Cited by 726Cellular transport and secretionFungal and yeast genetics researchMicrotubule and mitosis dynamics

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A Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery

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Kaksonen, Marko, Toret, Christopher P., Drubin, David G.·2005·Cell

DOI 10.1016/j.cell.2005.09.024

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Abstract

Endocytosis depends on an extensive network of interacting proteins that execute a series of distinct subprocesses. Previously, we used live-cell imaging of six budding-yeast proteins to define a pathway for association of receptors, adaptors, and actin during endocytic internalization. Here, we analyzed the effects of 61 deletion mutants on the dynamics of this pathway, revealing functions for 15 proteins, and we analyzed the dynamics of 8 of these proteins. Our studies provide evidence for four protein modules that cooperate to drive coat formation, membrane invagination, actin-meshwork assembly, and vesicle scission during clathrin/actin-mediated endocytosis. We found that clathrin facilitates the initiation of endocytic-site assembly but is not needed for membrane invagination or vesicle formation. Finally, we present evidence that the actin-meshwork assembly that drives membrane invagination is nucleated proximally to the plasma membrane, opposite to the orientation observed for previously studied actin-assembly-driven motility processes.

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Actin Assembly and Endocytosis: From Yeast to Mammals
Engqvist-Goldstein, Å. E. Y., & Drubin, D. G. (2003) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.19.111401.093127 · Cited by 572
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Coupling between Clathrin-Coated-Pit Invagination, Cortactin Recruitment, and Membrane Scission Observed in Live Cells
Merrifield, C. J., Perrais, D., & Zenisek, D. (2005) · Cell · doi:10.1016/j.cell.2005.03.015 · Cited by 476 · cited 2×
“The Rvs proteins arrive after the actin marker Abp1p has started to accumulate but reach their peak fluorescence intensity together with Abp1p (Figures 6B and 6C), consistent with the timing of scission during mammalian endocytosis (Merrifield et al., 2005).”

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Correction for Analysis of yeast endocytic site formation and maturation through a regulatory transition point
Carroll, S., Stimpson, H. E. M., Weinberg, J., Toret, C. P., Sun, Y.-D., & Drubin, D. (2012) · Molecular Biology of the Cell · doi:10.1091/mboc.23.16.3275
“Ede1p was hypothesized to be involved in endocytic site establishment because it affects the formation of productive endocytic sites (Kaksonen et al., 2005; Stimpson et al., 2009).”
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Rôle de la clathrine dans la formation des lamellipodes
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アンフィファイジンとN-WASPのダイナミックな相互作用は，アクチン重合を制御する
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“(Kaksonen et al., 2005).”
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“Other proteins are important for endocytic internalization additionally, such as fimbrin (Sac6p), calmodulin (Cmd1p), Rvs161p, Rvs167p, Cap1p, Cap2p and actin related protein Arp2p (Kübler and Riezman, 1993; Kübler et al, 1994; Kaksonen et al, 2005; Moreau et al, 1997).”
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Authors: Kaksonen, M., Toret, C. P., & Drubin, D. G.
Affiliations: University of California, Berkeley (US)
Corresponding author: Drubin, David G.
ORCID: Kaksonen, Marko
Toret, Christopher P.
Drubin, David G.
Venue: Cell
Publisher: Elsevier BV
Published: 2005-10
Volume: 123
Issue: 2
Pages: 305-320
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/j.cell.2005.09.024
OpenAlex: W2106252068
Cited by: 726
References: 0
Citations by year: 2026: 10 · 2025: 18 · 2024: 15 · 2023: 22 · 2022: 35 · 2021: 23 · 2020: 27 · 2019: 24 · 2018: 37 · 2017: 26 · 2016: 31 · 2015: 34 · 2014: 40 · 2013: 50 · 2012: 45
FWCI: 17.18
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 2882 d)
doi.org/10.15223/policy-017 (stm-asf)
doi.org/10.15223/policy-037 (stm-asf)
doi.org/10.15223/policy-012 (stm-asf)
doi.org/10.15223/policy-029 (stm-asf)
doi.org/10.15223/policy-004 (stm-asf)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Funders: Sigrid Juséliuksen Säätiö
National Institutes of Health (1s10rr019290-01)
National Institute of General Medical Sciences (R01GM042759, R01GM050399)
National Center for Research Resources (S10-RR019290)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords: clathrin-mediated endocytosis, Saccharomyces cerevisiae, deletion mutants, protein modules, membrane invagination, vesicle scission, protein dynamics
MeSH terms: Red Fluorescent Protein, Actins, Carrier Proteins, Cell Membrane, Clathrin, Cytoskeletal Proteins, Endocytosis, Fungal Proteins, Kinetics, Luminescent Proteins, Membrane Glycoproteins, Microfilament Proteins, Models, Biological, Saccharomyces cerevisiae, Biomarkers, Gene Deletion, Clathrin-Coated Vesicles, Saccharomyces cerevisiae Proteins, Green Fluorescent Proteins, Actin Capping Proteins

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A Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery

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