Vesicle-Mediated Steroid Hormone Secretion in Drosophila melanogaster
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Steroid hormones are a large family of cholesterol derivatives regulating development and physiology in both the animal and plant kingdoms, but little is known concerning mechanisms of their secretion from steroidogenic tissues. Here, we present evidence that in Drosophila, endocrine release of the steroid hormone ecdysone is mediated through a regulated vesicular trafficking mechanism. Inhibition of calcium signaling in the steroidogenic prothoracic gland results in the accumulation of unreleased ecdysone, and the knockdown of calcium-mediated vesicle exocytosis components in the gland caused developmental defects due to deficiency of ecdysone. Accumulation of synaptotagmin-labeled vesicles in the gland is observed when calcium signaling is disrupted, and these vesicles contain an ABC transporter that functions as an ecdysone pump to fill vesicles. We propose that trafficking of steroid hormones out of endocrine cells is not always through a simple diffusion mechanism as presently thought, but instead can involve a regulated vesicle-mediated release process.
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In Drosophila, ecdysone is released out of cells via an ABC protein that functions as an ecdysone transporter.
“In Drosophila, ecdysone is released out of cells via ABC protein that functions as an ecdysone transporter [66, 67].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [66]
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- Authors
- Yamanaka, N., Marqués, G., & O’Connor, M. B.
- Affiliations
- University of California, Riverside (US)
- University of Minnesota (US)
- Corresponding author
- Yamanaka, Naoki
- O’Connor, Michael B.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2015-11
- Volume
- 163
- Issue
- 4
- Pages
- 907-919
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC4636736
- OpenAlex
- W2112092845
- Cited by
- 147
- References
- 66
- Citations by year
- 2026: 7 · 2025: 19 · 2024: 14 · 2023: 13 · 2022: 14 · 2021: 22 · 2020: 15 · 2019: 7 · 2018: 15 · 2017: 13 · 2016: 8
- FWCI
- 6.39
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM093301)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (K99/R00 HD073239, R00HD073239, K99HD073239)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- steroid secretion, ecdysone, prothoracic gland, vesicle exocytosis, calcium signaling, regulated secretion, synaptotagmin, ABC transporters, vesicle trafficking, Drosophila melanogaster
- MeSH terms
- Adenosine Triphosphate, Animals, Diffusion, Drosophila melanogaster, Ecdysone, Endocrine Glands, Exocytosis, Larva, ATP-Binding Cassette Transporters, Secretory Vesicles, Drosophila Proteins, Synaptotagmins, Inositol 1,4,5-Trisphosphate Receptors, Gene Knockdown Techniques
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