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Cited by 365

The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.

Sugar Synthesis from CO2 in Escherichia coli

Open access
Antonovsky, Niv, Gleizer, Shmuel, Noor, Elad, Zohar, Yehudit, Herz, Elad, Barenholz, Uri, Zelcbuch, Lior, Amram, Shira, Wides, Aryeh, Tepper, Naama, Davidi, Dan, Bar-On, Yinon, Bareia, Tasneem, Wernick, David G., Shani, Ido, Malitsky, Sergey, Jona, Ghil, Bar-Even, Arren, Milo, Ron·2016·Cell
DOI 10.1016/j.cell.2016.05.064
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Abstract

Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and laboratory evolution has led to the biosynthesis of sugars and other major biomass constituents by a fully functional Calvin-Benson-Bassham (CBB) cycle in E. coli. In the evolved bacteria, carbon fixation is performed via a non-native CBB cycle, while reducing power and energy are obtained by oxidizing a supplied organic compound (e.g., pyruvate). Genome sequencing reveals that mutations in flux branchpoints, connecting the non-native CBB cycle to biosynthetic pathways, are essential for this phenotype. The successful evolution of a non-native carbon fixation pathway, though not yet resulting in net carbon gain, strikingly demonstrates the capacity for rapid trophic-mode evolution of metabolism applicable to biotechnology. PAPERCLIP.

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Metadata

Authors
Antonovsky, N., Gleizer, S., Noor, E., Zohar, Y., Herz, E., Barenholz, U., Zelcbuch, L., Amram, S., Wides, A., Tepper, N., Davidi, D., Bar-On, Y., Bareia, T., Wernick, D. G., Shani, I., Malitsky, S., Jona, G., Bar-Even, A., & Milo, R.
Venue
Cell
Publisher
Elsevier BV
Published
2016-06
Volume
166
Issue
1
Pages
115-125
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2016.05.064
OpenAlex
W2468352349
Cited by
365
References
39
FWCI
17.15
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Sugar Synthesis from CO2 in Escherichia coli. https://app.cochat.ai/papers/10.1016/j.cell.2016.05.064
The paper
Antonovsky, N., Gleizer, S., Noor, E., Zohar, Y., Herz, E., Barenholz, U., Zelcbuch, L., Amram, S., Wides, A., Tepper, N., Davidi, D., Bar-On, Y., Bareia, T., Wernick, D. G., Shani, I., Malitsky, S., Jona, G., Bar-Even, A., & Milo, R. (2016). Sugar Synthesis from CO2 in Escherichia coli. Cell. https://doi.org/10.1016/j.cell.2016.05.064

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.