CoChat
Sign Up
Cited by 476Cellular transport and secretionCaveolin-1 and cellular processesMicrotubule and mitosis dynamics

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Coupling between Clathrin-Coated-Pit Invagination, Cortactin Recruitment, and Membrane Scission Observed in Live Cells

Open access
Merrifield, Christien J., Perrais, David, Zenisek, David·2005·Cell
DOI 10.1016/j.cell.2005.03.015
Open this paper in CoChat — free accountOpen PDF

Abstract

During clathrin-mediated endocytosis, membrane scission marks the isolation of a cargo-laden clathrin-coated pit (CCP) from the cell exterior. Here we used live-cell imaging of a pH-sensitive cargo to visualize the formation of clathrin-coated vesicles (CCVs) at single CCPs with a time resolution of seconds. We show that CCPs are highly dynamic and can produce multiple vesicles in succession. Using alternating evanescent field and epifluorescence illumination, we show that CCP invagination and scission are tightly coupled, with scission coinciding with maximal displacement of CCPs from the plasma membrane and with peak recruitment of cortactin-DsRed, a dynamin and F-actin binding protein. Finally, perturbing actin polymerization with latrunculin-B drastically reduces the efficiency of membrane scission and affects many aspects of CCP dynamics. We propose that CCP invagination, actin polymerization, and CCV formation are highly coordinated for efficient endocytosis.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.cell.2005.03.015

Cites

0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 44

  1. Blanpied, 2002, Dynamics and regulation of clathrin coats at specialized endocytic zones of dendrites and spines, Neuron, 36, 435, 10.1016/S0896-6273(02)00979-0 · DOI
  2. Bretscher, 1984, Endocytosis: relation to capping and cell locomotion, Science, 224, 681, 10.1126/science.6719108 · DOI
  3. Cao, 2003, Cortactin is a component of clathrin-coated pits and participates in receptor-mediated endocytosis, Mol. Cell. Biol., 23, 2162, 10.1128/MCB.23.6.2162-2170.2003 · DOI
  4. Daly, 2004, Cortactin signalling and dynamic actin networks, Biochem. J., 382, 13, 10.1042/BJ20040737 · DOI
  5. Ehrlich, 2004, Endocytosis by random initiation and stabilization of clathrin-coated pits, Cell, 118, 591, 10.1016/j.cell.2004.08.017 · DOI · Paper page
  6. Engqvist-Goldstein, 2001, The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro, J. Cell Biol., 154, 1209, 10.1083/jcb.200106089 · DOI
  7. Farsad, 2003, Mechanisms of membrane deformation, Curr. Opin. Cell Biol., 15, 372, 10.1016/S0955-0674(03)00073-5 · DOI
  8. Fujimoto, 2000, Actin assembly plays a variable, but not obligatory role in receptor-mediated endocytosis in mammalian cells, Traffic, 1, 161, 10.1034/j.1600-0854.2000.010208.x · DOI
  9. Gaidarov, 1999, Spatial control of coated-pit dynamics in living cells, Nat. Cell Biol., 1, 1, 10.1038/8971 · DOI
  10. Giardini, 2003, Compression forces generated by actin comet tails on lipid vesicles, Proc. Natl. Acad. Sci. USA, 100, 6493, 10.1073/pnas.1031670100 · DOI
  11. Heuser, 1980, Three-dimensional visualization of coated vesicle formation in fibroblasts, J. Cell Biol., 84, 560, 10.1083/jcb.84.3.560 · DOI
  12. Heuser, 1989, Effects of cytoplasmic acidification on clathrin lattice morphology, J. Cell Biol., 108, 401, 10.1083/jcb.108.2.401 · DOI
  13. Heuser, 1989, Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation, J. Cell Biol., 108, 389, 10.1083/jcb.108.2.389 · DOI
  14. Higgins, 2002, Snap-shots of clathrin-mediated endocytosis, Trends Biochem. Sci., 27, 257, 10.1016/S0968-0004(02)02089-3 · DOI
  15. Iversen, 2003, Clathrin-coated pits with long, dynamin-wrapped necks upon expression of a clathrin antisense RNA, Proc. Natl. Acad. Sci. USA, 100, 5175, 10.1073/pnas.0534231100 · DOI
  16. Kaksonen, 2000, Association of cortactin with dynamic actin in lamellipodia and on endosomal vesicles, J. Cell Sci., 113, 4421, 10.1242/jcs.113.24.4421 · DOI
  17. Kaksonen, 2003, A pathway for association of receptors, adaptors, and actin during endocytic internalization, Cell, 115, 475, 10.1016/S0092-8674(03)00883-3 · DOI · Paper page
  18. Kessels, 2002, Syndapins integrate N-WASP in receptor-mediated endocytosis, EMBO J., 21, 6083, 10.1093/emboj/cdf604 · DOI
  19. Keyel, 2004, Endocytic adaptor molecules reveal an endosomal population of clathrin by total internal reflection fluorescence microscopy, J. Biol. Chem., 279, 13190, 10.1074/jbc.M312717200 · DOI
  20. McNiven, 2000, Regulated interaction between dynamin and the actin-binding protein cortactin modulate cell shape, J. Cell Biol., 151, 187, 10.1083/jcb.151.1.187 · DOI
  21. Merrifield, 2004, Seeing is believing: imaging actin dynamics at single sites of endocytosis, Trends Cell Biol., 14, 352, 10.1016/j.tcb.2004.05.008 · DOI
  22. Merrifield, 2002, Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits, Nat. Cell Biol., 4, 691, 10.1038/ncb837 · DOI · Paper page
  23. Merrifield, 2004, Neural Wiskott Aldrich Syndrome Protein (N-WASP) and the Arp2/3 complex are recruited to sites of clathrin-mediated endocytosis in cultured fibroblasts, Eur. J. Cell Biol., 83, 13, 10.1078/0171-9335-00356 · DOI
  24. Miesenbock, 1998, Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins, Nature, 394, 192, 10.1038/28190 · DOI
  25. Mise-Omata, 2003, Mammalian actin binding protein 1 is essential for endocytosis but not lamellipodia formation: functional analysis by RNA interference, Biochem. Biophys. Res. Commun., 301, 704, 10.1016/S0006-291X(02)02972-8 · DOI
  26. Mukherjee, 1997, Endocytosis, Physiol. Rev., 77, 759, 10.1152/physrev.1997.77.3.759 · DOI
  27. Perrais, 2004, Recapture after exocytosis causes differential retention of protein in granules of bovine chromaffin cells, J. Physiol., 560, 413, 10.1113/jphysiol.2004.064410 · DOI
  28. Praefcke, 2004, The dynamin superfamily: universal membrane tubulation and fission molecules?, Nat. Rev. Mol. Cell Biol., 5, 133, 10.1038/nrm1313 · DOI
  29. Qualmann, 2000, Molecular links between endocytosis and the actin cytoskeleton, J. Cell Biol., 150, F111, 10.1083/jcb.150.5.F111 · DOI
  30. Rappoport, 2003, The AP-2 complex is excluded from the dynamic population of plasma membrane-associated clathrin, J. Biol. Chem., 278, 47357, 10.1074/jbc.C300390200 · DOI
  31. Rappoport, 2004, Understanding living clathrin-coated pits, Traffic, 5, 327, 10.1111/j.1398-9219.2004.00187.x · DOI
  32. Sandvig, 1987, Acidification of the cytosol inhibits endocytosis from coated pits, J. Cell Biol., 105, 679, 10.1083/jcb.105.2.679 · DOI
  33. Sankaranarayanan, 2000, Real-time measurements of vesicle-SNARE recycling in synapses of the central nervous system, Nat. Cell Biol., 2, 197, 10.1038/35008615 · DOI
  34. Santini, 2002, A glimpse of coated vesicle creation? Well almost!, Nat. Cell Biol., 4, E230, 10.1038/ncb1002-e230 · DOI
  35. Schafer, 2002, Dynamin2 and cortactin regulate actin assembly and filament organization, Curr. Biol., 12, 1852, 10.1016/S0960-9822(02)01228-9 · DOI
  36. Schmid, 1991, Stage-specific assays for coated pit formation and coated vesicle budding in vitro, J. Cell Biol., 114, 869, 10.1083/jcb.114.5.869 · DOI
  37. Steyer, 2001, A real-time view of life within 100 nm of the plasma membrane, Nat. Rev. Mol. Cell Biol., 2, 268, 10.1038/35067069 · DOI
  38. Toomre, 2000, Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy, J. Cell Biol., 149, 33, 10.1083/jcb.149.1.33 · DOI
  39. Upadhyaya, 2003, Probing polymerization forces by using actin-propelled lipid vesicles, Proc. Natl. Acad. Sci. USA, 100, 4521, 10.1073/pnas.0837027100 · DOI
  40. Weed, 2000, Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex, J. Cell Biol., 151, 29, 10.1083/jcb.151.1.29 · DOI
  41. Willingham, 1983, Formation of receptosomes from plasma membrane coated pits during endocytosis: analysis by serial sections with improved membrane labeling and preservation techniques, Proc. Natl. Acad. Sci. USA, 80, 5617, 10.1073/pnas.80.18.5617 · DOI
  42. Willingham, 1981, Receptor-mediated endocytosis in cultured fibroblasts: cryptic coated pits and the formation of receptosomes, J. Histochem. Cytochem., 29, 1003, 10.1177/29.9.6169759 · DOI
  43. Yamashiro, 1984, Acidification of endocytic compartments and the intracellular pathways of ligands and receptors, J. Cell. Biochem., 26, 231, 10.1002/jcb.240260404 · DOI
  44. Yarar, 2004, A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosis, Mol. Biol. Cell, 16, 964, 10.1091/mbc.E04-09-0774 · DOI · Paper page

Cited by

Sorted by citations

See all 476

Metadata

Authors
Merrifield, C. J., Perrais, D., & Zenisek, D.
Affiliations
  • MRC Laboratory of Molecular Biology (GB)
  • Centre National de la Recherche Scientifique (FR)
  • Yale University (US)
Corresponding author
Merrifield, Christien J.
ORCID
Venue
Cell
Publisher
Elsevier BV
Published
2005-05
Volume
121
Issue
4
Pages
593-606
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2005.03.015
OpenAlex
W2001532503
Cited by
476
References
0
Citations by year
2026: 3 · 2025: 6 · 2024: 6 · 2023: 14 · 2022: 15 · 2021: 10 · 2020: 9 · 2019: 11 · 2018: 18 · 2017: 17 · 2016: 13 · 2015: 21 · 2014: 36 · 2013: 12 · 2012: 34
FWCI
16.53
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
clathrin-mediated endocytosis, membrane fission, clathrin-coated vesicles, cortactin, actin polymerization, latrunculin B, vesicle formation, dynamin, F-actin
MeSH terms
Actins, Animals, Cell Membrane, Coated Pits, Cell-Membrane, Endocytosis, Microfilament Proteins, Microscopy, Fluorescence, Polymers, Thiazoles, Microscopy, Video, Bridged Bicyclo Compounds, Heterocyclic, Clathrin-Coated Vesicles, Swiss 3T3 Cells, Cortactin, Mice, Thiazolidines

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review