The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Replication-Transcription Conflicts Generate R-Loops that Orchestrate Bacterial Stress Survival and Pathogenesis
Open accessAbstract
SUMMARY Replication-transcription collisions shape genomes, influence evolution, and promote genetic diseases. Although unclear why, head-on transcription (lagging strand genes) is especially disruptive to replication and promotes genomic instability. Here, we find that head-on collisions promote R-loop formation in Bacillus subtilis. We show that pervasive R-loop formation at head-on collision regions completely blocks replication, elevates mutagenesis, and inhibits gene expression. Accordingly, the activity of the R-loop processing enzyme RNase HIII at collision regions is crucial for stress survival in B. subtilis, as many stress response genes are head-on to replication. Remarkably, without RNase HIII, the ability of the intracellular pathogen Listeria monocytogenes to infect and replicate in hosts is weakened significantly, most likely because many virulence genes are head-on to replication. We conclude that the detrimental effects of head-on collisions stem primarily from excessive R-loop formation and that the resolution of these structures is critical for bacterial stress survival and pathogenesis.
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Condition-Dependent Transcriptome Reveals High-Level Regulatory Architecture in Bacillus subtilis
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Gene essentiality determines chromosome organisation in bacteria
Rocha, E. P. C. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg859 · Cited by 166
Molecular diversities of RNases H
Ohtani, N., Haruki, M., Morikawa, M., & Kanaya, S. (1999) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(99)80168-6 · Cited by 114
Cited by
R-Loops as Cellular Regulators and Genomic Threats
Crossley, M. P., Bocek, M., & Cimprich, K. A. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.01.024 · Cited by 746
R Loops: From Physiological to Pathological Roles
García-Muse, T., & Aguilera, A. (2019) · Cell · doi:10.1016/j.cell.2019.08.055 · Cited by 697
Mechanisms of Oncogene-Induced Replication Stress: Jigsaw Falling into Place
Kotsantis, P., Petermann, E., & Boulton, S. J. (2018) · Cancer Discovery · doi:10.1158/2159-8290.cd-17-1461 · Cited by 416
The DNA damage response to transcription stress
Lans, H., Hoeijmakers, J. H. J., Vermeulen, W., & Marteijn, J. A. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0169-4 · Cited by 321
Zatreanu, D., Han, Z., Mitter, R., Tumini, E., Williams, H., Gregersen, L., Dirac-Svejstrup, A. B., Roma, S., Stewart, A., Aguilera, A., et al. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.07.037 · Cited by 124
Metadata
- Authors
- Lang, K. S., Hall, A. N., Merrikh, C. N., Ragheb, M., Tabakh, H., Pollock, A. J., Woodward, J. J., Dreifus, J. E., & Merrikh, H.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2017-08
- Volume
- 170
- Issue
- 4
- Pages
- 787-799.e18
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2742880086
- Cited by
- 250
- References
- 4
- FWCI
- 16.53
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Lang, K. S., Hall, A. N., Merrikh, C. N., Ragheb, M., Tabakh, H., Pollock, A. J., Woodward, J. J., Dreifus, J. E., & Merrikh, H. (2017). Replication-Transcription Conflicts Generate R-Loops that Orchestrate Bacterial Stress Survival and Pathogenesis. Cell. https://doi.org/10.1016/j.cell.2017.07.044
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