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Cited by 726Cellular transport and secretionFungal and yeast genetics researchMicrotubule and mitosis dynamics

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A Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery

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Kaksonen, Marko, Toret, Christopher P., Drubin, David G.·2005·Cell
DOI 10.1016/j.cell.2005.09.024
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Abstract

Endocytosis depends on an extensive network of interacting proteins that execute a series of distinct subprocesses. Previously, we used live-cell imaging of six budding-yeast proteins to define a pathway for association of receptors, adaptors, and actin during endocytic internalization. Here, we analyzed the effects of 61 deletion mutants on the dynamics of this pathway, revealing functions for 15 proteins, and we analyzed the dynamics of 8 of these proteins. Our studies provide evidence for four protein modules that cooperate to drive coat formation, membrane invagination, actin-meshwork assembly, and vesicle scission during clathrin/actin-mediated endocytosis. We found that clathrin facilitates the initiation of endocytic-site assembly but is not needed for membrane invagination or vesicle formation. Finally, we present evidence that the actin-meshwork assembly that drives membrane invagination is nucleated proximally to the plasma membrane, opposite to the orientation observed for previously studied actin-assembly-driven motility processes.

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Metadata

Authors
Kaksonen, M., Toret, C. P., & Drubin, D. G.
Affiliations
University of California, Berkeley (US)
Corresponding author
Drubin, David G.
ORCID
Venue
Cell
Publisher
Elsevier BV
Published
2005-10
Volume
123
Issue
2
Pages
305-320
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2005.09.024
OpenAlex
W2106252068
Cited by
726
References
0
Citations by year
2026: 10 · 2025: 18 · 2024: 15 · 2023: 22 · 2022: 35 · 2021: 23 · 2020: 27 · 2019: 24 · 2018: 37 · 2017: 26 · 2016: 31 · 2015: 34 · 2014: 40 · 2013: 50 · 2012: 45
FWCI
17.18
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Sigrid Juséliuksen Säätiö
  • National Institutes of Health (1s10rr019290-01)
  • National Institute of General Medical Sciences (R01GM042759, R01GM050399)
  • National Center for Research Resources (S10-RR019290)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
clathrin-mediated endocytosis, Saccharomyces cerevisiae, deletion mutants, protein modules, membrane invagination, vesicle scission, protein dynamics
MeSH terms
Red Fluorescent Protein, Actins, Carrier Proteins, Cell Membrane, Clathrin, Cytoskeletal Proteins, Endocytosis, Fungal Proteins, Kinetics, Luminescent Proteins, Membrane Glycoproteins, Microfilament Proteins, Models, Biological, Saccharomyces cerevisiae, Biomarkers, Gene Deletion, Clathrin-Coated Vesicles, Saccharomyces cerevisiae Proteins, Green Fluorescent Proteins, Actin Capping Proteins

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