baron, R., Fleurence, J., & Dumay, J. (2016) · Journal of Applied Phycology · doi:10.1007/s10811-015-0788-x · Cited by 51 · see citing works
“This process is relatively new and has been mainly developed to convert vegetable biomass or waste into valuable sugars by enhancing the extraction and the hydrolysis of polysaccharides (Leaes et al. 2013; Lunelli et al. 2014; Liu et al. 2014; Wu et al. 2014).”
Background
Isolation of polysaccharides from Malva aegyptiaca and evaluation of their antioxidant and antibacterial properties
Fakhfakh, N., Abdelhedi, O., Jdir, H., Nasri, M., & Zouari, N. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.07.105 · Cited by 51 · see citing works
Evaluation of the Chemical Composition and Antioxidant Activity of Mulberry (Morus alba L.) Fruits from Different Varieties in China
Chen, T., Shuang, F.-F., Fu, Q.-Y., Ju, Y.-X., Zong, C.-M., Zhao, W.-G., Zhang, D., Yao, X.-H., & Cao, F. (2022) · Molecules · doi:10.3390/molecules27092688 · Cited by 49 · see citing works
“’s method [38], with slight modifications.”
Anti-glycation effect of Ecklonia cava polysaccharides extracted by combined ultrasound and enzyme-assisted extraction
Park, J. J., & Lee, W. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.03.118 · Cited by 49 · see citing works
Recent Advances in the Application of Enzyme Processing Assisted by Ultrasound in Agri-Foods: A Review
Córdova, A., Henríquez, P., Núñez, H., Rico-Rodríguez, F., Guerrero, C., Astudillo‐Castro, C., & Illanes, A. (2022) · Catalysts · doi:10.3390/catal12010107 · Cited by 47 · see citing works
Total Monomeric Anthocyanin, Total Phenolic Content and Antioxidant Activity of Extracts from Eggplant ( S olanum Melongena L .) Peel Using Ultrasonic Treatments
Dranca, F., & Oroian, M. A. (2015) · Journal of Food Process Engineering · doi:10.1111/jfpe.12312 · Cited by 46 · see citing works
Bioactivities of Nizamuddinia zanardinii sulfated polysaccharides extracted by enzyme, ultrasound and enzyme-ultrasound methods
Alboofetileh, M., Rezaei, M., Tabarsa, M., & You, S. (2019) · Journal of Food Science and Technology · doi:10.1007/s13197-019-03584-1 · Cited by 46 · see citing works
Ultrasound assisted intensified enzymatic extraction of total phenolic compounds from pomegranate peels
Patil, N., Yadav, P., & Gogate, P. R. (2024) · Separation and Purification Technology · doi:10.1016/j.seppur.2024.127967 · Cited by 45 · see citing works
Physicochemical Properties and Antioxidant Activity of Pumpkin Polysaccharide (Cucurbita moschata Duchesne ex Poiret) Modified by Subcritical Water
Yu, G., Zhao, J., Wei, Y., Huang, L., Li, F., Zhang, Y., & Li, Q. (2021) · Foods · doi:10.3390/foods10010197 · Cited by 45 · see citing works
Nonconventional Hydrocolloids’ Technological and Functional Potential for Food Applications
Lopez, S. V. M., Zuluaga‐Domínguez, C. M., Fernández-Trujillo, J. P., & Hernández-Gómez, M. S. (2022) · Foods · doi:10.3390/foods11030401 · Cited by 46 · see citing works
Optimized Conditions for Flavonoid Extraction from Pomelo Peel Byproducts under Enzyme- and Ultrasound-Assisted Extraction Using Response Surface Methodology
Anh, M. N. T., Hung, P. V., & Phi, N. T. L. (2021) · Journal of Food Quality · doi:10.1155/2021/6666381 · Cited by 46 · see citing works
“[20] found that the higher recovery of polysaccharides was obtained when applying ultrasound to enzyme pretreated samples or simultaneous enzyme and ultrasound extraction compared to conventional extraction, EAE or UAE alone.”
Result
Ultrasound-assisted three phase partitioning for simultaneous extraction of oil, protein and polysaccharide from pumpkin seeds
Wang, H., Chen, K., Cheng, J., Jiang, L., Yu, D., Dai, Y., & Wang, L. (2021) · LWT · doi:10.1016/j.lwt.2021.112200 · Cited by 43 · see citing works
Simultaneous optimization of ultrasonic-assisted extraction of Danshen for maximal tanshinone IIA and salvianolic acid B yields and antioxidant activity: A comparative study of the response surface methodology and artificial neural network
Yu, Z., Zhang, Y., Zhao, X., Yu, L., Chen, X., Wan, H., He, Y. T., & Jin, W. (2020) · Industrial Crops and Products · doi:10.1016/j.indcrop.2020.113199 · Cited by 41 · see citing works
Development and optimization of pluronic® F127 and HPMC based thermosensitive gel for the skin delivery of metoprolol succinate
Parhi, R. (2016) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2016.09.004 · Cited by 41 · see citing works
Optimization of fucoidan recovery by ultrasound-assisted enzymatic extraction from South African kelp, Ecklonia maxima
Mapholi, Z., & Goosen, N. J. (2023) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2023.106710 · Cited by 42 · see citing works
“Similarly, the increase in temperature leads to an increased enzymatic hydrolysis rate which could potentially explain the increase in the yields of total dissolved solids and total carbohydrates [34,47].”
Background
Studies on cellulase-ultrasonic assisted extraction technology for flavonoids from Illicium verum residues
Huang, D., Zhou, X., Si, J., Gong, X., & Wang, S. (2016) · Chemistry Central Journal · doi:10.1186/s13065-016-0202-z · Cited by 40 · see citing works
“On the other hand, enzymeassisted extraction uses enzyme preparations either alone or in mixtures that catalyze hydrolysis of the cytoderm and glycoproteins, and enhance the release of bioactive substances by disrupting plant cells [14].”
Background
Extraction, purification and antioxidant activity of polysaccharide from cold pressed oil cake of ‘Tengjiao’ seed
Xu, D., Wang, C., Zhuo, Z., Ye, M., & Biao, P. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.06.207 · Cited by 39 · see citing works
Ultrasound-assisted enzymatic extraction, structural characterization, and anticancer activity of polysaccharides from Rosa roxburghii Tratt fruit
Jiao, X., Zhang, M., Zhang, M., Hao, L., & Wu, C. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.127926 · Cited by 38 · see citing works
Ultrasound-assisted enzymatic extraction of polysaccharides from Paulownia flowers: process optimization, structural characterization, antioxidant and hypoglycemic activities
Lv, T., Liu, X., Tao, J., Zhang, Y., Xie, Q., Meng, X., & Liu, X. (2024) · Microchemical Journal · doi:10.1016/j.microc.2024.109940 · Cited by 38 · see citing works
Enhancing Phenolic Compound Recovery from Grape Pomace Residue: Synergistic Approach of Ultrasound- and Enzyme-Assisted Extraction
Stanek, N., Zarębska, M., Wasilewski, T., Hordyjewicz‐Baran, Z., Krzyszowska, A., Gębura, K., & Tomaka, M. (2025) · ACS Omega · doi:10.1021/acsomega.5c01321 · Cited by 38 · see citing works
Study on the Optimization, Extraction Kinetics and Thermodynamics of the Ultrasound-Assisted Enzymatic Extraction of Tremella fuciformis Polysaccharides
Hou, F., Song, S., Yang, S., Wang, Y., Jia, F., & Wang, W. (2024) · Foods · doi:10.3390/foods13091408 · Cited by 36 · see citing works
“…one hand, may not have been conducive to the movement of plant cells to the active site of the enzyme, and on the other hand, it was too excessive compared with the fixed liquid–material ratio, and it accordingly resulted in a reduction in enzymatic hydrolysis and lowered the extraction yield [21].”
Background
Enzyme-assisted extraction of plant material – New functional aspects of the process on an example of Medicago sativa L.
Rafińska, K., Wrona, O., Krakowska‐Sieprawska, A., Walczak‐Skierska, J., Kiełbasa, A., Rafiński, Z., Pomastowski, P., Kolankowski, M., & Buszewski, B. (2022) · Industrial Crops and Products · doi:10.1016/j.indcrop.2022.115424 · Cited by 35 · see citing works
Variation in total anthocyanin, phenolic contents, antioxidant enzyme and antioxidant capacity among different mulberry ( Morus sp.) cultivars in China
Chen, H., Chen, J., Yang, H., Chen, W., Gao, H., & Lu, W. (2016) · Scientia Horticulturae · doi:10.1016/j.scienta.2016.10.036 · Cited by 35 · see citing works
Preparation and antioxidant activities of polysaccharides obtained from abalone viscera by combination of enzymolysis and multiple separation methods
Guo, S., Jiao, W., He, C., Wei, H., Ma, Y., & Xiong, H. (2020) · Journal of Food Science · doi:10.1111/1750-3841.15520 · Cited by 35 · see citing works
Extraction, Structural Characterization, Biological Functions, and Application of Rice Bran Polysaccharides: A Review
Chen, B., Qiao, Y., Wang, X. D., Zhang, Y., & Fu, L. (2023) · Foods · doi:10.3390/foods12030639 · Cited by 35 · see citing works
“[14] xperimentally concluded that compared with polysaccharides obtained using the hydrother al meth d (Mw = 1.”
BackgroundMethodResultInfluential
Formulation optimization and characterization of transdermal film of simvastatin by response surface methodology
Parhi, R., & Suresh, P. (2015) · Materials Science and Engineering C · doi:10.1016/j.msec.2015.08.056 · Cited by 33 · see citing works
Extraction and characterization of polysaccharides from Schisandra sphenanthera fruit by Lactobacillus plantarum CICC 23121-assisted fermentation
Wang, Q., Hao, L., Zhang, A., Zhao, H., & Zhang, B. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.129135 · Cited by 32 · see citing works
Optimization of R-Phycoerythrin Extraction by Ultrasound-Assisted Enzymatic Hydrolysis: A Comprehensive Study on the Wet Seaweed Grateloupia turuturu
Guillard, C. L., Bergé, J.-P., Donnay‐Moreno, C., Cornet, J., Ragon, J.-Y., Fleurence, J., & Dumay, J. (2023) · Marine Drugs · doi:10.3390/md21040213 · Cited by 29 · see citing works
“This result is not surprising, knowing that the enzymes used here are dedicated to the hydrolysis of polysaccharides (glycosidases) and ultrasound have already been shown to intensify the enzymatic hydrolysis of various biomass for the release of carbohydrates and polysaccharides [40,47,48,50,51,54,59].”
Background
Low-Frequency, High-Power Ultrasound-Assisted Food Component Extraction
Cravotto, G., & Binello, A. E. (2016) · Elsevier eBooks · doi:10.1016/b978-0-08-100294-0.00001-8 · Cited by 29 · see citing works
Recovery of carotenoids as bioactive compounds from peach pomace by an eco-friendly ultrasound-assisted enzymatic extraction
Han, K. N., Meral, H., & Demirdöven, A. (2024) · Journal of Food Science and Technology · doi:10.1007/s13197-024-06001-4 · Cited by 28 · see citing works
“This indicates that there is a high degree of correlation between the real and predicted data in the created regression model (Wu et al. 2014).”
Enzymolysis-based ultrasound extraction and antioxidant activities of polyprenol lipids from Ginkgo biloba leaves
Zhang, C., Wang, C., Tao, R., & Fang, C. (2015) · Process Biochemistry · doi:10.1016/j.procbio.2015.12.008 · Cited by 28 · see citing works
Efficient extraction, physiochemical, rheological properties, and antioxidant activities of polysaccharides from Armeniaca vulgaris Lam
Sun, W., Labreche, F., Kou, X., Wu, C., Fan, G., Li, T., Suo, A., & Wu, Z. (2022) · Process Biochemistry · doi:10.1016/j.procbio.2022.04.032 · Cited by 27 · see citing works
A new kinetic model of ultrasound-assisted pretreatment on rice protein
Li, S., Ma, H., Guo, Y., Oladejo, A. O., Yang, X., Liang, Q., & Duan, Y. (2017) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2017.07.010 · Cited by 27 · see citing works
“Weibell’s model is widely applied in the extraction process of active substance from plant materials widely because it is simplicity and applicability [33].”
Background
Purification, characterization, antioxidant, and antitumor activity of polysaccharides isolated from silkworm cordyceps
Shi, K., Yang, G., He, L., Yang, B., Li, Q., & Yi, S. (2020) · Journal of Food Biochemistry · doi:10.1111/jfbc.13482 · Cited by 26 · see citing works
Enzyme Assisted Extraction, Purification and Structure Analysis of the Polysaccharides from Naked Pumpkin Seeds
Wang, L., Liu, F., Li, T., Liu, D., Xu, Y., & Yang, Y. (2018) · Applied Sciences · doi:10.3390/app8101866 · Cited by 26 · see citing works
“This result may be due to low enzyme activities at higher pH values [22].”
Background
Pumpkin Skin Polysaccharide–Zn(II) Complex: Preparation, Characterization, and Suppression of Inflammation in Zebrafish
Shujun, D. O. N. G., Zhang, B., Ma, Y., Chang, H., Zheng, Z., & Zhao, X. (2022) · Foods · doi:10.3390/foods11172610 · Cited by 26 · see citing works
“The thick layer of pumpkin skin is usually treated as a waste of processing, which accounts for about 10-40% of the total quantity of the pumpkin, resulting in a low comprehensive utilization rate and environmental pollution [15].”
Background
Extraction, partial characterization and bioactivity of polysaccharides from Senecio scandens Buch.-Ham
Yu, J., Hu, M., Wang, Y., Zhang, Q., Xu, W., & Su, W. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.12.119 · Cited by 26 · see citing works
Design and optimization of film-forming gel of etoricoxib using research surface methodology
Parhi, R., & Goli, V. V. N. (2019) · Drug Delivery and Translational Research · doi:10.1007/s13346-019-00695-2 · Cited by 25 · see citing works
“However, the data obtained at 24 h of permeation study was considered and displayed as Q24.”
BackgroundMethodInfluential
Positive effects of ultrasound pretreatment on the bioaccessibility and cellular uptake of bioactive compounds from broccoli: Effect on cell wall, cellular matrix and digesta
Zhang, Z., Nie, M., Xiao, Y., Zhu, L., Gao, R., Zhou, C., Li, D., Xu, Y., & Dai, Z. (2021) · LWT · doi:10.1016/j.lwt.2021.112052 · Cited by 25 · see citing works
Optimization of enzymatic extraction, characterization and bioactivities of Se-polysaccharides from Se-enriched Lentinus edodes
Gu, H., Liang, L., Zhu, X. P., Jiang, X., Du, M., & Wang, Z. (2022) · Food Bioscience · doi:10.1016/j.fbio.2022.102346 · Cited by 25 · see citing works
Fortification of γ-aminobutyric acid and bioactive compounds in Cucurbita moschata by novel two-step fermentation using Bacillus subtilis and Lactobacillus plantarum
Park, E. J., Garcia, C. V., Youn, S.-J., Park, C.-D., & Lee, S. (2018) · LWT · doi:10.1016/j.lwt.2018.07.065 · Cited by 25 · see citing works
Combining high-power ultrasound and enological enzymes during winemaking to improve the chromatic characteristics of red wine
Pérez‐Porras, P., Bautista‐Ortín, A. B., Jurado, R., & Gómez‐Plaza, E. (2021) · LWT · doi:10.1016/j.lwt.2021.113032 · Cited by 24 · see citing works
Effect of ultrasound- and pulsed electric field-assisted enzymatic treatment on the recovery and quality of sunflower oil
Moradi, N., & Rahimi, M. (2018) · Separation Science and Technology · doi:10.1080/01496395.2018.1522344 · Cited by 23 · see citing works
Ultrasound-assisted extraction and antioxidant activity of polysaccharides from Tenebrio molitor
Yang, S., Li, X., & Zhang, H. (2024) · Scientific Reports · doi:10.1038/s41598-024-79482-0 · Cited by 22 · see citing works
Extraction, Separation, Purification, Chemical Characterizations, and Biological Activities of Polysaccharides from Chinese Herbal Medicine: A Review
Zhang, P., Tan, J., Wang, W., Zhang, J., Gong, H., & Xue, H. (2022) · Starch - Stärke · doi:10.1002/star.202200114 · Cited by 22 · see citing works
Pumpkin Polysaccharide Extracted by Subcritical Water: Physicochemical Characterization and Anti‐Diabetic Effects in T2DM Rats
Ti, Y., Wang, W., Wang, X., Ban, Y., Wang, P., Zhang, Y. L., & Song, Z. (2022) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.202200160 · Cited by 22 · see citing works
Extraction and characterization of bioactive compounds from eggplant peel using ultrasound – assisted extraction
Horincar, G., de Jos", D., Enachi, E., Stănciuc, N., & Gabriela Râpeanu, B. (2019) · The Annals of the University Dunarea de Jos of Galati Fascicle VI – Food Technology · doi:10.35219/foodtechnology.2019.1.03 · Cited by 22 · see citing works
“…compounds using an ultrasound-assisted process was applied to several types of compounds such as polysaccharides from pumpkin and black currant (Wu et al., 2014, Xu et al., 2015), grape amino acids (Carrera et al., 2015), black tea polyphenols (Both et al., 2014) and papaya seed oil (Samaram…”
Method
RSM-Based Design and Optimization of Transdermal Film of Ondansetron HCl
Parhi, R., & Panchamukhi, T. (2019) · Journal of Pharmaceutical Innovation · doi:10.1007/s12247-019-09373-9 · Cited by 22 · see citing works
Recent advances in the extraction, purification, and antioxidant activities of fruit polysaccharides: A review
Mohanta, B., Sen, D. J., Mahanti, B., & Nayak, A. K. (2023) · Food Hydrocolloids for Health · doi:10.1016/j.fhfh.2023.100163 · Cited by 21 · see citing works

Extraction of Polysaccharides from Pumpkin by Ultrasonic Method and it’s Primary Antioxidant Activities Research
Jie, S., Yin, G. Y., Zhang, H., Chen, L. Y., & Hu, C. (2011) · Advanced materials research · doi:10.4028/www.scientific.net/amr.183-185.1970 · Cited by 2 · see citing works
Modeling and optimization of ultrasound-assisted extraction of polysaccharide from Cucurbita moschata
Maran, J. P., Mekala, V., & Manikandan, S. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2012.11.086 · Cited by 195 · see citing works
Ultrasonic-assisted enzymatic extraction of polysaccharide from Corbicula fluminea: Characterization and antioxidant activity
Liao, N., Zhong, J., Ye, X., Lu, S., Wang, W., Zhang, R., Xu, J. F., Chen, S., & Liu, D. (2014) · LWT · doi:10.1016/j.lwt.2014.10.009 · Cited by 122 · see citing works
Ultrasonic-assisted extraction and antioxidant activity of polysaccharides from Eucommia ulmoides leaf
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Authors: Wu, H., Zhu, J., Diao, W., & Wang, C.
Affiliations: Qingdao Agricultural University (CN)
Corresponding author: Wang, Chengrong
ORCID: Wu, Hao
Zhu, Junxiang
Wang, Chengrong
Venue: Carbohydrate Polymers
Publisher: Elsevier BV
Published: 2014-11
Volume: 113
Pages: 314-324
ISSN: 0144-8617 (ISSN-L)
DOI: 10.1016/j.carbpol.2014.07.025
OpenAlex: W2072936136
Cited by: 169
References: 60
Citations by year: 2026: 8 · 2025: 6 · 2024: 22 · 2023: 12 · 2022: 21 · 2021: 22 · 2020: 13 · 2019: 15 · 2018: 13 · 2017: 9 · 2016: 15 · 2015: 11 · 2014: 2
FWCI: 17.67
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Carbohydrate Polymers (publishedVersion)
PubMed (publishedVersion)
Funders: Modern Vegetable Industry Technology System of Shandong Province (SDAIT-02-022-12)
Shandong province Higher Educational Science and Technology Program (J14LE11)
Talents of Highlevel Scientific Research Foundation of Qingdao Agriculture University (631207)
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
Keywords: Cucurbita moschata, pumpkin polysaccharide, ultrasound-assisted enzymatic extraction, antioxidant activity, extraction optimization, mass transfer kinetics, enzymatic extraction, DPPH radical scavenging, reducing power, natural antioxidants
MeSH terms: Antioxidants, Polysaccharides, Sound, Free Radical Scavengers, Cucurbita

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Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata)

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