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The Conserved Bardet-Biedl Syndrome Proteins Assemble a Coat that Traffics Membrane Proteins to Cilia

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Jin, Hua, White, Susan Roehl, Shida, Toshinobu, Schulz, Stefan, Aguiar, Mike, Gygi, Steven P., Bazan, J. Fernando, Nachury, Maxence V.·2010·Cell
DOI 10.1016/j.cell.2010.05.015
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Abstract

Summary The BBSome is a complex of Bardet-Biedl Syndrome (BBS) proteins that shares common structural elements with COPI, COPII and clathrin coats. Here we show that the BBSome constitutes a coat complex that sorts membrane proteins to primary cilia. Biochemically, the BBSome is the major effector of the Arf-like GTPase Arl6/ BBS3. In vivo, the BBSome and Arl6 localize to ciliary punctae and Arl6GTP is required to target the BBSome to cilia. Congruently, GTP-bound Arl6 and acidic phospholipids are sufficient to efficiently recruit the BBSome to chemically defined liposomes. Finally, ultrastructural analyses demonstrate that BBSome binding to liposomes produces distinct patches of polymerized coat. Since we establish that the ciliary targeting signal of somatostatin receptor 3 needs to be directly recognized by the BBSome to mediate targeting to cilia, we propose that trafficking to cilia entails the coupling of BBSome coat polymerization to the recognition of sorting signals.

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Research works printed but not linked: 50

  1. Berbari, 2008, Identification of ciliary localization sequences within the third intracellular loop of G protein-coupled receptors, Mol. Biol. Cell, 19, 1540, 10.1091/mbc.e07-09-0942 · DOI · Paper page
  2. Bielas, 2009, Mutations in INPP5E, encoding inositol polyphosphate-5-phosphatase E, link phosphatidyl inositol signaling to the ciliopathies, Nat. Genet., 41, 1032, 10.1038/ng.423 · DOI · Paper page
  3. Blacque, 2004, Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport, Genes Dev., 18, 1630, 10.1101/gad.1194004 · DOI · Paper page
  4. Bremser, 1999, Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors, Cell, 96, 495, 10.1016/S0092-8674(00)80654-6 · DOI · Paper page
  5. Chaudhuri, 2008, Evolution of the β-propeller fold, Proteins, 71, 795, 10.1002/prot.21764 · DOI
  6. Chiang, 2004, Comparative genomic analysis identifies an ADP-ribosylation factor-like gene as the cause of Bardet-Biedl syndrome (BBS3), Am. J. Hum. Genet., 75, 475, 10.1086/423903 · DOI · Paper page
  7. Christoforidis, 2000, Purification and identification of novel Rab effectors using affinity chromatography, Methods, 20, 403, 10.1006/meth.2000.0953 · DOI
  8. Fan, 2004, Mutations in a member of the Ras superfamily of small GTP-binding proteins causes Bardet-Biedl syndrome, Nat. Genet., 36, 989, 10.1038/ng1414 · DOI · Paper page
  9. Fliegauf, 2007, When cilia go bad: cilia defects and ciliopathies, Nat. Rev. Mol. Cell Biol., 8, 880, 10.1038/nrm2278 · DOI · Paper page
  10. Follit, 2010, The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence, J. Cell Biol., 188, 21, 10.1083/jcb.200910096 · DOI · Paper page
  11. Gillingham, 2007, The small G proteins of the Arf family and their regulators, Annu. Rev. Cell Dev. Biol., 23, 579, 10.1146/annurev.cellbio.23.090506.123209 · DOI · Paper page
  12. Haas, 2006, Optimization and use of peptide mass measurement accuracy in shotgun proteomics, Mol. Cell. Proteomics, 5, 1326, 10.1074/mcp.M500339-MCP200 · DOI · Paper page
  13. Jacoby, 2009, INPP5E mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in human and mouse, Nat. Genet., 41, 1027, 10.1038/ng.427 · DOI · Paper page
  14. Jínek, 2004, The superhelical TPR-repeat domain of O-linked GlcNAc transferase exhibits structural similarities to importin alpha, Nat. Struct. Mol. Biol., 11, 1001, 10.1038/nsmb833 · DOI · Paper page
  15. Kim, 2004, The Bardet-Biedl protein BBS4 targets cargo to the pericentriolar region and is required for microtubule anchoring and cell cycle progression, Nat. Genet., 36, 462, 10.1038/ng1352 · DOI · Paper page
  16. Kobayashi, 2009, Biochemical characterization of missense mutations in the Arf/Arl-family small GTPase Arl6 causing Bardet-Biedl syndrome, Biochem. Biophys. Res. Commun., 381, 439, 10.1016/j.bbrc.2009.02.087 · DOI
  17. Koenig, 1996, Synaptic vesicles have two distinct recycling pathways, J. Cell Biol., 135, 797, 10.1083/jcb.135.3.797 · DOI · Paper page
  18. Loktev, 2008, A BBSome subunit links ciliogenesis, microtubule stability, and acetylation, Dev. Cell, 15, 854, 10.1016/j.devcel.2008.11.001 · DOI · Paper page
  19. Mak, 2006, Polygenic control of Caenorhabditis elegans fat storage, Nat. Genet., 38, 363, 10.1038/ng1739 · DOI · Paper page
  20. Matsuoka, 1998, COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes, Cell, 93, 263, 10.1016/S0092-8674(00)81577-9 · DOI · Paper page
  21. Mazelova, 2009, Ciliary targeting motif VxPx directs assembly of a trafficking module through Arf4, EMBO J., 28, 183, 10.1038/emboj.2008.267 · DOI · Paper page
  22. McMahon, 2004, COP and clathrin-coated vesicle budding: different pathways, common approaches, Curr. Opin. Cell Biol., 16, 379, 10.1016/j.ceb.2004.06.009 · DOI · Paper page
  23. Milenkovic, 2009, Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium, J. Cell Biol., 187, 365, 10.1083/jcb.200907126 · DOI · Paper page
  24. Moritz, 2001, Mutant rab8 Impairs docking and fusion of rhodopsin-bearing post-Golgi membranes and causes cell death of transgenic Xenopus rods, Mol. Biol. Cell, 12, 2341, 10.1091/mbc.12.8.2341 · DOI · Paper page
  25. Nachury, 2010, Trafficking to the ciliary membrane: How to get across the periciliary diffusion barrier?, Annu. Rev. Cell Dev. Biol., 26, 10.1146/annurev.cellbio.042308.113337 · DOI · Paper page
  26. Ou, 2005, Functional coordination of intraflagellar transport motors, Nature, 436, 583, 10.1038/nature03818 · DOI · Paper page
  27. Rosenbaum, 2002, Intraflagellar transport, Nat. Rev. Mol. Cell Biol., 3, 813, 10.1038/nrm952 · DOI · Paper page
  28. Saffarian, 2009, Distinct dynamics of endocytic clathrin-coated pits and coated plaques, PLoS Biol., 7, e1000191, 10.1371/journal.pbio.1000191 · DOI · Paper page
  29. Santagata, 2001, G-protein signaling through tubby proteins, Science, 292, 2041, 10.1126/science.1061233 · DOI · Paper page
  30. Schermelleh, 2008, Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy, Science, 320, 1332, 10.1126/science.1156947 · DOI · Paper page
  31. Seo, 2009, Requirement of Bardet-Biedl syndrome proteins for leptin receptor signaling, Hum. Mol. Genet., 18, 1323, 10.1093/hmg/ddp031 · DOI · Paper page
  32. Seo, 2010, BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly, Proc. Natl. Acad. Sci. USA, 107, 1488, 10.1073/pnas.0910268107 · DOI · Paper page
  33. Spang, 1998, Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes, Proc. Natl. Acad. Sci. USA, 95, 11199, 10.1073/pnas.95.19.11199 · DOI · Paper page
  34. Stagg, 2007, Structural design of cage and coat scaffolds that direct membrane traffic, Curr. Opin. Struct. Biol., 17, 221, 10.1016/j.sbi.2007.03.010 · DOI
  35. Tam, 2000, Identification of an outer segment targeting signal in the COOH terminus of rhodopsin using transgenic Xenopus laevis, J. Cell Biol., 151, 1369, 10.1083/jcb.151.7.1369 · DOI · Paper page
  36. Wang, 2006, Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast, J. Cell Biol., 174, 973, 10.1083/jcb.200605106 · DOI
  37. Eswar, N., Webb, B., Marti-Renom, M.A., Madhusudhan, M.S., Eramian, D., Shen, M.Y., Pieper, U., and Sali, A. (2006). Comparative protein structure modeling using Modeler. Curr. Protoc. Bioinformatics Chapter 5 Unit 5.6. · DOI · Paper page
  38. Krissinel, E., and Henrick, K. (2004). Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions. Acta Crystallogr. D Biol. Crystallogr. 60, 2256–2268. · DOI · Paper page
  39. Lechtreck, K.F., Johnson, E.C., Sakai, T., Cochran, D., Ballif, B.A., Rush, J., Pazour, G.J., Ikebe, M., and Witman, G.B. (2009). The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J. Cell Biol. 187, 1117–1132. · DOI · Paper page
  40. Loktev, A.V., Zhang, Q., Beck, J.S., Searby, C.C., Scheetz, T.E., Bazan, J.F., Slusarski, D.C., Sheffield, V.C., Jackson, P.K., and Nachury, M.V. (2008). A BBSome subunit links ciliogenesis, microtubule stability, and acetylation. Dev. Cell 15, 854–865. · DOI · Paper page
  41. Malgaroli, A., and Tsien, R.W. (1992). Glutamate-induced long-term potentiation of the frequency of miniature synaptic currents in cultured hippocampal neurons. Nature 357, 134–139. · DOI · Paper page
  42. Mykytyn, K., Mullins, R.F., Andrews, M., Chiang, A.P., Swiderski, R.E., Yang, B., Braun, T., Casavant, T., Stone, E.M., and Sheffield, V.C. (2004). Bardet-Biedl syndrome type 4 (BBS4)-null mice implicate Bbs4 in flagella formation but not global cilia assembly. Proc. Natl. Acad. Sci. USA 101, 8664–8669. · DOI · Paper page
  43. Mykytyn, K., Nishimura, D.Y., Searby, C.C., Shastri, M., Yen, H.-J., Beck, J.S., Braun, T., Streb, L.M., Cornier, A.S., Cox, G.F., et al. (2002). Identification of the gene (BBS1) most commonly involved in Bardet-Biedl syndrome, a complex human obesity syndrome. Nat. Genet. 31, 435–438. · DOI · Paper page
  44. Nishimura, D.Y., Fath, M., Mullins, R.F., Searby, C., Andrews, M., Davis, R., Andorf, J.L., Mykytyn, K., Swiderski, R.E., Yang, B., et al. (2004). Bbs2-null mice have neurosensory deficits, a defect in social dominance, and retinopathy associated with mislocalization of rhodopsin. Proc. Natl. Acad. Sci. USA 101, 16588–16593. · DOI · Paper page
  45. Ou, G., Koga, M., Blacque, O.E., Murayama, T., Ohshima, Y., Schafer, J.C., Li, C., Yoder, B.K., Leroux, M.R., and Scholey, J.M. (2007). Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components into distinct modules based on transport and phenotypic profiles. Mol. Biol. Cell 18, 1554–1569. · DOI
  46. Pei, J., Kim, B.H., and Grishin, N.V. (2008). PROMALS3D: a tool for multiple protein sequence and structure alignments. Nucleic Acids Res. 36, 2295–2300. · DOI · Paper page
  47. Shimoni, Y., and Schekman, R. (2002). Vesicle budding from endoplasmic reticulum. Methods Enzymol. 351, 258–278. · DOI
  48. Söding, J. (2005). Protein homology detection by HMM-HMM comparison. Bioinformatics 21, 951–960. · DOI · Paper page
  49. Ventura, A., Meissner, A., Dillon, C.P., McManus, M., Sharp, P.A., Van Parijs, L., Jaenisch, R., and Jacks, T. (2004). Cre-lox-regulated conditional RNA interference from transgenes. Proc. Natl. Acad. Sci. USA 101, 10380–10385. · DOI · Paper page
  50. Zhang, Y. (2007). Template-based modeling and free modeling by I-TASSER in CASP7. Proteins 69 (Suppl 8), 108–117. · DOI · Paper page

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Metadata

Authors
Jin, H., White, S. R., Shida, T., Schulz, S., Aguiar, M., Gygi, S. P., Bazan, J. F., & Nachury, M. V.
Venue
Cell
Publisher
Elsevier BV
Published
2010-06
Volume
141
Issue
7
Pages
1208-1219
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2010.05.015
OpenAlex
W2161570516
Cited by
605
References
6
FWCI
29.17
Type
journal article
Language
en
Open access
bronze
Licence
Funders
  • March of Dimes Foundation (5-FY09-112)
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM089933, BR-5014, F32GM089218)
  • American Heart Association (AHA-0930365N)

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CoChat. (2026). Citation check: The Conserved Bardet-Biedl Syndrome Proteins Assemble a Coat that Traffics Membrane Proteins to Cilia. https://app.cochat.ai/papers/10.1016/j.cell.2010.05.015
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Jin, H., White, S. R., Shida, T., Schulz, S., Aguiar, M., Gygi, S. P., Bazan, J. F., & Nachury, M. V. (2010). The Conserved Bardet-Biedl Syndrome Proteins Assemble a Coat that Traffics Membrane Proteins to Cilia. Cell. https://doi.org/10.1016/j.cell.2010.05.015

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