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Quantifying Absolute Protein Synthesis Rates Reveals Principles Underlying Allocation of Cellular Resources

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Li, Gene-Wei, Burkhardt, David, Gross, Carol, Weissman, Jonathan S.·2014·Cell
DOI 10.1016/j.cell.2014.02.033
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Abstract

Quantitative views of cellular functions requires precise measures of rates of biomolecule production, especially proteins—the direct effectors of biological processes. Here we present a genome-wide approach, based on ribosome profiling, for measuring absolute protein synthesis rates. The resultant E. coli dataset transforms our understanding of the extent to which protein synthesis is precisely controlled to optimize function and efficiency. Members of multi-protein complexes are made in precise proportion to their stoichiometry, whereas components of functional modules are produced differentially according to their hierarchical role. Estimates of absolute protein abundance also reveal principles used to optimize design. These include how the level of different types of transcription factors is optimized for rapid response, and how a metabolic pathway (methionine biosynthesis) balances production cost with activity requirements. Our studies reveal how general principles, important both for understanding natural systems and for synthesizing new ones, emerge from quantitative analyses of protein synthesis.

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Metadata

Authors
Li, G.-W., Burkhardt, D., Gross, C., & Weissman, J. S.
Venue
Cell
Publisher
Elsevier BV
Published
2014-04
Volume
157
Issue
3
Pages
624-635
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2014.02.033
OpenAlex
W2061659241
Cited by
1,251
References
15
FWCI
38.57
Type
journal article
Language
en
Open access
bronze
Licence

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Li, G.-W., Burkhardt, D., Gross, C., & Weissman, J. S. (2014). Quantifying Absolute Protein Synthesis Rates Reveals Principles Underlying Allocation of Cellular Resources. Cell. https://doi.org/10.1016/j.cell.2014.02.033

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