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Cited by 612Cancer-related molecular mechanisms researchGenomics and Chromatin DynamicsEpigenetics and DNA Methylation

ncRNA- and Pc2 methylation-dependent gene relocation between nuclear structures mediates gene activation programs

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Yang, Liuqing, Lin, Chunru, Liu, Wen, Zhang, Jie, Ohgi, Kenneth A., Grinstein, Jonathan D., Dorrestein, Pieter C., Rosenfeld, Michael G.·2011·Cell
DOI 10.1016/j.cell.2011.08.054PMC PMC3297197
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Abstract

Although eukaryotic nuclei contain distinct architectural structures associated with noncoding RNAs (ncRNAs), their potential relationship to regulated transcriptional programs remains poorly understood. Here, we report that methylation/demethylation of Polycomb 2 protein (Pc2) controls relocation of growth-control genes between Polycomb bodies (PcGs) and interchromatin granules (ICGs) in response to growth signals. This movement is the consequence of binding of methylated and unmethylated Pc2 to the ncRNAs TUG1 and MALAT1/NEAT2, located in PcGs and ICGs, respectively. These ncRNAs mediate assembly of multiple corepressors/coactivators and can serve to switch mark recognition by "readers" of the histone code. Additionally, binding of NEAT2 to unmethylated Pc2 promotes E2F1 SUMOylation, leading to activation of the growth-control gene program. These observations delineate a molecular pathway linking the actions of subnuclear structure-specific ncRNAs and nonhistone protein methylation to relocation of transcription units in the three-dimensional space of the nucleus, thus achieving coordinated gene expression programs.

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  • ncRNAs, due to their conspicuous amount and proportion in the transcriptome, play a central role in epigenetics with an incidence in all functional levels.

    “Overall, ncRNAs, due to their conspicuous amount and proportion in the transcriptome, play a central role in epigenetics with an incidence in all functional levels (Figure 2).”

    From Revisiting Epigenetics Fundamentals and Its Biomedical Implications (2024) · cites this paper as [81]

    The Introduction (page unavailable) describes long ncRNAs as a large class of transcriptional units, and the Results and Discussion (pages unavailable) show that specific ncRNAs participate in chromatin regulation and gene-expression programs. The paper does not establish their proportion of the transcriptome or show that they act at all functional levels of epigenetics; its findings concern specific ncRNAs and mechanisms. (LLM, conf=0.85)

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Sorted by citations

Authors
Yang, L., Lin, C., Liu, W., Zhang, J., Ohgi, K. A., Grinstein, J. D., Dorrestein, P. C., & Rosenfeld, M. G.
Affiliations
  • Howard Hughes Medical Institute (US)
  • University of California San Diego (US)
Corresponding author
Rosenfeld, Michael G.
ORCID
Venue
Cell
Publisher
Elsevier BV
Published
2011-11
Volume
147
Issue
4
Pages
773-788
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2011.08.054
PMC
PMC3297197
OpenAlex
W2169416332
Cited by
612
References
7
Citations by year
2026: 7 · 2025: 13 · 2024: 16 · 2023: 22 · 2022: 25 · 2021: 35 · 2020: 46 · 2019: 50 · 2018: 44 · 2017: 56 · 2016: 59 · 2015: 67 · 2014: 73 · 2013: 56 · 2012: 40
FWCI
18.61
Type
journal article
Language
en
Update notices
erratum (2013-10-10)
Open access
Licence
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • U.S. Department of Defense
  • Howard Hughes Medical Institute
  • Prostate Cancer Foundation
  • Susan G. Komen
  • National Heart, Lung, and Blood Institute (R01HL065445)
  • National Cancer Institute (R01CA097134, CA97134)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK39949, R01DK039949, DK018477, P01DK074868, R37DK039949, DK74868, R01DK018477)
  • National Institute of Neurological Disorders and Stroke (R01NS034934, NS 34934)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
non-coding RNA, DNA methylation, interchromatin granules, nuclear organization, TUG1, MALAT1, gene activation, histone code, transcriptional regulation
MeSH terms
Amino Acid Sequence, Cell Line, Cell Nucleus, Chromatin, Gene Expression Regulation, HeLa Cells, Humans, Ligases, Methylation, Methyltransferases, Molecular Sequence Data, Repressor Proteins, Transcription, Genetic, RNA, Untranslated, Ubiquitin-Protein Ligases, E2F1 Transcription Factor, Ubiquitination, Sumoylation, RNA, Long Noncoding, Polycomb-Group Proteins

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