The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Exopolysaccharides of Lactobacillus reuteri: Their Influence on Adherence of E. Coli to Epithelial Cells and Inflammatory Response
Abstract
The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 Biocenol™ (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were d-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1→4) and (1→6) glycosidic linkages, increased IL-1β gene expression (0.1mg/mL; P<0.05), while EPS-L26, more branched polysaccharide with (1→3) and (1→6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-κB, TNF-α and IL-6 mRNA (P<0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P<0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P<0.01).
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“High-performance, anion-exchange chromatography coupled with pulsed amperometric detection [8,27,35,53] or gas chromatography (GC) [32,43] are commonly utilized to analyze the monosaccharide composition of EPS.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [32]
Citations
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Ale, E. C., Puntillo, M. A., Rojas, M. F., & Binetti, A. G. (2022) · doi:10.1002/9781119769620.ch15
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Shaposhnikov, L. A., Tishkov, V. I., & POMETUN, A. A. (2024) · Biochemistry (Moscow) · doi:10.1134/s0006297924140050
“In addition, EPS synthesized by L. reuteri is able to inhibit adhesion of E. coli to pig epithelial cells in vitro [81].”
Wang, Y., Yue, Y., Wang, L., Li, J., Duan, S., Li, B., & Yu, D. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.142612
RetractedPatadiya, M., Suthar, Y., & Gupte, S. A. (2025) · Microbiology and Biotechnology Letters · doi:10.48022/mbl.2503.03008
Çetin, O., Darilmaz, D. Ö., & YILDIZ, G. G. (2025) · International Journal of Food Science & Technology · doi:10.1093/ijfood/vvaf225
Butorac, K., Novak, J., Bellich, B., Terán, L. C., Banić, M., Leboš Pavunc, A., Zjalić, S., Cescutti, P., Šušković, J., & Kos, B. (2021) · Figshare · doi:10.6084/m9.figshare.14445034
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Pramudito, T. E., Klostermann, C. E., Smid, E. J., & Schols, H. A. (2026) · Applied Food Research · doi:10.1016/j.afres.2026.102204
Zou, Y., Xu, X., Zhang, S., Chen, X., Zhang, W., Duan, C., Liu, R., Luo, W., & Zhou, Y. (2026) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2026.153612
Zhong, W., He, H., Hu, K., Huang, K., Jiang, B., Liu, C., Huang, Y., 苏友禄, & Li, W. (2026) · Brazilian Journal of Microbiology · doi:10.1007/s42770-026-02085-6
“Extracellular exo-polysaccharides produced by L. reuteri strains DSM and L26 have been shown to inhibit the adhesion of enterotoxigenic Escherichia coli and simultaneously induced the expression of pro-inflammatory cytokines IL-1 and IL-6 [13] .”
DİKİCİ, A., Darilmaz, D. Ö., İnanan, T., Yesildal, T. K., & Yüksekdağ, Z. (2026) · Food Bioscience · doi:10.1016/j.fbio.2026.110170
Farah, H. S., Padmapriya, G., Sahoo, S., Srivastav, S. K., Khdir, M. M., Rani, A., Axmedova, F., & Бекзод, Б. Э. (2026) · Folia Microbiologica · doi:10.1007/s12223-026-01608-w
Metadata
- Authors
- Kšonžeková, P., Bystrický, P., Vlčková, S., Pätoprstý, V., Pulzová, L., Mudroňová, D., Kubašková, T., Csank, T., & Tkáčiková, Ľ.
- Affiliations
- University of Veterinary Medicine in Košice (SK)
- Institute of Chemistry of the Slovak Academy of Sciences (SK)
- Corresponding author
- Tkáčiková, Ľudmila
- ORCID
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 141
- Pages
- 10-19
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W2207148956
- Cited by
- 122
- References
- 38
- Citations by year
- 2026: 9 · 2025: 16 · 2024: 22 · 2023: 14 · 2022: 18 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 12 · 2017: 9 · 2016: 2
- FWCI
- 10.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- Funders
- VEGA (1/0834/12)
- Probiotech (26220220152)
- European Fund (OAE010101)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- Lactobacillus reuteri, exopolysaccharides, enterotoxigenic Escherichia coli, bacterial adhesion, IPEC-1 cells, intestinal epithelial cells, inflammatory cytokines, IL-1β, interleukin-6, TNF-α, NF-κB, glycosidic linkage, polysaccharide molecular weight, prebiotics
- MeSH terms
- Animals, Bacterial Adhesion, Cell Line, Epithelial Cells, Glucans, Polysaccharides, Bacterial, RNA, Messenger, Swine, Tumor Necrosis Factor-alpha, Interleukin-6, Up-Regulation, NF-kappa B, Limosilactobacillus reuteri, Interleukin-1beta, Enterotoxigenic Escherichia coli
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