The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Induced Ectopic Kinetochore Assembly Bypasses the Requirement for CENP-A Nucleosomes
Open accessAbstract
Summary Accurate chromosome segregation requires assembly of the multi-protein kinetochore complex at centromeres. Although prior work identified the centromeric histone H3-variant CENP-A as the important upstream factor necessary for centromere specification, in human cells CENP-A is not sufficient for kinetochore assembly. Here, we demonstrate that two constitutive DNA-binding kinetochore components, CENP-C and CENP-T, function to direct kinetochore formation. Replacing the DNA-binding regions of CENP-C and CENP-T with alternate chromosome-targeting domains recruits these proteins to ectopic loci, resulting in CENP-A-independent kinetochore assembly. These ectopic kinetochore-like foci are functional based on the stoichiometric assembly of multiple kinetochore components including the microtubule-binding KMN network, the presence of microtubule attachments, the microtubule-sensitive recruitment of the spindle checkpoint protein Mad2, and the segregation behavior of foci-containing chromosomes. We additionally find that CENP-T phosphorylation regulates the mitotic assembly of both endogenous and ectopic kinetochores. Thus, CENP-C and CENP-T form a critical regulated platform for vertebrate kinetochore assembly.
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Self-citationThe human CENP-A centromeric nucleosome-associated complex
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From Silencing to Gene Expression
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Self-citationA Molecular View of Kinetochore Assembly and Function
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HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore
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Self-citationDrosophila CENH3 Is Sufficient for Centromere Formation
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Metadata
- Authors
- Gascoigne, K. E., Takeuchi, K., Suzuki, A., Hori, T., Fukagawa, T., & Cheeseman, I. M.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2011-04
- Volume
- 145
- Issue
- 3
- Pages
- 410-422
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2076095878
- Cited by
- 332
- References
- 37
- FWCI
- 14.30
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Gascoigne, K. E., Takeuchi, K., Suzuki, A., Hori, T., Fukagawa, T., & Cheeseman, I. M. (2011). Induced Ectopic Kinetochore Assembly Bypasses the Requirement for CENP-A Nucleosomes. Cell. https://doi.org/10.1016/j.cell.2011.03.031
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