Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 364

The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.

Identification of Ciliary Localization Sequences within the Third Intracellular Loop of G Protein-coupled Receptors

Open access
Berbari, Nicolas F., Johnson, Andrew D., Lewis, Jacqueline S., Askwith, Candice C., Mykytyn, Kirk·2008·Molecular Biology of the Cell
DOI 10.1091/mbc.e07-09-0942
Open this paper in CoChat — free account

Abstract

Primary cilia are sensory organelles present on most mammalian cells. The functions of cilia are defined by the signaling proteins localized to the ciliary membrane. Certain G protein–coupled receptors (GPCRs), including somatostatin receptor 3 (Sstr3) and serotonin receptor 6 (Htr6), localize to cilia. As Sstr3 and Htr6 are the only somatostatin and serotonin receptor subtypes that localize to cilia, we hypothesized they contain ciliary localization sequences. To test this hypothesis we expressed chimeric receptors containing fragments of Sstr3 and Htr6 in the nonciliary receptors Sstr5 and Htr7, respectively, in ciliated cells. We found the third intracellular loop of Sstr3 or Htr6 is sufficient for ciliary localization. Comparison of these loops revealed a loose consensus sequence. To determine whether this consensus sequence predicts ciliary localization of other GPCRs, we compared it with the third intracellular loop of all human GPCRs. We identified the consensus sequence in melanin-concentrating hormone receptor 1 (Mchr1) and confirmed Mchr1 localizes to primary cilia in vitro and in vivo. Thus, we have identified a putative GPCR ciliary localization sequence and used this sequence to identify a novel ciliary GPCR. As Mchr1 mediates feeding behavior and metabolism, our results implicate ciliary signaling in the regulation of body weight.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1091/mbc.e07-09-0942

Cites

7 of 7 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 7 references

Research works printed but not linked: 44

  1. Badano J. L., 2006, Annu. Rev. Genomics Hum. Genet, 7, 125, 10.1146/annurev.genom.7.080505.115610 · DOI · Paper page
  2. Bisgrove B. W., 2006, Development, 133, 4131, 10.1242/dev.02595 · DOI · Paper page
  3. Bishop G. A., 2007, J. Comp. Neurol, 505, 562, 10.1002/cne.21510 · DOI · Paper page
  4. Bloodgood R. A., 2000, Cell Biol. Int, 24, 857, 10.1006/cbir.2000.0598 · DOI
  5. Borowsky B., 2002, Nat. Med, 8, 825, 10.1038/nm741 · DOI · Paper page
  6. Chen Y., 2002, Endocrinology, 143, 2469, 10.1210/endo.143.7.8903 · doi:10.1210/endo.143.7.8903 · Paper page
  7. Davenport J. R., 2005, Am. J. Physiol. Renal. Physiol, 289, F1159, 10.1152/ajprenal.00118.2005 · DOI · Paper page
  8. Deretic D., 1998, Proc. Natl. Acad. Sci. USA, 95, 10620, 10.1073/pnas.95.18.10620 · DOI
  9. Dong C., 2007, Biochim. Biophys. Acta, 1768, 853, 10.1016/j.bbamem.2006.09.008 · DOI · Paper page
  10. Dwyer N. D., 2001, Neuron, 31, 277, 10.1016/S0896-6273(01)00361-0 · DOI · Paper page
  11. Egan C. T., 1998, J. Pharmacol. Exp. Ther, 286, 85, 10.1016/S0022-3565(24)37560-3 · DOI
  12. Gelber E. I., 1999, J. Neurochem, 72, 2206, 10.1046/j.1471-4159.1999.0722206.x · DOI
  13. Geng L., 2006, J. Cell Sci, 119, 1383, 10.1242/jcs.02818 · DOI · Paper page
  14. Gonnet G. H., 1992, Science, 256, 1443, 10.1126/science.1604319 · DOI · Paper page
  15. Hamon M., 1999, Neuropsychopharmacology, 21, 68S
  16. Handel M., 1999, Neuroscience, 89, 909, 10.1016/S0306-4522(98)00354-6 · DOI · Paper page
  17. Hermosilla R., 2001, Mol. Pharmacol, 60, 1031, 10.1124/mol.60.5.1031 · DOI
  18. Hildebrandt F., 2005, Nat. Rev. Genet, 6, 928, 10.1038/nrg1727 · DOI · Paper page
  19. Hipkin R. W., 2000, J. Biol. Chem, 275, 5591, 10.1074/jbc.275.8.5591 · doi:10.1074/jbc.275.8.5591
  20. Horn F., 2003, Nucleic Acids Res, 31, 294, 10.1093/nar/gkg103 · DOI · Paper page
  21. Horton R. M., 1990, Biotechniques, 8, 528
  22. 10.1091/mbc.e05-10-0935 · DOI
  23. Jenkins P. M., 2006, Curr. Biol, 16, 1211, 10.1016/j.cub.2006.04.034 · DOI · Paper page
  24. Kjelsberg M. A., 1992, J. Biol. Chem, 267, 1430, 10.1016/S0021-9258(18)45962-5 · DOI · Paper page
  25. Kohen R., 2001, Brain Res. Mol. Brain Res, 90, 110, 10.1016/S0169-328X(01)00090-0 · DOI
  26. Krogh A., 2001, J. Mol. Biol, 305, 567, 10.1006/jmbi.2000.4315 · DOI · Paper page
  27. Ludwig D. S., 2001, J. Clin. Invest, 107, 379, 10.1172/JCI10660 · DOI · Paper page
  28. Marshall W. F., 2006, Curr. Biol, 16, R604, 10.1016/j.cub.2006.07.012 · DOI · Paper page
  29. Marszalek J. R., 2000, Cell, 102, 175, 10.1016/S0092-8674(00)00023-4 · DOI · Paper page
  30. McEwen D. P., 2007, Proc. Natl. Acad. Sci. USA, 104, 15917, 10.1073/pnas.0704140104 · DOI · Paper page
  31. Oksenberg D., 1995, J. Neurochem, 64, 1440, 10.1046/j.1471-4159.1995.64041440.x · DOI
  32. Pan J., 2005, Lab. Invest, 85, 452, 10.1038/labinvest.3700253 · DOI · Paper page
  33. Patel Y. C., 1999, Front. Neuroendocrinol, 20, 157, 10.1006/frne.1999.0183 · DOI · Paper page
  34. Pazour G. J., 2003, Curr. Opin. Cell Biol, 15, 105, 10.1016/S0955-0674(02)00012-1 · DOI · Paper page
  35. Pissios P., 2006, Endocr. Rev, 27, 606, 10.1210/er.2006-0021 · DOI · Paper page
  36. Praetorius H. A., 2001, J. Membr. Biol, 184, 71, 10.1007/s00232-001-0075-4 · DOI · Paper page
  37. Qin H., 2005, Curr. Biol, 15, 1695, 10.1016/j.cub.2005.08.047 · DOI · Paper page
  38. Qu D., 1996, Nature, 380, 243, 10.1038/380243a0 · DOI · Paper page
  39. Ren Q., 1993, J. Biol. Chem, 268, 16483, 10.1016/S0021-9258(19)85445-5 · DOI · Paper page
  40. Rosenbaum J. L., 2002, Nat. Rev. Mol. Cell Biol, 3, 813, 10.1038/nrm952 · DOI · Paper page
  41. Schulz S., 2000, J. Physiol. Paris, 94, 259, 10.1016/S0928-4257(00)00212-6 · DOI
  42. Shimada M., 1998, Nature, 396, 670, 10.1038/25341 · DOI · Paper page
  43. Singla V., 2006, Science, 313, 629, 10.1126/science.1124534 · DOI · Paper page
  44. Takeda S., 2002, FEBS Lett, 520, 97, 10.1016/S0014-5793(02)02775-8 · DOI · Paper page

Cited by

See all 364

Metadata

Authors
Berbari, N. F., Johnson, A. D., Lewis, J. S., Askwith, C. C., & Mykytyn, K.
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2008-04
Volume
19
Issue
4
Pages
1540-1547
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e07-09-0942
OpenAlex
W2139886150
Cited by
364
References
7
FWCI
8.26
Type
journal article
Language
en
Open access
green

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Identification of Ciliary Localization Sequences within the Third Intracellular Loop of G Protein-coupled Receptors. https://app.cochat.ai/papers/10.1091/mbc.e07-09-0942
The paper
Berbari, N. F., Johnson, A. D., Lewis, J. S., Askwith, C. C., & Mykytyn, K. (2008). Identification of Ciliary Localization Sequences within the Third Intracellular Loop of G Protein-coupled Receptors. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e07-09-0942

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.